Photosynthetica 2014, 52(2):193-202 | DOI: 10.1007/s11099-014-0020-2

Different drought-stress responses in photosynthesis and reactive oxygen metabolism between autotetraploid and diploid rice

P. M. Yang1,2, Q. C. Huang1, G. Y. Qin1,*, S. P. Zhao1, J. G. Zhou2
1 Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou, Henan, China
2 Henan Institute of Science and Technology, Xinxiang, Henan, China

Photosynthetic light curve, chlorophyll (Chl) content, Chl fluorescence parameters, malondialdehyde (MDA) content, phosphoenolpyruvate carboxylase (PEPC) activity and reactive oxygen metabolism were studied under drought stress in two autotetraploid rice lines and corresponding diploid rice lines. Net photosynthetic rate decreased dramatically, especially under severe drought stress and under high photosynthetic active radiation in diploid rice, while it declined less under the same conditions in autotetraploid lines. Compared with the corresponding diploid lines, the Chl content, maximum photochemical efficiency of photosystem (PS) II, and actual photochemical efficiency of PSII were reduced less in autotetraploid lines. PEPC activities were higher in autotetraploid rice lines. PEPC could alleviate inhibition of photosynthesis caused by drought stress. The chromosome-doubling enhanced rice photoinhibition tolerance under drought stress. The lower MDA content and superoxide anion production rate was found in the autotetraploid rice indicating low peroxidation level of cell membranes. At the same time, the superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities were higher in autotetraploid rice lines. SOD, POD, and CAT could effectively diminish the reactive oxygen species and reduced the membrane lipid peroxidation.

Keywords: chlorophyll fluorescence; Oryza sativa; photosynthetic light curve; reactive oxygen species

Received: March 20, 2013; Accepted: July 1, 2013; Published: June 1, 2014Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Yang, P.M., Huang, Q.C., Qin, G.Y., Zhao, S.P., & Zhou, J.G. (2014). Different drought-stress responses in photosynthesis and reactive oxygen metabolism between autotetraploid and diploid rice. Photosynthetica, 52(2), 193-202. doi: 10.1007/s11099-014-0020-2.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Bandyopadhyay, A., Datta, K., Zhang, J. et al.: Enhanced photosynthesis rate in genetically engineered indica rice expressing pepc gene cloned from maize. - Plant Sci. 172: 1204-1209, 2007. Go to original source...
    2. Blakeslee, A.F., Avery, A.G.: Methods of inducing doubling of chromosomes in plants by treatment with colchicine. - J. Hered. 28: 393-411, 1937. Go to original source...
    3. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    4. Cai, D.-T., Chen, J.-G., Chen, D.-L. et al.: Exploring new way to solve the potential crisis of food shortage by polyploid rice. - In: 5th International Rice Genetics Symposium. 19-23 Nov. Manila, Philippines. IRRI, Pp. 58, Manila 2005.
    5. Cakmak, I., Marschner, H.: Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. - Plant Physiol. 98: 1222-1227, 1992. Go to original source...
    6. Chaves, M.M.: Effects of water deficits on carbon assimilation. - J. Exp. Bot. 42: 1-16, 1991. Go to original source...
    7. Chen, Z.-Y., Wu, D.-Y, Song, W.-C., Zhang, Y.-H., Qin, R.-Z., Bao, W.-K.: Recent advances in the autotetraploid rice breeding. - Sci. Agric. Sin. 20: 20-24, 1987.
    8. Cornic, G., Briantais, J. M.: Partitioning of photosynthetic electron flow between CO2 and O2 reduction in a C3 leaf (Phaseolus vulgaris L.) at different CO2 concentrations and during drought stress. - Planta 183: 178-184, 1991. Go to original source...
    9. Cornic, G.: Drought stress and high light effects on leaf photosynthesis. - In: Baker N.R., Bowyer J.R. (ed.): Photoinhibition of Photosynthesis: from Molecular Mechanisms to the Field. Pp. 297-313. BIOS Scientific Publ., Oxford 1994.
    10. Dias, M.C., Brüggemann, W.: Differential inhibition of photosynthesis under drought stress in Flaveria species with different degrees of development of the C4 syndrome. - Photosynthetica 45: 75-84, 2007. Go to original source...
    11. Ding, Z.-S., Zhou, B.-Y., Sun, X.-F., Zhao, M.: [High light tolerance is enhanced by overexpressed PEPC in rice under drought stress.] - Acta Agron. Sin. 38: 285-292, 2012. [In Chinese] Go to original source...
    12. Doubnerová, V., Ryšlavá, H.: What can enzymes of C4 photosynthesis do for C3 plants under stress. - Plant Sci. 180: 575-583, 2011. Go to original source...
    13. Duff, S.M.G and Chollet, R.: In vivo regulation of wheat-leaf phosphoenolpyruvate carboxylase by reversible phosphorylation. - Plant Physiol. 107: 775-782, 1995. Go to original source...
    14. Fang, L.-F., Ding, Z.-S., Zhao, M.: [Characteristics of drought tolerance in ppc overexpressed rice seedlings.] - Acta Agron. Sin. 34: 1220-1226, 2008. [In Chinese] Go to original source...
    15. Foyer, C.H., Noctor, G.: Oxygen processing in photosynthesis: regulation and signalling. - New Phytol. 146: 359-388, 2000. Go to original source...
    16. Foyer, C.H., Noctor, G.: Redox sensing and signalling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. - Physiol. Plantarum 119: 355-364, 2003. Go to original source...
    17. Genty, B., Briantais, J.M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. Biophys. Acta: 990: 87-92, 1989. Go to original source...
    18. Gonzalez, M.C., Sanchez, R., Cejudo, F.J.: Abiotic stresses affecting water balance induce phosphoenolpyruvate carboxylase expression in roots of wheat seedlings. - Planta 216: 985-992, 2003.
    19. Gueta-Dahan, Y., Yaniv, Z., Zilinskas, B.A., Ben-Hayyim, G.: Salt and oxidative stress: similar and specific response and their relation to salt tolerance in citrus. - Planta 203: 460-469, 1997. Go to original source...
    20. Hipkins, M.F., Baker, N.R.: Spectroscopy. - In: Hipkins, M.F Baker NR (ed.): Photosynthesis Energy Transduction: A Practical Approach. Pp 63-64. IRL Press, Oxford 1986.
    21. Jeanneau, M., Gerentes, D., Foueillassar, X. et al.: Improvement of drought tolerance in maize: towards the functional validation of the Zm-Asr1 gene and increase of water use efficiency by over-expressing C4-PEPC. - Biochimie 84: 1127-1135, 2002. Go to original source...
    22. Jiao, D.-M., Huang, X.-Q., Li, X. et al.: Photosynthetic characteristics and tolerance to photo-oxidation of transgenic rice expressing C4 photosynthesis enzymes. - Photosynth Res. 72: 85-93, 2002. Go to original source...
    23. Jiao, D.-M., Li, X., Ji, B.-H.: Photoprotective effects of high level expression of C4 phosphoenolpyruvate carboxylase in transgenic rice during photoinhibition. - Photosynthetica 43: 501-508, 2005. Go to original source...
    24. Kutschera, U., Kohler, K.: Turgor pressure and elongation growth in developing sunflower hypocotyls. - J. Plant Physiol. 69: 1145-1149, 1993. Go to original source...
    25. Lawlor, D.W., Cornic, G.: Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. - Plant Cell Environ. 25: 275-294, 2002. Go to original source...
    26. Lebouteiller, B., Gousset-Dupont, A., Pierre, J.N. et al.: Physiological impacts of modulating phosphoenolpyruvate carboxylase levels in leaves and seeds of Arabidopsis thaliana. - Plant Sci. 17: 265-272, 2007. Go to original source...
    27. Maroco, J.P., Rodrigues, M.L., Lopes, C., Chaves, M.M.: Limitations to leaf photosynthesis in field-grown grapevine under drought-metabolic and modelling approaches. - Funct. Plant Biol. 29: 451-459, 2002. Go to original source...
    28. Naumann, J.C., Young, D.R., Anderson, J.E.: Linking leaf chlorophyll fluorescence properties to physiological responses for detection of salt and drought stress in coastal plant species. - Physiol. Plantarum 131: 422-433, 2007. Go to original source...
    29. Oukarroum, A., Schansker, G., Strasser, R.J.: Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering. - Environ. Exp. Bot. 60: 438-446, 2007. Go to original source...
    30. Oukarroum, A., Schansker, G., Strasser, R.J.: Drought stress effects on photosystem I content and photosystem II thermotolerance analyzed using Chl a fluorescence kinetics in barley varieties differing in their drought tolerance. - Physiol. Plantarum 137: 188-199, 2009. Go to original source...
    31. Pantuwan, G., Fukai, S., Cooper, M., Rajatasereekul, S., and O'Toole, J.C.: Yield response of rice (Oryza sativa L.) genotypes to drought under rainfed lowlands 3. Plant factors contributing to drought resistance. - Field Crop Res. 73: 181-200, 2002. Go to original source...
    32. Powles, S.B.: Photoinhibition of photosynthesis induced by visible light. - Annu. Rev. Plant Physiol. 35: 892-898, 1984. Go to original source...
    33. Puliga, S., Vazzana, C., Davies, W.J.: Control of crop leaf growth by chemical and hydraulic influences. - J. Exp. Bot. 47: 529-537, 1996. Go to original source...
    34. Pütter, J.: Peroxidases. - In: Bergmeyer, H.U. (ed.): Methods of Enzymatic Analysis. Vol. 2. Pp. 685-690. Academic Press, New York 1974. Go to original source...
    35. Sanchez, R., Cejudo, F.J.: Identification and expression analysis of a gene encoding a bacterial-type phosphoenolpyruvate carboxylase from Arabidopsis and rice. - Plant Physiol. 132: 949-957, 2003. Go to original source...
    36. Sanchez, R., Flores, A., Cejudo, F.J.: Arabidopsis phosphoenolpyruvate carboxylase genes encode immunologically unrelated polypeptides and are differentially expressed in response to drought and salt stress. - Planta 223: 901-909, 2006. Go to original source...
    37. Scandalios, J.G.: Oxygen stress and superoxide dismutases. - Plant Physiol. 101: 7-12, 1993. Go to original source...
    38. Song, W.-C., Zhang, Y.-H.: Rice tetraploidy and its effect on agronomic traits and nutritional constituents. - Acta Agron. Sin. 18: 137-144, 1992.
    39. Tu, S.-B., Luan, L., Liu, Y.-H. et al.: Production and heterosis analysis of rice autotetraploid hybrids. - Crop Sci. 47: 2356-2363, 2007. Go to original source...
    40. Valentini, R., Epron, D., De Angelis, P. et al.: In situ estimation of net CO2 assimilation, photosynthetic electron flow and photo-respiration in Turkey oak (Q. cerris L.) leaves: diurnal cycles under different levels of water supply. - Plant Cell Environ. 18: 631-640, 1995. Go to original source...
    41. Valladares, F., Pearcy, R.W.: Drought can be more critical in the shade than in the sun: a field study of carbon gain and photoinhibition in a Californian shrub during a dry El Niño year. - Plant Cell Environ. 25: 749-759, 2002. Go to original source...
    42. van Heerden, P.D.R., Swanepoel, J.W., Krüger, G.H.J.: Modulation of photosynthesis by drought in two desert scrub species exhibiting C3-mode CO2 assimilation. - Environ. Exp. Bot. 61: 124-136, 2007. Go to original source...
    43. Wang, A.-G., Luo, G.-H.: [Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants.] - Plant Physiol. Commun. 26: 55-57, 1990. [In Chinese]
    44. Yordanov, I., Velikova, V., Tsonev, T.: Plant responses to drought, acclimation, and stress tolerance. - Photosynthetica 38: 171-186, 2000. Go to original source...
    45. Yordanov, I., Velikova, V., Tsonev, T.: Plant responses to drought and stress tolerance. - Bulg. J. Plant Physiol, Special Issue 2003. Pp. 187-206, 2003.
    46. Yoshida, M., Nouchi, I., Toyama, S.: Studies on the role of active oxygen in ozone injury to plant cells. I. Generation of active oxygen in rice protoplasts exposed to ozone. - Plant Sci. 95: 197-205, 1994. Go to original source...
    47. Zhou, B.-Y., Ding, Z.-S., Zhao, M.: [Alleviation of drought stress inhibition on photosynthesis by over expression of PEPC gene in rice.] - Acta Agron. Sin. 37: 112-118, 2011. [In Chinese] Go to original source...

    Return to the content