Biologia plantarum 57:267-273, 2013 | DOI: 10.1007/s10535-012-0292-y

Isolation and characterization of purple acid phosphatase gene during seedling development in mungbean

A. Wongkaew1,2, P. Srinives1,3, S. Nakasathien1,4,*
1 Center for Agricultural Biotechnology, Kasetsart University, Nakhon Pathom, Thailand
2 Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok, Thailand
3 Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, Thailand
4 Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand

Purple acid phosphatases (PAPs), which are normally found in plant tissues, can hydrolyze a broad spectrum of phosphate esters. In this study, a mungbean [Vigna radiata (L.) Wilczek cv. KPS1] acid phosphatase gene (VrPAP1) was isolated from seedling cotyledons. The full-length of VrPAP1 cDNA contained an open reading frame of 1 644 bp encoding 547 amino acid residues with a predicted molecular mass of 62.07 kDa. Sequence analysis showed that VrPAP1 is purple acid phosphatase. RNA blot analyses indicated that the VrPAP1 accumulated during the first hour in cotyledons of germinating seeds and reached a maximum expression after 24 h and then decreased. The VrPAP1 mRNA was observed in cotyledons, hypocotyls and leaves but not in radicles or dry seeds. DNA blot analysis indicated that VrPAP1 is a single copy gene in the mungbean genome.

Keywords: gene expression; RACE; RT-PCR; Vigna radiata
Subjects: purple acid phosphatase; seedling development; mungbean; gene expression; RACE; RT-PCR; amino acid sequences; phylogenetic tree
Species: Vigna rediata; Vigna radiata

Received: December 27, 2011; Accepted: August 10, 2012; Published: June 1, 2013Show citation

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Wongkaew, A., Srinives, P., & Nakasathien, S. (2013). Isolation and characterization of purple acid phosphatase gene during seedling development in mungbean. Biologia plantarum, 57(2), 267-273. doi: 10.1007/s10535-012-0292-y.
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