Photosynthetica 2018, 56(1):62-66 | DOI: 10.1007/s11099-017-0755-7

Chloroplast ribonucleoprotein-like proteins of the moss Physcomitrella patens are not involved in RNA stability and RNA editing

H. Uchiyama1, M. Ichinose1,2, M. Sugita1,*
1 Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya, Japan
2 Institute of Transformative Bio-Molecules, Nagoya University, Chikusa-ku, Nagoya, Japan

Many RNA recognition motif (RRM)-containing proteins are known to exist in chloroplasts. Major members of the RRM protein family, which are chloroplast ribonucleoproteins (cpRNPs), have been investigated in seed plants, including tobacco and Arabidopsis thaliana, but never in early land plants, such as bryophytes. In this study, we surveyed RRM proteins encoded in the moss Physcomitrella patens genome and predicted 25 putative chloroplast RRM proteins. Among them, two RRM-containing proteins, PpRBP2a and PpRBP2b, resembled cpRNPs and were thus referred to as cpRNP-like proteins. However, knockout mutants of either one or two PpRBP2 genes exhibited a wild type-like phenotype. Unlike Arabidopsis cpRNPs, the levels of mRNA accumulation in chloroplasts were not affected in the PpRBP2 knockout mutants. In addition, the efficiency of RNA editing was also not altered in the mutants. This suggests that PpRBP2a and 2b may be functionally distinct from Arabidopsis cpRNPs.

Keywords: pentatricopeptide repeat protein; photosynthesis gene; posttranscriptional regulation; RNA-binding protein; RNA processing

Received: April 29, 2017; Accepted: July 17, 2017; Published: March 1, 2018Show citation

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Uchiyama, H., Ichinose, M., & Sugita, M. (2018). Chloroplast ribonucleoprotein-like proteins of the moss Physcomitrella patens are not involved in RNA stability and RNA editing. Photosynthetica, 56(1), 62-66. doi: 10.1007/s11099-017-0755-7.
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