Biologia plantarum 57:33-40, 2013 | DOI: 10.1007/s10535-012-0234-8

Photosynthetic parameters of Ulmus minor plantlets affected by irradiance during acclimatization

M. C. Dias1,*, G. Pinto1, C. M. Correia2, J. Moutinho-Pereira2, S. Silva1, C. Santos1
1 Department of Biology and Centre for Environmental and Marine Studies (CESAM), University of Aveiro, Aveiro, Portugal
2 Centre for Research and Technology in Agro-Environmental and Biological Sciences (CITAB) and Department of Biology and Environment, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal

In order to set up large-scale acclimatization protocols of micropropagated plants, an in-depth knowledge of their physiological responses during in vitro to ex vitro transfer is required. This work describes the photosynthetic performance of Ulmus minor micropropagated plants during acclimatization at high irradiance (HI; 200 ± 20 μmol m-2 s-1 or low irradiance (LI; 100 ± 20 μmol m-2 s-1). During this experiment, leaf pigment content, chlorophyll a fluorescence, gas exchange, stomata morphology, the activity of the Calvin cycle enzymes and saccharides were measured in persistent and new leaves. The results indicated that HI induces a higher photosynthetic performance compared to LI. Therefore, plants acclimatized under HI are likely to survive better after field transfer.

Keywords: Calvin cycle enzymes; chlorophyll; elm; fluorescence; micropropagation; net photosynthetic rate; stomata; transpiration rate
Subjects: irradiance; photosynthesis; ex vitro acclimatization; Calvin cycle enzymes; chlorophyll; elm; chlorophyll fluorescence; in vitro culture; net photosynthetic rate; stomatal conductance; transpiration rate
Species: Ulmus minor

Received: August 11, 2011; Accepted: February 8, 2012; Published: March 1, 2013Show citation

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Dias, M.C., Pinto, G., Correia, C.M., Moutinho-Pereira, J., Silva, S., & Santos, C. (2013). Photosynthetic parameters of Ulmus minor plantlets affected by irradiance during acclimatization. Biologia plantarum, 57(1), 33-40. doi: 10.1007/s10535-012-0234-8.
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