Biologia plantarum 46:67-70, 2003 | DOI: 10.1023/A:1027380831429

Influence of a Brassinosteroid Analogue on Antioxidant Enzymes in Rice Grown in Culture Medium with NaCl

M. Núñez1, P. Mazzafera2, L.M. Mazorra1, W.J. Siqueira3, M.A.T. Zullo3
1 Departamento de Fisiología y Bioquímica Vegetal, Instituto Nacional de Ciencias Agrícolas, San José de las Lajas, La Habana, Cuba
2 Departamento de Fisiologia Vegetal, IB, Universidade de Campinas, Campinas, SP, Brasil
3 Centro de Recursos Genéticos Vegetais, Instituto Agronômico, Campinas, SP, Brasil

We studied the effects of a polyhydroxylated spirostanic brassinosteroid analogue (BB-16) on the activities of antioxidant enzymes in rice seedlings grown in vitro in culture medium supplemented with NaCl. Seedlings were grown in medium with 75 mM NaCl and 0.001 or 0.01 mg dm-3 BB-16 for 16 d or 3-d-old seedlings were exposed for 4 d to 0, 0.001 or 0.01 mg dm-3 BB-16 then further grown in medium with 75 mM NaCl without BB-16. Seedlings exposed to 0.01 mg dm-3 BB-16 for 16 d showed significant increase in the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR) and a slight increase in ascorbate peroxidase (APX). On the other hand, 4-d exposure to BB-16 only increased SOD and CAT activities at concentration 0.001 mg dm-3. GR activity was not altered by this BB-16 treatment. These results indicated that BB-16, which is structurally modified in the lateral chain in relation to natural brassinosteroids, changes the activity of key antioxidant enzymes, which might confer tolerance to saline stress.

Keywords: ascorbate peroxidase; catalase; glutathione reductase; Oryza sativa; saline stress; superoxide dismutase
Subjects: antioxidants; ascorbate peroxidase; brassinosteroid analogue, antioxidant enzymes; brassinosteroids; catalase; glutathione reductase; in vitro culture, NaCl stress; in vitro culture, nutrient media modification; Oryza sativa; peroxidases; reactive oxygen species (ROS); rice, antioxidant enzymes; salt stress, brassinosteroid analogue, antioxidant enzymes; superoxide dismutase

Published: July 1, 2003Show citation

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Núñez, M., Mazzafera, P., Mazorra, L.M., Siqueira, W.J., & Zullo, M.A.T. (2003). Influence of a Brassinosteroid Analogue on Antioxidant Enzymes in Rice Grown in Culture Medium with NaCl. Biologia plantarum, 46(1), 67-70. doi: 10.1023/A:1027380831429.
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