Biologia plantarum 45:11-17, 2002 | DOI: 10.1023/A:1015182608782

A New Somaclone of Prunus Avium Shows Diverse Growth Pattern under Different Spectral Quality of Radiation

C. Piagnani1, C. Iacona2, M.C. Intrieri3, R. Muleo4
1 Dipartimento di Produzione Vegetale, Milan University, Milan, Italy
2 Dipartimento di Coltivazione e Difesa delle Specie Legnose, Pisa University, Pisa, Italy
3 Dipartimento Biologia Animale Genetica, Florence University, Florence, Italy
4 Dipartimento Produzione Vegetale, Tuscia University, Viterbo, Italy

The aim of this research was to set up a regeneration protocol from mature explants of Prunus avium L. cv. Hedelfinger and to develop an early screening method for selection of putative somaclones based on morphological and physiological traits regulated by the spectral quality of radiation. DNA analyses of a new somaclone named HS, conducted using Inter Simple Sequence Repeat (ISSR), revealed a polymorphism between the somaclone HS and wild type propagated by microcuttings. When grown under different spectral quality of radiation, somaclone HS showed a different pattern of growth and development compared to the wild type with the main modifications related to apical dominance and chlorophyll production. Somaclone HS showed reduced apical dominance compared to the wild type. Wild type shoots grown in darkness showed chlorophyll a and b contents at levels in both cases comparable to those recorded under red radiation while HS did not retain the same capability.

Keywords: apical dominance; branching; cherry; chlorophyll; ISSR markers; polymorphism
Subjects: apical dominance, spectral quality of radiation; branching, spectral quality of radiation; cherry, spectral quality of radiation; chlorophyll, spectral quality of radiation; in vitro culture, nutrient media modification; Inter Simple Sequence Repeat (ISSR) markers; markers; polymorphism, proteins; proteins, polymorphism; Prunus avium; radiation spectral quality, growth; somaclone, new, cherry, spectral quality of radiation; spectral quality of radiation

Published: March 1, 2002Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Piagnani, C., Iacona, C., Intrieri, M.C., & Muleo, R. (2002). A New Somaclone of Prunus Avium Shows Diverse Growth Pattern under Different Spectral Quality of Radiation. Biologia plantarum, 45(1), 11-17. doi: 10.1023/A:1015182608782.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Adamson, H.Y., Hiller, R.G., Walmsley, J.: Protochlorophyllide reduction and greening in angiosperms: an evolutionary perspective.-J. Photochem. Photobiol. 41: 201-221, 1997. Go to original source...
    2. Ballaré, C.L., Scopel, A.L., Sanchez, R.A.: Foraging for light - photosensory ecology and agricultural implications.-Plant Cell Environ. 20: 820-825, 1997. Go to original source...
    3. Bourgin, J.P., Nitsch, J.P.: Obtention de Nicotiana aploïdes à partir d'étamines cultivées in vitro.-Ann. Physiol. vég. 9: 377-382, 1967.
    4. Caboni, E., Lauri, P., D'Angeli, S.: Somaclonal variation induced by adventitious shoot regeneration in pear and apple.-Acta Hort. 530: 195-199, 1999.
    5. De Salvador, F.R., Albertini, A., Buccheri, M.: Cherry: growth control and plantation system-In: Godini, A. (ed.).: Atti del "Convegno Nazionale del Ciliegio, Valenzano, 19-21 giugno 1997". Pp. 259-261. Istituto Agronomico Mediterraneo, Valenzano 1997.
    6. Demeasure, B., Sodzi, N., Petit, R.J.: A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in Plants.-Mol. Ecol. 4: 129-131, 1995. Go to original source...
    7. Donovan, A.M., Morgan, R., Valobra Piagnani, C., Ridout, M.S., James, D.J., Garrett, C.M.E.: Assessment of somaclonal variation in apple I: Resistance to the fire blight pathogen Erwinia amylovora.-J. hort. Sci. 69: 105-113, 1994. Go to original source...
    8. Godwin, I.D., Aitken, E.A.B., Smith, L.W.: Application of inter simple sequence repeat (ISSR) markers to plant genetics.-Electrophoresis 18: 1524-1528, 1997. Go to original source...
    9. Hammat, N., Grant, N.J.: Shoot regeneration from leaves of Prunus serotina Ehrl. (black cherry) and Prunus avium L. (wild cherry).-Plant Cell Rep. 17: 526-530, 1998. Go to original source...
    10. Hammatt, N., Blake, P.S., Hand, P.: Characterisation and use of apparent rejuvenation achieved during micropropagation of mature Prunus avium L.-In: Davey, M.R., Alderon, P.G., Lowe, K.C., Power, J.B. (ed.): Tree Biotechnology: Towards the Millennium. Pp. 45-63. Nottingham University Press, Nottingham 1998.
    11. Harding, K., Benson, E.E., Roubelakisangelakis, K.A.: Methylated DNA changes associated with the initiation and maintenance of Vitis vinifera in vitro shoot and callus cultures - a possible mechanism for age-related changes.-Vitis 35: 79-85, 1996.
    12. Kantety, R.V., Zeng, X.P., Bennetzen, J.L., Zehr, B.E.: Assessment of genetic diversity in dent and popcorn (Zea mays L.) inbred lines using inter-simple sequence repeat (ISSR) amplification.-Mol. Breed. 1: 365-373, 1995. Go to original source...
    13. Muleo, R., Boscherini, G., Bigozzi, L., Leva, A.R., Buiatti, M.: Differential appearance of specific RAPD polymorphic fragments in Actinidia plants regenerated on high osmotic and saline media.-Plant Physiol. Biochem. (Special Issue): 251, 1996a.
    14. Muleo, R., Casano, S., Bigozzi, L., Morini, S.: [The role of photoreceptors on plum Mr. S 2/5 in vitro shoot proliferation and rooting.]-In: Barbera, G., Barone, E., Sottile, F. (ed.): Atti delle "III Giornate Scientifiche S.O.I.", Erice. Pp. 341-342. Palermo 1996b. [In Ital.]
    15. Muleo, R., Thomas, B.: Effect of light quality on Prunus cerasifera in vitro are the result of differential effects on bud induction and apical dominance.-J. hort. Sci. 72: 483-491, 1997. Go to original source...
    16. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    17. Negri, P., Magnanini, E., Cantoni, L., Berardi, G., Sansavini, S.: [Transgenic trees: first experiments on transformation with the genes responsible for the control of vegetative habit.]-Riv. Frutticolt. 5: 91-97, 1998. [In Ital.]
    18. Parsons, B.J., Newbury, H.J., Jackson, M.T., Ford-Lloyd, B.V.: Contrasting genetic diversity relationships are revealed in rice (Oryza sativa L.) using different marker types.-Mol. Breed. 3: 115-125, 1997. Go to original source...
    19. Porra, R.J., Thompson, W.A., Kriedemann, P.E.: Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy.-Biochim. biophys. Acta 975: 384-394, 1989. Go to original source...
    20. Reddy, K.D., Nagaraju, J., Abraham, E.G.: Genetic characterisation of the silkworm Bombyx mori by simple sequence repeat (SSR)-anchored PCR.-Heredity 83: 681-687, 1999. Go to original source...
    21. Salimath, S.S., De Olivera, A.C., Godwin, I.D., Bennetzen, J.L.: Assesment of genome origins and genetic diversity in the genus Eleucine with DNA markers.-Genome 38: 757-763, 1995. Go to original source...
    22. Skirvin, M.R.: Natural and induced variation in tissue culture.-Euphytica 27: 241-266, 1978. Go to original source...
    23. Sul, W.I., Korban, S.S.: A highly efficient method for isolating genomic DNA from plant tissues.-Plant Tissue Cult. Biotechnol. 2: 113-116, 1996.
    24. Walmsley, J., Adamson, H.: Chlorophyll turnover in etiolated greening barley transferred to darkness: Isotopic (1-14C glutamic acid) evidence of dark chlorophyll synthesis in the absence of chlorophyll accumulation.-Physiol. Plant. 93: 435-444, 1994.
    25. Wang, G.J., Castiglione, S., Chen, Y., Li, L., Han, Y.F., Tian, YC., Gabriel, D.W., Han, Y.N., Mang, Q., Sala, F.: Poplar (Populus nigra L) plants transformed with a Bacillus thuringiensis toxin gene - insecticidal activity and genomic analysis.-Transgenic Res. 5: 289-301, 1996. Go to original source...
    26. Yang, H.Y., Schmidt, H., Kett, U.: Adventitious shoot regeneration in vitro in cherries. I. Adventitious shoot formation from in vitro-cultured leaves of the cherry rootstock '209/1'.-Gartenbauwissenschaft 56: 210-213, 1991.
    27. Zietkienicz, E., Rafalksi, A., Labuda, D.: Genome fingerprinting by simple sequence repeats (SSR) anchored polymerase chain reaction amplification.-Genomics 20: 176-183, 1994. Go to original source...

    Return to the content