Biologia plantarum 57:179-183, 2013 | DOI: 10.1007/s10535-012-0257-1

Ectopic expression of the Osmyb4 rice gene enhances synthesis of hydroxycinnamic acid derivatives in tobacco and clary sage

T. Docimo1,*, M. Mattana1, R. Fasano2, R. Consonni3, N. de Tommasi2, I. Coraggio, A. Leone2
1 Istituto di Biologia e Biotecnologie Agrarie, Consiglio Nazionale delle Ricerche, Milano, Italia
2 Facoltà di Farmacia, Università di Salerno, Fisciano, Italia
3 Istituto per lo Studio delle Macromolecole, Consiglio Nazionale delle Ricerche, Milano, Italia

In this work, we report the ectopic expression of the Osmyb4 rice gene, encoding the Myb4 transcription factor, in Nicotiana tabacum and Salvia sclarea. Transcriptional analysis of T2 homozygous tobacco plants overexpressing Osmyb4 revealed that Myb4 activated the transcription of several genes of the phenylpropanoid pathway such as PAL, C4H, 4CL1, 4CL2 (encoding phenylalanine ammonia-lyase, cinnamic acid 4-hydroxylase, 4-coumarate: Co A ligase1, 4-coumarate: Co A ligase2). Moreover, the Myb4 increased expression of HQT encoding hydroxycinnamoyl-CoA: quinate transferase, which specifically triggers the accumulation of chlorogenic acid (CGA). In addition, increased acccumulation of rosmarinic acid (RA) was found in transgenic plants of both species. These results open the possibility of using the Osmyb4 gene to increase the production of specific bioactive hydroxycinnamates.

Keywords: chlorogenic acid; Myb transcription factor; Nicotiana tabacum; phenylpropanoid pathway; rosmarinic acid; Salvia sclarea
Subjects: gene expression; transgenic plants; Osmyb4 rice gene; hydroxycinnamic acid; chlorogenic acid; Myb transcription factor; phenylpropanoid pathway; rosmarinic acid; tobacco; clary sage
Species: Nicotiana tabacum; Oryza sativa; Salvia sclarea

Received: December 21, 2011; Accepted: April 19, 2012; Published: March 1, 2013Show citation

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Docimo, T., Mattana, M., Fasano, R., Consonni, R., de Tommasi, N., Coraggio, I., & Leone, A. (2013). Ectopic expression of the Osmyb4 rice gene enhances synthesis of hydroxycinnamic acid derivatives in tobacco and clary sage. Biologia plantarum, 57(1), 179-183. doi: 10.1007/s10535-012-0257-1.
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