Photosynthetica 2006, 44(4):591-598 | DOI: 10.1007/s11099-006-0076-8

Developmental changes of plant affecting primary photosynthate distribution in rice leaves

T. Shinano1,*, K. Ando2, K. Okazaki3, M. Osaki2
1 Creative Research Initiative "Sousei" (CRIS), Hokkaido University, Sapporo, Japan
2 Graduate School of Agriculture, Hokkaido University, Sapporo, Japan
3 National Agricultural Research Center for Hokkaido Region, Sapporo, Japan

Developmental changes of plant in the regulation of photosynthate distribution of leaves were studied in hydroponically cultivated rice by the 14CO2 tracer technique and analysis of the activity of the regulatory enzymes, sucrose phosphate synthase (SPS), phosphoenolpyruvate carboxylase (PEPC), and pyruvate kinase (PK). The distribution of primary photosynthates into sugars, amino acids, organic acids, sugar phosphates, proteins, and polysaccharides was determined by column chromatography. The relative primary photosynthate distribution to the sugar phosphate fraction was significantly larger in the 5th leaf than in the 6th one. Correspondingly, the Vmax of PEPC was significantly higher in the 5th than in the 6th leaf, while no significant differences between leaves were detected in the other enzymes. As a consequence, the ratio of the Vmax of SPS and PEPC was lower in the 5th than in the 6th leaf. As the 5th leaf develops before panicle initiation in rice, it predominantly supports vegetative growth, while the 6th leaf develops after panicle initiation and thus contributes mainly to reproductive growth. We conclude that the physiological properties of each leaf are regulated developmentally. When the 6th leaf became fully expanded (corresponding to the panicle initiation stage of plant), the distribution pattern of 14C was transiently changed in the 5th leaf, indicating that individual organs that are mainly involved in vegetative development are affected to some extent by the whole-plant-level physiological transformation that occurs at the transition from the vegetative to the reproductive stage.

Keywords: 14CO2; leaf position; Oryza sativa; phosphoenolpyruvate carboxylase; primary photosynthate; pyruvate kinase; sucrose phosphate synthase

Received: February 2, 2006; Accepted: April 20, 2006; Published: December 1, 2006Show citation

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Shinano, T., Ando, K., Okazaki, K., & Osaki, M. (2006). Developmental changes of plant affecting primary photosynthate distribution in rice leaves. Photosynthetica, 44(4), 591-598. doi: 10.1007/s11099-006-0076-8.
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    References

    1. Baxter, C.J., Foyer, C.H., Turner, J., Rolfe, S.A., Quick, W.P.: Elevated sucrose-phosphate synthase activity in transgenic tobacco sustains photosynthesis in older leaves and alters development.-J. exp. Bot. 54: 1813-1820, 2003.
    2. Bradford, M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    3. Champigny, M.-L., Foyer, C.: Nitrate activation of cytosolic protein kinases diverts photosynthetic carbon from sucrose to amino acid biosynthesis. Basis for a new concept.-Plant Physiol. 100: 7-12, 1992. Go to original source...
    4. Duff, S.M.G., Chollet, R.: In vivo regulation of wheat-leaf phosphoenolpyruvate carboxylase by reversible phosphorylation.-Plant Physiol. 107: 775-782, 1995. Go to original source...
    5. Foyer, C.H., Noctor, G., Lelandais, M., Lescure, J.C., Valadier, M.H., Boutin, J.P., Horton, P.: Short-term effects of nitrate, nitrite and ammonium assimilation on photosynthesis, carbon partitioning and protein phosphorylation in maize.-Planta 192: 211-220, 1994. Go to original source...
    6. Halford, N.G., Paul, M.J.: Carbon metabolite sensing and signaling.-Plant Biotech. J. 1: 381-398, 2003. Go to original source...
    7. Hind, G.: Thylakoid components and processes.-In: Hall, D.O., Scurlock, J.M.O., Bolhàr-Nordenkampf, H.R., Leegood, R.C., Long, S.P. (ed.): Photosynthesis and Production in a Changing Environment. A Field and Laboratory Manual. Pp. 283-298. Chapman & Hall, London-Glasgow-New York-Tokyo-Melbourne-Madras 1993.
    8. Huber, J.L., Redinbaugh, M.G., Huber, S.C., Campbell, W.H.: Regulation of maize leaf nitrate reductase activity involves both gene expression and protein phosphorylation.-Plant Physiol. 106: 1667-1674, 1994.
    9. Huber, S.C., Nielsen, T.H., Huber, J.L.A., Pharr, D.M.: Variation among species in light activation of sucrosephosphate synthase.-Plant Cell Physiol. 30: 277-285, 1989. Go to original source...
    10. Laporte, M.M., Galagan, J.A., Prasch, A.L., Vanderveer, P.J., Hanson, D.T., Shewmaker, C.K., Sherkey, T.D.: Promoter strength and tissue specificity effects on growth of tomato plants transformed with maize sucrose-phosphate synthase.-Planta 212: 817-822, 2001. Go to original source...
    11. Laporte, M.M., Galagan, J.A., Shapiro, J.A., Boersig, M.R., Shewmaker, C.K.: Sucrose-phosphate synthase activity and yield analysis of tomato plants transformed with maize sucrose-phosphate synthase.-Planta 203: 253-259, 1997. Go to original source...
    12. Li, B., Zhang, X.Q., Chollet, R.: Phosphoenolpyruvate carboxylase kinase in tobacco leaves in activated by light in a similar but not identical way as in maize.-Plant Physiol. 111: 497-505, 1996. Go to original source...
    13. Mahn, C.T., Bismuth, E., Boutin, J.P., Provot, M., Champigny, M.L.: Metabolite effectors for short-term nitrogen-dependent enhancement of phosphoenolpyruvate carboxylase activity and decrease of net sucrose synthesis in wheat leaves.-Physiol. Plant. 89: 460-466, 1993.
    14. Mizuno, N., Minami, M.: [The use of H2SO4-H2O2 for the destruction of plants matter as a preliminary to determination of N, K, Mg, Ca, Fe, Mn.]-Jap. J. Soil Sci. Plant Nutr. 51: 418-420, 1980. [In Jap.]
    15. Murchie, E.H., Sarrobert, C., Contard, P., Betsche, T., Foyer, C., Galtier, N.: Overexpression of sucrose-phosphate synthase in tomato plants grown with CO2 enrichment leads to decreased controls.-Plant Physiol. Biochem. 37: 251-260, 1999. Go to original source...
    16. Nakamura, T., Osaki, M., Shinano, T., Tadano, T.: Difference in system of current photosynthesized carbon distribution to carbon and nitrogen compounds between rice and soybean.-Soil Sci. Plant Nutr. 43: 777-788, 1997. Go to original source...
    17. Ohto, M., Onai, K., Furukawa, Y., Aoki, E., Araki, T., Nakamura, K.: Effects of sugar on vegetative development and floral transition in Arabidopsis.-Plant Physiol. 127: 252-261, 2001. Go to original source...
    18. Osaki, M.: Ontogenic changes of N, P, and K contents in individual leaves of field crops.-Soil Sci. Plant Nutr. 41: 429-438, 1995. Go to original source...
    19. Osaki, M., Hada, K., Tanaka, A.: [Reconstruction of the leafproteins into grain-proteins during ripening in the rice plant.]-Jap. J. Soil Sci. Plant Nutr. 59: 272-278, 1988. [In Jap.]
    20. Osaki, M., Shinano, T., Tadano, T.: Redistribution of carbon and nitrogen compounds from the shoot to the harvesting organs during maturation in field crops.-Soil Sci. Plant Nutr. 37: 117-128, 1991. Go to original source...
    21. Plaxton, W.C.: Metabolic aspects of phosphate starvation in plants.-In: Lynch, J.P., Deikman, J. (ed.): Phosphorus in Plant Biology: Regulatory Roles in Molecular, Cellular, Organismic, and Ecosystem Processes. Pp. 41-93. American Society of Plant Physiologists, Rockville 1998.
    22. Quy, L.V., Champigny, M.L.: NO3 - enhances the kinase activity for phosphorylation of phosphoenolpyruvate carboxylase and sucrose phosphate synthase proteins in wheat leaves.-Plant Physiol. 99: 344-347, 1992.
    23. Quy, L.V., Foyer, H., Champigny, M.L.: Effect of light and NO3 - on wheat leaf phosphoenolpyruvate carboxylase activity.-Plant Physiol. 97: 1476-1482, 1991.
    24. Redgwell, R.J.: Fractionation of plant extracts using ion-exchange Sephadex.-Anal. Biochem. 107: 44-50, 1980. Go to original source...
    25. Sheible, W.R., Gonzalez-Fontes, A., Lauerer, M., Muller-Rober, B., Caboche, M., Stitt, M.: Nitrate acts as a signal to induce organic acid metabolism and repress starch metabolism in tobacco.-Plant Cell 9: 783-798, 1997. Go to original source...
    26. Shinano, T., Nakajima, K., Wasaki, J., Mori, H., Zheng, T., Osaki, M.: Developmental regulation of photosynthate distribution in leaves of rice.-Photosynthetica 44: 1-10, 2006. Go to original source...
    27. Shinano, T., Nanamori, M., Dohi, M., Wasaki, J., Osaki, M.: Evaluation of phosphorus starvation inducible genes relating to efficient phosphorus utilization in rice.-Plant Soil 269: 81-87, 2005. Go to original source...
    28. Shinano, T., Osaki, M., Tadano, T.: 14C-allocation of 14C-compounds introduced to a leaf to carbon and nitrogen components in rice and soybean during ripening.-Soil Sci. Plant Nutr. 40: 199-209, 1994. Go to original source...
    29. Signora, L., Galtier, N., Skøt, L., Lucas, H., Foyer, C.H.: Overexpression of sucrose phosphate synthase in Arabidopsis thaliana results in increased foliar sucrose/starch ratios and favours decreased foliar carbohydrate accumulation in plants after prolonged growth with CO2 enrichment.-J. exp. Bot. 49: 669-680, 1998.
    30. Stitt, M.: Nitrate regulation of metabolism and growth.-Curr. Opin. Plant Biol. 2: 178-186, 1999. Go to original source...
    31. Takahashi, S., Ono, K., Ugaki, M., Ishimaru, K., Aoki, N., Ohsugi, R.: Ser162-dependent inactivation of overproduced sucrose-phosphate synthase protein of maize leaf in transgenic rice plants.-Plant Cell Environ. 41: 977-981, 2000. Go to original source...
    32. Tanaka, A.: Studies on the nutrio-physiology of leaves of rice plant.-J. Fac. Agr. Hokkaido Univ. 51: 491-550, 1961.
    33. Worrel, A.C., Brunearu, J.-M., Summerfelt, K., Boersig, M., Voelker, T.A.: Expression of a maize sucrose phosphate synthase in tomato alters leaf carbohydrate partitioning.-Plant Cell 3: 1121-1130, 1991. Go to original source...

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