Biologia plantarum 2010, 54:671-676 | DOI: 10.1007/s10535-010-0119-7

Alteration in growth and thylakoid membrane lipid composition of Azolla caroliniana under phosphate deficiency

G. S. M. Ismail1,*, H. E. Mohamed1
1 Botany Department, Faculty of Science, Alexandria University, Alexandria, Egypt

The changes in the fresh biomass accumulation, photosynthetic and anthocyanin pigments, photosystem 2 (PS 2) activity, ultrastructure of chloroplast, total lipids and fatty acid composition of thylakoid membrane were followed in the aquatic fern Azolla caroliniana grown on medium either deficient or supplied with various phosphorus concentrations. The content of photosynthetic pigments and the anthocyanin/chlorophyll ratio increased significantly with increasing PO4 3- concentration. Phosphate deficiency inhibited growth and PS 2 activity and decreased content of total lipids and phospholipids in isolated thylakoids. This was accompanied with a significant increase in the percentage of galalactolipids.

Keywords: anthocyanin; aquatic fern; chlorophyll; chloroplast ultrastructure; fatty acids; galactolipids; phospholipids
Subjects: aldose reductase; anthocyanins; aquatic fern; Azolla caroliniana; carotenoids; chlorophyll a,b; chloroplast; fatty acids; galactolipids; lipids; phospholipids; phosphorus; photosystems (PS 1, PS 2); proteins; salicylic acid; salinity; sorbitol; tomato

Received: May 1, 2009; Accepted: December 15, 2009; Published: December 1, 2010Show citation

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Ismail, G.S.M., & Mohamed, H.E. (2010). Alteration in growth and thylakoid membrane lipid composition of Azolla caroliniana under phosphate deficiency. Biologia plantarum, 54(4), 671-676. doi: 10.1007/s10535-010-0119-7.
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    References

    1. Adalberto, P.R., Massabni, A.C., Goulart, A.J., Monti, R., Lacava, P.M.: [Effect of phosphorus on the mineral uptake and pigmentation of Azolla caroliniana Willd. (Azollaceae).] - Rev. bras. bot. 27: 581-585, 2004. [In Port.] Go to original source...
    2. Ajay-Jain, A., Cao, A., Karthikeyan, A.S., Baldwin, J.C., Raghothama, K.G.: Phosphate deficiency suppresses expression of light-regulated psbO and psbP genes encoding extrinsic proteins of oxygen-evolving complex of PSII. - Curr. Sci. 89: 1592-1596, 2005.
    3. Akhtar, M.S., Oki, Y., Adachi, T.: Genetic diversity in Brassica cultivars under deficiently buffered P-stress environment: I. Biomass accumulation, P-concentration, P-uptake, and related growth parameters. - J. amer. Sci. 3: 55-63, 2007.
    4. Andersson, M.X., Larsson, K.E., Tjellstrom, H., Liljenberg, C., Sandelius, A.S.: Phosphate-limited oat. - J. biol. Chem. 280: 27578-27586, 2005. Go to original source...
    5. Andersson, M.X., Stridh, M.H., Larsson, K. E., Liljenberg, C., Sandelius, A.S.: Phosphate-deficient oat replaces a major portion of the plasma membrane phospholipids with the galactolipid digalactosyldiacylglycerol. - FEBS Lett. 537: 128-132, 2003. Go to original source...
    6. Augustin, O.P.H.: Differentiation between yeast species, and strains within a species by cellular fatty acid analysis. 2 Saccharomycetes cerevisiae. - South Afr. J. Enol. Viticult. 10: 8-15, 1989.
    7. Biswall, U.C., Mohanty, P.: Aging induced changes in photosynthetic electron transport of detached barley leaves. - Plant Cell Physiol. 17: 323-332, 1976.
    8. De Santis, A., Landi, P., Genchi, G.: Changes in mitochondrial properties in maize seedlings associated with selection for germination at low temperature. Fatty acid composition, cytochrome oxidase and nucleotide translocase activities. - Plant Physiol. 119: 743-754, 1999. Go to original source...
    9. Doncheva, S., Vassileva, V., Ignatov, G., Pandev, S.: Influence of nitrogen deficiency on photosynthesis and chloroplast ultrastructure of pepper plants. - Agr. Food Sci. Finland 10: 59-64, 2001.
    10. Dörmann, P., Benning, C.: Galactolipids rule in seed plants. - Trends Plant Sci. 7: 112-118, 2002. Go to original source...
    11. El-Sheek, M.M., Rady, A.A.: Effect of phosphorus starvation on growth, photosynthesis and some metabolic processes in the unicellular alga Chlorella kessleri. - Phyton 35: 139-151, 1995.
    12. Essigmann, B., Güler, S., Narang, R.A., Linke, D., Benning, C.: Phosphate availability affects the thylakoid lipid composition and the expression of SQD1, a gene required for sulfolipid biosynthesis in Arabidopsis thaliana. - Proc. nat. Acad. Sci. USA 95: 1950-1955, 1998.
    13. Feild, T.S., Lee, D.W., Holbrook, N.M.: Why leaves turn red in autumn. The role of anthocyanins in senescing leaves of red-osier dogwood. - Plant Physiol. 127: 566-574, 2001. Go to original source...
    14. Foyer, C., Spencer, C.: The relationship between phosphate status and photosynthesis in leaves: Effects on intracellular orthophosphate distribution, photosynthesis and assimilate partitioning. - Planta 167: 369-375, 1986. Go to original source...
    15. Fredeen, A. L., Rao, I.M., Terry, N.: Influence of phosphorus nutrition on growth and carbon partitioning in Glycine max. - Plant Physiol. 89: 225-230, 1989. Go to original source...
    16. Hagio, M., Gombos, Z., Varkonyi, Z., Masamoto, K., Sato, N., Tsuzuki, M., Wada, H.: Direct evidence for requirement of phosphatidylglycerol in photosystem II of photosynthesis. - Plant Physiol. 124: 795-804, 2000. Go to original source...
    17. Hall, J.D., Barr, R., Al-Abbas, A.H., Crane, F.L.: The ultrastructure of chloroplasts in mineral-deficient maize leaves. - Plant Physiol. 50: 404-409, 1972. Go to original source...
    18. Hartel, H., Dormann, P., Benning, C.: DGD1-independent biosynthesis of extraplastidic galactolipids after phosphate deprivation in Arabidopsis. - Proc. nat. Acad. Sci. USA 97: 10649-10654, 2000. Go to original source...
    19. Jouhet, J., Maréchal, E., Baldan, B., Bligny, R., Joyard, J., Block, M. A.: Phosphate deprivation induces transfer of DGDG galactolipid from chloroplast to mitochondria. - J. cell. Biol. 167: 863-874, 2004. Go to original source...
    20. Jouhet, J., Maréchal, E., Bligny, R., Joyard, J., Block, M.A.: Transient increase of phosphatidylcholine in plant cells in response to phosphate deprivation. - FEBS Lett. 544: 63-68, 2003. Go to original source...
    21. Khozin-Goldberg, K., Cohen, Z.: The effect of phosphate starvation on the lipid and fatty acid composition of the fresh water eustigmatophyte Monodus subterraneus. - Phytochemistry 67: 696-701, 2006. Go to original source...
    22. Kobayashi, K., Masuda, T., Takamiya, K., Ohta, H.: Membrane lipid alteration during phosphate starvation is regulated by phosphate signaling and auxin/cytokin cross-talk. - Plant J. 47: 238-248, 2006. Go to original source...
    23. Kock, J.L.F., Botes, P.J., Erasmus, S.C., Lategan, P.M.: A rapid method to differentiate between four species of Endomycetaceae. - J. gen. Microbiol. 131: 3393-3396, 1985. Go to original source...
    24. Li, M., Welti, R., Wang, X.: Quantitative profiling of Arabidopsis polar glycerolipids in response to phosphorus starvation. Roles of phospholipases Dz1 and Dz2 in phosphatidylcholine hydrolysis and digalactosyldiacylglycerol accumulation in phosphorus-starved plants. - Plant Physiol. 142: 750-761, 2006. Go to original source...
    25. Metzner, H., Rauh, H.J., Senger H.: The synchronization of some pigment-deficient mutants of Chlorella. - Planta 65: 186-190, 1965. Go to original source...
    26. Melton, S., Moyers, R., Play-Ford, C.: Lipid extracted from soy bean products by different procedures. - J. amer. oil chem. Soc. 56: 489-493, 1979. Go to original source...
    27. Osman, M.E.H., El-Shintinawy, F.: Photosynthetic electron transport under phosphorylating conditions of various salts. - J. exp. Bot. 39: 859-863, 1988. Go to original source...
    28. Plesnicar, M., Kastori, R., Petrovic, N., Pankovic, D.: Photosynthesis and chlorophyll fluorescence in sunflower (Helianthus annuus L.) leaves as affected by phosphorus nutrition. - J. exp. Bot. 45: 919-924, 1994. Go to original source...
    29. Raghothama, K.G.: Phosphate acquisition. - Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 665-693, 1999. Go to original source...
    30. Raghothama, K.G., Karthikeyan, A.S.: Phosphate acquisition. - Plant Soil 274: 37-49, 2005. Go to original source...
    31. Rao, I.M.: The role of phosphorus in photosynthesis. - In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 173-194. Marcel Dekker, New York 1997.
    32. Rao, I.M., Terry, N.: Leaf phosphate status, photosynthesis, and carbon partitioning in sugar beet: I. Changes in growth, gas exchange, and Calvin cycle enzymes. - Plant Physiol. 90: 814-819, 1989. Go to original source...
    33. Ripley, B.S., Redfern, S.P., Dames, J.: Quantification of the photosynthetic performance of phosphorus-deficient Sorghum by means of chlorophyll-a fluorescence kinetics. - South Afr. J. Sci. 100: 615-618, 2004.
    34. Siegenthaler, P.A.: Molecular organization of acyllipids in photosynthetic membranes of higher plants. - In: Siegenthaler, P.A., Murata, N. (ed.): Lipids in Photosynthesis. Pp. 119-144. Kluwer Academic Publishers, Dordrecht 1998.
    35. Siron, R., Giusti, G., Berland, B.: Changes in the fatty acid composition of Phaeodactylum tricornutum and Dunaliella tertiolecta during growth and under phosphorus deficiency. - Mar. Ecol. 55: 95-100, 1989. Go to original source...
    36. Stewart, E.A., Grimshaw, H.M.: Chemical analysis of ecological materials. - In: Stewart, E.A. (ed.). Phosphorus and Compounds. Pp. 206-209. Blackwell, Madison 1974.
    37. Thomson, W.W., Weier, T.E., Drever, H.: The fine structure of chloroplasts from mineral deficient leaves of Phaseolus vulgaris. - Amer. J. Bot. 49: 1047-1055, 1964.
    38. Ticconi, C.A., Delatorre, C.A., Abel, S.: Attenuation of phosphate starvation responses by phosphite in Arabidopsis. - Plant Physiol. 127: 963-972, 2001. Go to original source...
    39. Tsvetkova, G.E., Georgiev, G.I.: Effect of phosphorus nutrition on the nodulation, nitrogen fixation and nutrient-use efficiency of Bradyrhizobium japonicum-soybean (Glycine max L. Merr) symbiosis. - Bulg. J. Plant Physiol. 2003(Special issue): 331-335, 2003.
    40. Vance, C.P., Uhde-Stone, C., Allan, D.H.: Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. - New Phytol. 157: 423-47, 2003. Go to original source...
    41. Yan, F.R., Schaffer, S., Fortmeier, H., Schubert, S.: Adaptation of active proton pumping and plasmalemma ATP-ase activity of corn roots to low root medium pH. - Plant Physiol. 117: 311-319, 1998. Go to original source...
    42. Yang, W., Liu, S., Feng, F., Hou, H., Jiang, G., Xu, Y., Kuang, T.: Effects of phosphate deficiency on the lipid composition in cucumber thylakoid membranes and PSII particles. - Plant Sci. 166: 1575-1579, 2004. Go to original source...
    43. Zaheer, A., Gill, M.A., Qureshi, R.H.: Genotypic variations of phosphorus utilization efficiency of crops. - J. Plant Nutr. 24: 1149-1171, 2001.
    44. Zhou, Y.H., Wu, J.X., Zhu, L.J., Shi, K., Yu, J.Q.: Effects of phosphorus and chilling under low irradiance on photosynthesis and growth of tomato plants. - Biol. Plant. 53: 378-382, 2009. Go to original source...

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