Photosynthetica 2010, 48(4):610-616 | DOI: 10.1007/s11099-010-0078-4

The effect of amphiphilic peptide surfactants on the light-harvesting complex II

S. Liu1,*, Y. Qiu1, D. Y. Yu1
1 Center for Bioengineering and Biotechnology, China University of Petroleum, Qingdao, China

The peptide surfactants are amphiphilic peptides which have a hydrophobic tail and a hydrophilic head, and have been reported to stabilize and protect some membrane proteins more effectively than conventional surfactants. The effects of a class of peptide surfactants on the structure and thermal stability of the photosynthetic membrane protein lightharvesting complex II (LHCII) in aqueous media have been investigated. After treatment with the cationic peptide surfactants A6K, V6K2, I5K2 and I5R2, the absorption at 436 nm and 470 nm decreased and the absorption at 500-510 nm and 684-690 nm increased. Moreover, the circular dichroism (CD) signal intensity in the Soret region also decreased significantly, indicating the conformation of some chlorophyll (Chl) a, Chl b, and the xanthophyll molecules distorted upon cationic peptide surfactants treatment. The anionic peptide surfactants A6D and V6D2 had no obvious effect on the absorption and CD spectra. Except for A6D, these peptides all decreased the thermal stability of LHCII, indicating that these peptides may reconstitute protein into a less stable conformation. In addition, the cationic peptide surfactants resulted in LHCII aggregation, as shown by sucrose gradient ultracentrifugation and fluorescence spectra.

Keywords: circular dichroism; light-harvesting complex II; peptide surfactants; thermal stability

Received: December 20, 2009; Accepted: October 28, 2010; Published: December 1, 2010Show citation

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Liu, S., Qiu, Y., & Yu, D.Y. (2010). The effect of amphiphilic peptide surfactants on the light-harvesting complex II. Photosynthetica, 48(4), 610-616. doi: 10.1007/s11099-010-0078-4.
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