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Publications
All publications![Squaramate‐Modified Nucleotides and DNA for Specific Cross‐Linking with Lysine‐Containing Peptides and Proteins](https://webarchiv.lib.cas.cz:443/wayback/20191003063500im_/https://www.uochb.cz/publication/article/articles12288.jpeg?v=1570077226)
Squaramate‐Modified Nucleotides and DNA for Specific Cross‐Linking with Lysine‐Containing Peptides and Proteins
Angewandte Chemie International Edition 58 (38): 13345-13348 (2019).
Squaramate‐linked 2′‐deoxycytidine 5′‐O‐triphosphate was synthesized and found to be good substrate for KOD XL DNA polymerase in primer extension or PCR synthesis of modified DNA. The resulting squaramate‐linked DNA reacts with primary amines to form a stable diamide linkage. This reaction was used for bioconjugations of DNA with Cy5 and Lys‐containing peptides. Squaramate‐linked DNA formed covalent cross‐links with histone proteins. This reactive nucleotide has potential for other bioconjugations of nucleic acids with amines, peptides or proteins without need of any external reagent.
Oxidation of the B12 and CB11 icosahedral anions
Handbook of Boron Science: With Applications in Organometallics, Catalysis, Materials and Medicine, Volume 3: Boron in Materials 2019 : 137-158 (2019).
Metabolomics Based on MS in Mice with Diet-Induced Obesity and Type 2 Diabetes Mellitus: the Effect of Vildagliptin, Metformin, and Their Combination
Applied Biochemistry and Biotechnology 188 (1): 165-184 (2019).