Biologia plantarum 2014, 58:201-208 | DOI: 10.1007/s10535-013-0388-z

Histological and biochemical parameters of Crocus sativus during in vitro root and shoot organogenesis

E. Vatankhah1,2,*, V. Niknam2, H. Ebrahimzadeh2
1 Department of Biology, Faculty of Science, University of Zanjan, Zanjan, I.R. Iran
2 Department of Plant Sciences, School of Biology, and Center of Excellence in Phylogeny of Living Organisms in Iran, College of Science, University of Tehran, Tehran, I.R. Iran

Content of malondialdehyde (MDA), proline, phenolics, and saccharides was analyzed during different developmental stages of in vitro root and shoot organogenesis in saffron. The highest content of MDA, proline, and phenolics was detected in nodular calli. Significant changes were also found in the content of polysaccharides, soluble saccharides, oligosaccharides, and reducing saccharides during developmental stages. Histological investigation of nodular calli showed meristematic zones with small and densely stained cells situated at peripheral zones of calli. The meristematic zones surrounded some vascular areas from which de novo organs originated. The parenchymatic cells of inner zones of calli converted to procambium cells that produced vascular tissues.

Keywords: lipid peroxidation; malondialdehyde; nodular callus; phenolics; proline; saccharides; saffron
Subjects: lipid peroxidation; malondialdehyde; callus; in vitro culture; phenolics; proline; saccharides; developmental stages; saffron
Species: Crocus sativus

Received: September 11, 2012; Revised: September 4, 2013; Accepted: September 6, 2013; Published: June 1, 2014Show citation

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Vatankhah, E., Niknam, V., & Ebrahimzadeh, H. (2014). Histological and biochemical parameters of Crocus sativus during in vitro root and shoot organogenesis. Biologia plantarum, 58(2), 201-208. doi: 10.1007/s10535-013-0388-z.
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