Photosynthetica 2015, 53(4):613-616 | DOI: 10.1007/s11099-015-0138-x

Photosynthesis parameters during acclimatization of in vitro-grown olive plantlets

A. Chaari-Rkhis1,*, M. Maalej2, A. Chelli-Chaabouni3, L. Fki2, N. Drira2
1 Olive Institute, University of Sfax, Sfax, Tunisia
2 Faculty of Science, University of Sfax, Sfax, Tunisia
3 National Institute of Agronomic Research, University of Carthage, Ariana, Tunisia

Monitoring some parameters would help to overcome the difficulties that can affect in vitro-grown plants during the crucial step of their acclimatization. Thus, after the determination of net photosynthesis and other parameters during acclimatization of in vitro-grown olive plantlets, we concluded that three months after the transfer to ex vitro, the in vitro-grown olive plants become well acclimated. In fact, even though the net photosynthesis, relatively high in vitro, recorded low values after 15 d from the transfer, it reverted back to its standard rates after 180 d of acclimatization. Transpiration and stomatal conductance first increased significantly with a maximum of 6.22 mmol(H2O) m-2 s-1 and 1.8 mmol(H2O) m-2 s-1, respectively, but they regressed to very low values after 180 d of acclimatization. Some changes in the leaf anatomy were also observed; the reduction of stomata density and inversely, the increase of trichome density, especially on the abaxial side of the leaves, were observed.

Keywords: gas exchange; in vitro culture; micropropagation; Olea

Received: August 15, 2014; Accepted: February 6, 2015; Published: December 1, 2015Show citation

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Chaari-Rkhis, A., Maalej, M., Chelli-Chaabouni, A., Fki, L., & Drira, N. (2015). Photosynthesis parameters during acclimatization of in vitro-grown olive plantlets. Photosynthetica, 53(4), 613-616. doi: 10.1007/s11099-015-0138-x.
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