Photosynthetica 2010, 48(2):269-277 | DOI: 10.1007/s11099-010-0033-4

Effects of realistically simulated, elevated UV irradiation on photosynthesis and pigment composition of the alpine snow alga Chlamydomonas nivalis and the arctic soil alga Tetracystis sp. (Chlorophyceae)

D. Remias1,*, A. Albert2, C. Lütz1
1 Institute of Botany, University of Innsbruck, Innsbruck, Austria
2 Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany

An indoor sun simulator was used to provide elevated UV-B radiation (280-315 nm) in combination with realistic ratios to PAR (400-700 nm) and UV-A radiation (315-400 nm) in order to test the physiological response of a soil- and snow microalga during a three-day stress scenario, which may occasionally occur in their respective arctic and alpine habitats.
Chlamydomonas nivalis and Tetracystis sp. are initial colonizers of harsh habitats like summer snow fields and bare arctic soils. The two species were chosen because of their role as primary successors in places where life is generally limited by extreme climatic and nutritional conditions.
The influence of the increased UV-B irradiation (1.43 W m-2; control: 0.52 W m-2) on photosynthesis and pigment composition was measured. Both species survived this incubation without any morphological signs of damage, but oxygen production was reduced by 20-56%. Under control conditions, the amount of chlorophylls (Chls) and carotenoids (Cars) per dry mass increased after three days due to optimal light conditions. After the same period, the treated samples of the soil alga Tetracystis sp. showed a smaller increase in Chls and primary Cars than the control. However, the production of extraplastidal, secondary Cars was induced. On the contrary, the snow alga C. nivalis already had high amounts of secondary Cars before the experiment, and after exposure, all pigment classes increased more compared to control conditions. The results show that these microalgae can tolerate short episodes of enhanced UV-B radiation. Photosynthesis may be temporally impaired, but the cells respond by the production of secondary Cars, which can shield their chloroplasts against excessive irradiation or quench reactive oxygen species.

Keywords: microalgae; secondary carotenoids; sun simulator; UV-B exposure

Received: December 10, 2009; Accepted: April 28, 2010; Published: June 1, 2010Show citation

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Remias, D., Albert, A., & Lütz, C. (2010). Effects of realistically simulated, elevated UV irradiation on photosynthesis and pigment composition of the alpine snow alga Chlamydomonas nivalis and the arctic soil alga Tetracystis sp. (Chlorophyceae). Photosynthetica, 48(2), 269-277. doi: 10.1007/s11099-010-0033-4.
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    References

    1. Bidigare, R.R., Ondrusek, M.E., Kennicutt II, M.C., Iturriaga, R., Harvey., H.R., Hoham, R.W., Macko, S.A.: Evidence for a photoprotective function for secondary carotenoids of snow algae. - J. Phycol. 29: 427-434, 1993. Go to original source...
    2. Björn, L.O.: Photobiology - The Science of Life and Light. 2ed. Springer, NewYork 2008.
    3. Blumthaler, M.: Factors, trends and scenarios of UV radiation in arctic-alpine environments. - In: Ørbaek, J.B., Kallenborn, R., Tombre, I., Hegseth, E.N., Falk-Petersen, S., Hoel, A.H. (ed.): Arctic Alpine Ecosystems and People in a Changing Environment. Pp. 181-192. Springer, Berlin - Heidelberg 2007. Go to original source...
    4. Breen, K., Lévesque, E.: The influence of biological soil Crusts on soil characteristics along a High Arctic glacier foreland, Nunavut, Canada. - Arct. Antarc. Alp. Res. 40: 287-297, 2008. Go to original source...
    5. Büdel, B.: Microorganisms in Soils: Roles in genesis and functions. - In: Buscot, F., Varma, A. (ed.): Soil Biology, Vol. 3: Pp. 307-324. Springer, Berlin - Heidelberg, 2005.
    6. Caldwell, M.M.: Solar UV irradiation and the growth and development of higher plants. - In: Giese, A.C. (ed.): Photophysiology, Vol. VI. Pp. 131-177. Academic Press, New York - London 1971. Go to original source...
    7. Crawford, R.M.M.: Plants at the Margin: Ecological Limits and Climate Change. - Cambridge Univ. Press, New York 2008. Go to original source...
    8. Döhring, T., Köfferlein, M., Thiel, S., Seidlitz, H.K.: Spectral shaping of artificial UV-B irradiation for vegetation stress research. - J. Plant Physiol. 148: 115-119, 1996. Go to original source...
    9. Duval, B., Shetty, K., Thomas, W.H.: Phenolic compounds and antioxidant properties in the snow alga Chlamydomonas nivalis after exposure to UV light. - J. App. Phycol. 11: 559-566, 1999. Go to original source...
    10. Ettl, H.: Chlorophyta I. Phytomonadina. - In: Ettl, H., Gerloff, J., Heynig, H., Mollenhauer, D.: Süßwasserflora von Mitteleuropa 9. Fischer-Verlag, Jena 1983.
    11. Ettl, H., Gärtner, G.: Chlorophyta II: Tetrasporales, Chlorococcales, Gloaodendrales. - In: Ettl, H., Gerloff, J., Heynig, H., Mollenhauer, D.: Süßwasserflora von Mitteleuropa 10. Fischer-Verlag, Jena 1988.
    12. Gorton, H.L., Vogelmann, T.C.: Ultraviolet radiation and the snow alga Chlamydomonas nivalis (Bauer) Wille. - Photochem. Photobiol. 77: 608-615, 2003. Go to original source...
    13. Götz, M, Albert, A., Stich, S., Heller, W., Scherb, H., Krins, A., Langebartels, C., Seidlitz, H.K, Ernst, D.: PAR modulation of the UV-dependent levels of flavonoid metabolites in Arabidopsis thaliana (L.) Heynh. leaf rosettes: cumulative effects after a whole vegetative growth period. - Protoplasma (in press), 2010. early online: DOI 10.1007/s00709-009-0064-5 Go to original source...
    14. Heo, S.-J., Jeon, Y.-J.: Protective effect of fucoxanthin isolated from Sargassum siliquastrum on UV-B induced cell damage. - J. Photochem. Photobiol. B 95: 101-107, 2009. Go to original source...
    15. Hoham, R.W., Duval, B.: Microbial ecology of snow and freshwater ice with emphasis on snow algae. - In: Jones, H.G., Pomeroy, J.W., Walker, D.A., Hoham, R.W. (ed.): Snow Ecology. Pp. 168-228. Cambridge Univ. Press, New York 2001.
    16. Holzinger, A., Karsten, U., Lütz, C., Wiencke C.: Ultrastructure and photosynthesis in the supralittoral green macroalga Prasiola crispa from Spitsbergen (Norway) under UV exposure. - Phycologia 45: 168-177, 2006. Go to original source...
    17. Holzinger, A., Lütz, C.: Algae and UV irradiation: Effects on ultrastructure and related metabolic functions. - Micron 37: 190-207, 2006. Go to original source...
    18. Karsten, U., Lembcke, S., Schumann, R.: The effects of ultraviolet radiation on photosynthetic performance, growth and sunscreen compounds in aeroterrestrial biofilm algae isolated from building facades. - Planta 225: 991-1000, 2007. Go to original source...
    19. Kaštovská, K., Elster, J., Stibal, M., Šantrůčková, H.: Microbial Assemblages in soil microbial succession after glacial retreat in Svalbard (High Arctic). - Microb. Ecol. 50: 396-407, 2005. Go to original source...
    20. Leya, T.: Feldstudien und genetische Untersuchungen zur Kryophilie der Schneealgen Nordwestspitzbergens. - Shaker, Aachen 2004.
    21. Leya, T., Müller, T., Ling, H.U., Fuhr, G.: Taxonomy and Biophysical Properties of Cryophilic Microalgae and Their Environmental Factors in Northwest Spitsbergen, Svalbard. 57th Eastern Snow Conference, Syracuse. New York 2000.
    22. Leya, T., Rahn, A., Lütz, C., Remias, D.: Response of arctic snow and permafrost algae to high light and nitrogen stress by changes in pigment composition and applied aspects for biotechnology. - FEMS Microbiol. Ecol. 67: 432-443, 2009. Go to original source...
    23. Lütz, C., Blassnig, M., Remias, D.: Different flavonoid patterns in Deschampsia antarctica and Colobanthus quitensis from the maritime Antarctic. - In: Wiencke, C., Ferreyra, G.A., Abele, D., Marensii S. (ed.): Reports on Polar and Marine Research 571. Pp. 192-199. Alfred-Wegener-Institut, Bremerhaven 2008.
    24. Lütz, C., Seidlitz, H.K., Meindl, U.: Physiological and structural changes in the chloroplast of the green alga Micrasterias denticulata induced by UV-B simulation. - Plant Ecol. 128:54-64, 1997. Go to original source...
    25. Lütz-Meindl, U., Lütz, C.: Analysis of element accumulation in cell wall attached and intracellular particles of snow algae by EELS and ESI. - Micron 37: 452-458, 2006. Go to original source...
    26. MacIntyre, H.L., Kana, T.M., Anning, T., Geider, R.J.: Photoacclimation of photosynthesis irradiance responde curves and photosynthetic pigments in microalgae and cyanobacteria. - J. Phycol. 38: 17-38, 2002. Go to original source...
    27. Marshall, W.A., Chalmers, M.O.: Airborne dispersal of antarctic terrestrial algae and cyanobacteria. - Ecography 20:585-594, 1997. Go to original source...
    28. Meindl, U., Lütz, C.: Effects of UV irradiation on cell development and ultrastructure of the green alga Micrasterias. - J. Photochem. Photobiol. 36: 285-292, 1996. Go to original source...
    29. Morgan-Kiss, R.M., Priscu, J.C., Pocock, T., Gudynaite-Savitch, L., Huner, N.P.A.: Adaptation and Acclimation of Photosynthetic Microorganisms of Permanently Cold Environments. - Microbiol. Mol. Biol. Rev. 70: 222-252, 2006. Go to original source...
    30. Müller, T., Leya, T., Fuhr, G.: Persistent snow algal fields in Spitsbergen: Field observations and a hypothesis about the annual cell circulation. - Arct. Antarct. Alp. Res. 33: 42-51, 2001. Go to original source...
    31. Patova, E.N., Dorokhova, M.F.: Green Algae in tundra soils affected by coal mine pollutions. - Biologia 63: 831-835, 2008. Go to original source...
    32. Porra, R.J., Thomson, W.A., Kreidmann, P.E.: Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by absorption spectroscopy. - Biochim. Biophys. Acta 975: 384-394, 1989. Go to original source...
    33. Reisser, W., Houben, P.: Different strategies of aeroterrestrial algae in reacting to increased levels of UV-B and ozone. - Nova Hedwigia 123: 291-296, 2001.
    34. Remias, D., Holzinger, A., Lütz, C.: Physiology, ultrastructure and habitat of the ice alga Mesotaenium berggrenii (Zygnemaphyceae, Chlorophyta) from glaciers in the European Alps. - Phycologia 48: 302-312, 2009. Go to original source...
    35. Remias, D., Lütz-Meindl, U., Lütz, C.: Photosynthesis, pigments and ultrastructure of the alpine snow alga Chlamydomonas nivalis. - Eur. J. Phycol. 40: 259-268, 2005. Go to original source...
    36. Remias, D., Lütz, C.: Characterisation of esterified secondary carotenoids and of their isomers in green algae: a HPLC approach. - Algol. Studies 124: 85-94, 2007. Go to original source...
    37. Rozema, J., Boelen, P., Blokker, P.: Depletion of stratospheric ozone over the Antarctic and Arctic: Responses of plants of polar terrestrial ecosystems to enhanced UV-B, an overview. - Environ. Pollut. 137: 428-442, 2005. Go to original source...
    38. Shick, J.M., Dunlap, W.C.: Mycosporine-like amino acids and related gadusols: Biosynthesis, accumulation, and UVprotective functions in aquatic organisms. - Annu. Rev. Physiol. 64: 223-262, 2002. Go to original source...
    39. Stibal, M., Elster, J., Šabacká, M., Kaštovská, K.: Seasonal and diel changes in photosynthetic activity of the snow alga Chlamydomonas nivalis (Chlorophyceae) from Svalbard determined by pulse amplitude modulation fluorometry. - FEMS Microbiol. Ecol. 59: 265-273, 2007. Go to original source...
    40. Sommaruga, R., Garcia-Pichel, F.: UV-absorbing mycosporinelike compounds in planctonic and benthic organisms from a high-mountain lake. - Arch. Hydrobiol. 144: 255-269, 1999. Go to original source...
    41. Teranishi, M., Nakamura, K., Morioka, H., Yamamoto, K., Hidema, J.: The native cyclobutane pyrimidine dimer photolyase of rice is phosphorylated. - Plant Physiol. 146: 1941-1951, 2008.
    42. Thiel, S., Döhring, T., Köfferlein, M., Kosak, A., Martin, P., Seidlitz, H.K.: A phytotron for plant stress research: How far can artificial lighting compare to natural sunlight? - J. Plant Physiol. 148: 456-463, 1996. Go to original source...
    43. Thomas, W.H., Duval, B.: Sierra Nevada, California, U.S.A., snow algae: Snow albedo changes, algal-bacterial interrelationships, and ultraviolet radiation effects. - Arc. Alp. Res. 27: 389-399, 1995. Go to original source...
    44. Xiong, F., Nedbal, L., Neori, A.: Assessment of UV-B sensitivity of photosynthetic apparatus among microalgae: Short-term laboratory screening versus long-term outdoor exposure. - J. Plant Physiol. 155: 54-62, 1999. Go to original source...
    45. Yang, Y.Q., Yao, Y.: Photosynthetic responses to solar UV-A and UV-B radiation of low- and high-altitude populations of Hippophae rhamnoides. - Photosynthetica 46: 307-311, 2008. Go to original source...

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