Photosynthetica 2014, 52(1):105-116 | DOI: 10.1007/s11099-014-0012-2

Response of photosynthesis and chlorophyll fluorescence to acute ozone stress in tomato (Solanum lycopersicum Mill.)

A. A. Thwe1,5,*, G. Vercambre2, H. Gautier2, F. Gay3, J. Phattaralerphong4, P. Kasemsap5
1 Department of Horticulture, Yezin Agricultural University, Nay Pyi Taw, Myanmar
2 INRA, UR 1115, Plantes et Systèmes de culture Horticoles, Avignon, Cedex 9, France
3 CIRAD, UMR111 Eco&Sols, Montpellier, France
4 Faculty of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Bangkok, Thailand
5 Department of Horticulture, Tropical Agriculture, Kasetsart University, Bangkok, Thailand

The crop sensitivity to ozone (O3) is affected by the timing of the O3 exposure, by the O3 concentration, and by the crop age. To determine the physiological response to the acute ozone stress, tomato plants were exposed to O3 at two growth stages. In Experiment I (Exp. I), O3 (500 μg m-3) was applied to 30-d-old plants (PL30). In Experiment II (Exp. II), three O3 concentrations (200, 350, and 500 μg m-3) were applied to 51-d-old plants (PL51). The time of the treatment was 4 h (7:30-11:30 h). Photosynthesis and chlorophyll fluorescence measurements were done 4 times (before the exposure; 20 min, 20 h, and 2-3 weeks after the end of the treatment) using a LI-COR 6400 photosynthesis meter. The stomatal pore area and stomatal conductance were reduced as the O3 concentration increased. Ozone induced the decrease in the photosynthetic parameters of tomato regardless of the plant age. Both the photosystem (PS) II operating efficiency and the maximum quantum efficiency of PSII photochemistry declined under the ozone stress suggesting that the PSII activity was inhibited by O3. The impaired PSII contributed to the reduced photosynthetic rate. The greater decline of photosynthetic parameters was found in the PL30 compared with the PL51. It proved the age-dependent ozone sensitivity of tomato, where the younger plants were more vulnerable. Ozone caused the degradation of photosynthetic apparatus, which affected the photosynthesis of tomato plants depending on the growth stage and the O3 concentration.

Keywords: tomato; spinach; sweet corn; chlorophyll fluorescence; ozone; stomatal pore area; stomatal conductance; photosystem II; maximum quantum efficiency of PSII photochemistry; electron transport rate; maximum rate of carboxylation; light-saturated photosynthetic rate; net photosynthetic rate; photosynthetic photon flux density; photochemical quenching coefficient; dark-respiration rate; Rubisco; ribulose-1; 5-bisphosphate carboxylase, oxygenase; triose phosphate use; maximum carboxylation rate of Rubisco; initial slope of the light curve; curve convexity; quantum yield of carboxylation rate; effective quantum yield of photosystem II photochemistry; PN-Ci curves; light-response curve; exponential decay function; nonrectangular hyperbola

Received: August 30, 2012; Accepted: July 1, 2013; Published: March 1, 2014Show citation

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Thwe, A.A., Vercambre, G., Gautier, H., Gay, F., Phattaralerphong, J., & Kasemsap, P. (2014). Response of photosynthesis and chlorophyll fluorescence to acute ozone stress in tomato (Solanum lycopersicum Mill.). Photosynthetica, 52(1), 105-116. doi: 10.1007/s11099-014-0012-2.
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