Biologia plantarum 57:365-369, 2013 | DOI: 10.1007/s10535-012-0275-z

Calcium-mediated enhancement of copper tolerance in Elodea canadensis

H. L. Min1, S. J. Cai1, Z. Rui1, S. Sha1, K. B. Xie1, Q. S. Xu1,*
1 Institute of Plant Cell and Molecular Biology, College of Life Science, Nanjing Normal University, Nanjing, P.R.China

The alleviative effects of exogenous calcium on copper phytotoxicity were investigated in Elodea canadensis plants. There was a significant accumulation of Cu in the plants after their exposure to 0.01 mM Cu accompanied by many symptoms of toxicity. Increased uptake of Cu severely reduced content of photosynthetic pigments, soluble proteins, and free proline. The total antioxidant capacity (T-AOC), reduced glutathione (GSH), and non-protein thiol (NP-SH) were severely suppressed in Cu-stressed plants resulting in a rapid increase in content of superoxide anion (O2 .-), hydrogen peroxide, lipid peroxidation, and cell death. Simultaneous application of Ca markedly increased the content of photosynthetic pigments, soluble proteins, free proline, T-AOC, GSH, and NP-SH, and reduced oxidative damage as indicated by lowered content of MDA, O2 .-, and H2O2; and decreased cell death. Furthermore, application of Ca reduced Cu uptake and effectively reversed the Cu-induced nutrient imbalance.

Keywords: alleviation; antioxidants; carotenoids; chlorophyll; glutathione; H2O2; mineral nutrition; O2.-; proline; proteins
Subjects: calcium; copper; stress tolerance; stress alleviation; antioxidants; carotenoids; chlorophyll; glutathione; hydrogen peroxide; proline; proteins; wheat; phosphorus; potassium; magnesium; iron; boron; sodium
Species: Elodea canadensis

Received: January 8, 2012; Accepted: July 27, 2012; Published: June 1, 2013Show citation

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Min, H.L., Cai, S.J., Rui, Z., Sha, S., Xie, K.B., & Xu, Q.S. (2013). Calcium-mediated enhancement of copper tolerance in Elodea canadensis. Biologia plantarum, 57(2), 365-369. doi: 10.1007/s10535-012-0275-z.
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