Photosynthetica 2018, 56(4):1140-1146 | DOI: 10.1007/s11099-018-0798-4

Physio-anatomical responses of tobacco under caffeine stress

R. Alkhatib1,*, B. Alkhatib2, L. Al-Eitan2, N. Abdo3, M. Tadros4, E. Bsoul5
1 Department of Biotechnology and Genetic Engineering, Faculty of Science and Arts, Jordan University of Science and Technology, Irbid, Jordan
2 Department of Applied Biological Sciences, Faculty of Science and Arts, Jordan University of Science and Technology, Irbid, Jordan
3 Department of Public Health, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan
4 Department of Natural Resources and Environment, College of Agriculture, Jordan University of Science and Technology, Irbid, Jordan
5 Department of Biology and Biotechnology, The Hashemite University, Zarqa, Jordan

Caffeine, a purine alkaloid, is reported to act both as an inducer or inhibitor to plant growth in various species. The aim of this study was to examine the effect of exogenous caffeine on tobacco (Nicotiana tabacum) plants, a plant that does not naturally synthesise caffeine. A hydroponic experiment was carried out in a growth chamber for 14 d using Hoagland's solution supplemented with 0 (control), 25, 50, 100, 1,000; and 5,000 μM caffeine. None of the investigated caffeine concentrations significantly decreased the net photosynthetic rate except the highest concentrations of 1,000 and 5,000 μM. Light microscopy of thick-sectioned roots showed that 1,000 μM and 5,000 μM caffeine-treated plants possessed deformed epidermal cells, reduced number of cortical cells, and deformed vascular tissues with cells exhibiting thickened xylem walls as compared with control plants. Moreover, transmission electron micrographs of roots revealed that mitochondria and the plasma membrane were affected.

Keywords: chloroplast; photosynthetic rate; stomatal conductance; transmission microscope; transpiration rate; xylem

Received: May 24, 2017; Accepted: September 5, 2017; Prepublished online: December 1, 2018; Published: November 1, 2018Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Alkhatib, R., Alkhatib, B., Al-Eitan, L., Abdo, N., Tadros, M., & Bsoul, E. (2018). Physio-anatomical responses of tobacco under caffeine stress. Photosynthetica, 56(4), 1140-1146. doi: 10.1007/s11099-018-0798-4.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Alkhatib R., Alkhatib B., AL-Quraan N. et al.: Impact of exogenous caffeine on morphological, biochemical, and ultrastructural characteristics in Nicotiana tabacum var. Turkish.-Biol. Plantarum 60: 706-714, 2016. Go to original source...
    2. Alkhatib R., Bsoul E., Blom D.A. et al.: Microscopic analysis of lead accumulation in tobacco (Nicotiana tabacum var. Turkish) roots and leaves.-J. Microsc. Ultrastruct. 1: 57-62, 2013. Go to original source...
    3. Anaya A., Waller G.R., Okinda-Owuor P. et al.: The Role of Caffeine in the production decline due to autotoxicity in coffee and tea plantations.-In: Reigosa M., Pedrol N. (ed.): Allelopathy: from Molecules to Ecosystems. Pp. 71-91. Science Publishers Inc., Enfield 2002.
    4. Ashihara H., Crozier A.: Biosynthesis and catabolism of caffeine in low-caffeine-containing species of Coffea.-J. Agric. Food Chem. 47: 3425-3431, 1999. Go to original source...
    5. Ashihara H., Crozier A.: Caffeine: a well known but little mentioned compound in plant science.-Trends Plant Sci. 6: 407-413, 2001. Go to original source...
    6. Ashihara H., Sano H., Crozier A.: Caffeine and related purine alkaloids: biosynthesis, catabolism, function and genetic engineering.-Phytochemistry 69: 841-856, 2008. Go to original source...
    7. Ciamporová M.: The development of structural changes in epidermal ceils of maize roots during water stress.-Biol. Plantarum 29: 290-294, 1987. Go to original source...
    8. Ciamporová M., Mistrík I.: The Impact of the environment on roots and root systems the ultrastructural response of root cells to stressful conditions.-Environ. Exp. Bot. 33: 11-26, 1993. Go to original source...
    9. Coetzee J., van der Merwe C.F.: Some characteristics of the buffer vehicle in glutaraldehyde-based fixatives.-J. Microsc. 146: 143-155, 1987. Go to original source...
    10. Curlango-Rivera G., Duclos V.D., Ebolo J.J., Hawes C.M.: Transient exposure of root tips to primary and secondary metabolites: Impact on root growth and production of border cells.-Plant Soil 332: 267-275, 2010. Go to original source...
    11. Dhanapackiam S., Iiyas M.H.: Effect of salinity on chlorophyll and carbohydrate contents of Sesbania grandiflora seedlings.-Indian J. Sci. Technol. 3: 64-66, 2010.
    12. Epstein M.A., Holt S.J.: The localization by electron microscopy of hela cell surface enzymes splitting adenosine triphosphate.-J. Cell. Biol. 19: 325-336, 1963. Go to original source...
    13. Ferreira J.S., Duke S.O., Vaughn K.C.: Histochemical and immunocytochemical localization of tropane alkaloids in Erythroxylum coca var. coca and E. novogranatense var. novogranatense.-Int. J. Plant Sci. 159: 492-503, 1998.
    14. Gilbert G.A., Wilson C., Madore M.A.: Root-zone salinity alters raffinose oligosaccharide metabolism and transport in coleus.-Plant Physiol. 115: 1267-1276, 1997. Go to original source...
    15. Hadacek F., Kraus G.F.: Plant root carbohydrates affect growth behaviour of endophytic microfungi.-FEMS Microbiol. Ecol. 41: 161-170, 2002. Go to original source...
    16. Hajibagheri M.A., Yeo A.R., Flowers T.J.: Salt tolerance in Suaeda maritima (L.) Dum. Fine structure and ion concentrations in the apical regions of roots.-New Phytol. 99: 331-343, 1985. Go to original source...
    17. Hajibagheri M.A., Harvey D.M., Flowers T.J.: Quantitative ion distribution withir/root cells of salt-sensitive and salt-tolerant maize varieties.-New Phytol. 105: 367-379, 1987. Go to original source...
    18. Hollingsworth R.G., Armstrong J.W., Campbell E.: Caffeine as a repellent for slugs and snails.-Nature 417: 915-916, 2002. Go to original source...
    19. Khanam N., Khoo C., Close R., Khan A.G.: Organogenesis, differentiation and histolocalization of alkaloids in cultured tissues and organs of Duboisia myoporoides R. Br.-Ann. Bot.-London 86: 745-752, 2000. Go to original source...
    20. Kim Y.S., Uefuji H., Ogita S., Sano H.: Transgenic tobacco plants producing caffeine: a potential new strategy for insect pest control.-Transgenic Res. 15: 667-672, 2006. Go to original source...
    21. Krapp A., Stitt M.: An evaluation of direct and indirect mechanisms for the "sink-regulation" of photosynthesis in spinach: changes in gas exchange, carbohydrates, metabolites, enzyme activities and steady state transcript levels after coldgirdling source leaves.-Planta 195: 313-323, 1995. Go to original source...
    22. Kumar G., Tripathi A.: Mutagenic response of caffeine in Capsicum annuum.-J. Indian Bot. Soc. 83: 136-140, 2004.
    23. Mathavan S., Premalatha Y., Christopher M.M.: Effects of caffeine and theophylline on the fecundity of four lepidopteran species.-Exp. Biol. 44: 133-138, 1985.
    24. Mohanpuria P., Yadav S.K.: Retardation in seedling growth and induction of early senescence in plants upon caffeine exposure is related to its negative effect on Rubisco.-Photosynthetica 47: 293-297, 2009. Go to original source...
    25. Mondolot L.P., Fisca L.A., Buatois B. et al.: Evolution in caffeoylquinic acid content and histolocalization during Coffea canephora leaf development.-Ann. Bot.-London 98: 33-40, 2006. Go to original source...
    26. Montes O., Diánez F., Camacho F.: Doses of caffeine on the development and performance of pepper crops under greenhouse.-Hortic. Bras. 32: 398-403, 2014. Go to original source...
    27. Nishizawa N.K., Tainaka H., Okubo A. et al.: Desiccationinduced heterophagy in plant root cells.-In: Krebs K.H., Richter H., Hinckley T.M (ed.): Structural and Functional Responses to Environmental Stresses: Water Shortage. Pp. 99-111. SPB Acad. Publ., The Hague 1989.
    28. Pietrini F., Iannelli M.A., Pasqualini S., Massacci A.: Interaction of cadmium with glutathione and photosynthesis in developing leaves and chloroplasts of Phragmites australis (Cav.) Trin. ex Steudel.-Plant Physiol. 133: 829-837, 2003. Go to original source...
    29. Reynolds E.S.: The use of lead citrate at high pH as an electronopaque stain in electron microscopy.-J. Cell Biol. 17: 208-212, 1963. Go to original source...
    30. Spurr A.R.: A low-viscosity epoxy resin embedding medium for electron microscopy.-J. Ultrastruct Res. 26: 31-43, 1969. Go to original source...
    31. Sun A.Z., Guo F.Q.: Chloroplast retrograde regulation of heat stress responses in plants.-Front. Plant Sci. 7: 398, 2016.
    32. Vartapetian B.B., Andreeva I.N., Generozova I.P. et al.: Functional electron microscopy in studies of plant response and adaptation to anaerobic stress.-Ann. Bot.-London 91: 155-172, 2003. Go to original source...
    33. Vinocur B., Altman A.: Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations.-Curr. Opin. Biotechnol. 16: 123-132, 2005. Go to original source...

    Return to the content