Photosynthetica 2012, 50(3):467-471 | DOI: 10.1007/s11099-012-0042-6

Black leaf-clips of a commercial fluorometer increased leaf temperature during dark adaptation under high solar radiation

P. Giorio1,*, V. Nuzzo2, G. Guida1, R. Albrizio1
1 National Research Council of Italy, Institute for Agricultural and Forestry Systems in the Mediterranean (CNR-I.S.A.FO.M.), Ercolano (NA), Italy
2 Department of Crop Systems, Forestry and Environmental Science, University of Basilicata, Potenza, Italy

The use of black leaf-clips for dark adaptation under high solar radiation conditions is reported to underestimate the maximum quantum yield of PSII photochemistry (Fv/Fm) measured by the continuous-excitation fluorometer Pocket PEA. The decrease in Fv/Fm was due to a rise in minimum fluorescence emission (Fo), probably resulting from increased leaf temperature (Tl). In field-grown tomato and pepper, fluorescence parameters and Tl in the region covered by the black leaf clip were measured in clipped leaves exposed to solar radiation during dark adaptation (clipped-only leaves) and in clipped leaves protected from solar radiation by aluminium foil (shrouded clipped leaves). Results confirmed significant Fv/Fm underestimates in clipped-only leaves primarily due to increased Fo. In one tomato experiment, Tl increased from 30 to 44.5°C in clipped-only leaves, with a negligible rise in shrouded clipped leaves. In two respective pepper experiments, Tl in clipped-only leaves increased from 27 to 36.2°C and 33 to 40.9°C. Based on the results of this study, a clip-effect parameter (PCE) on fluorescence emission is proposed as the difference for Fv/Fm (or -Fo/Fm) between shrouded clipped leaves and clipped-only leaves, which resulted to be 0.706 for tomato, and 0.241 and 0.358 for the two pepper experiments.

Keywords: leaf-clip effect; leaf temperature; parameter; pepper; photochemical efficiency; tomato

Received: October 18, 2011; Accepted: March 29, 2012; Published: September 1, 2012Show citation

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Giorio, P., Nuzzo, V., Guida, G., & Albrizio, R. (2012). Black leaf-clips of a commercial fluorometer increased leaf temperature during dark adaptation under high solar radiation. Photosynthetica, 50(3), 467-471. doi: 10.1007/s11099-012-0042-6.
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