Photosynthetica, 2008 (vol. 46), issue 1

Photosynthetica 2008, 46(1):35-39 | DOI: 10.1007/s11099-008-0007-y

Photosystem 2 photochemistry and pigment composition of a yellow mutant of rice (Oryza sativa L.) under different irradiances

Q. Chen1, L. F. Wang1, N. Su1, H. D. Qin2, H. B. Niu2, J. L. Wang1, H. Q. Zhai1, J. M. Wan1,2,*
1 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
2 National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, China

A yellow leaf colouration mutant (named ycm) generated from rice T-DNA insertion lines was identified with less grana lamellae and low thylakoid membrane protein contents. At weak irradiance [50 µmol(photon) m-2 s-1], chlorophyll (Chl) contents of ycm were ≈20 % of those of WT and Chl a/b ratios were 3-fold that of wild type (WT). The leaf of ycm showed lower values in the actual photosystem 2 (PS2) efficiency (ΦPS2), photochemical quenching (qP), and the efficiency of excitation capture by open PS2 centres 1 (Fv'/Fm') than those of WT, except no difference in the maximal efficiency of PS2 photochemistry (Fv/Fm). With progress in irradiance [100 and 200 µmol(photon) m-2 s-1], there was a change in the photosynthetic pigment stoichiometry. In ycm, the increase of total Chl contents and the decrease in Chl a/b ratio were observed. ΦPS2, qP, and Fv'/Fm' of ycm increased gradually along with the increase of irradiance but still much less than in WT. The increase of xanthophyll ratio [(Z+A)/(V+A+Z)] associated with non-photochemical quenching (qN) was found in ycm which suggested that ycm dissipated excess energy through the turnover of xanthophylls. No significant differences in pigment composition were observed in WT under various irradiances, except Chl a/b ratio that gradually decreased. Hence the ycm mutant developed much more tardily than WT, which was caused by low photon energy utilization independent of irradiance.

Keywords: β-carotene; chlorophyll; photochemical and non-photochemical quenching; xanthophyll cycle

Received: March 9, 2007; Accepted: May 2, 2007; Published: March 1, 2008Show citation

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Chen, Q., Wang, L.F., Su, N., Qin, H.D., Niu, H.B., Wang, J.L., Zhai, H.Q., & Wan, J.M. (2008). Photosystem 2 photochemistry and pigment composition of a yellow mutant of rice (Oryza sativa L.) under different irradiances. Photosynthetica, 46(1), 35-39. doi: 10.1007/s11099-008-0007-y.
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    References

    1. Campbell, D., Hurry, V., Clarke, A.K., Gustafsson, P., Öquist, G.: Chlorophyll fluorescence analysis of cyanobacterial photosynthesis and acclimation.-Microbiol. mol. Biol. Rev. 62: 667-683, 1998.
    2. Demmig-Adams, B., Adams, W.W., III: Photoprotection and other responses of plants to light stress.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 599-626, 1992. Go to original source...
    3. Demmig-Adams, B., Adams, W.W., III: The role of xanthophyll cycle carotenoids in the protection of photosynthesis.-Trends Plant Sci. 1: 21-26, 1996. Go to original source...
    4. Demmig-Adams, B., Adams, W.W., III, Barker, D.H., Logan, B.A., Bowling, D.R., Verhoeven, A.S.: Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation.-Physiol. Plant. 98: 253-264, 1996.
    5. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    6. Hsu, B.D., Lee, J.Y.: The photosystem II heterogeneity of chlorophyll b-deficient mutants of rice: a fluorescence induction study.-Plant Physiol. 22: 195-200, 1995. Go to original source...
    7. Jung, K.H., Hur, J.H., Ryu, C.H., Choi, Y.J., Chung, Y.Y., Miyao, A., Hirochika, H., An, G.H.: Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system.-Plant Cell Physiol. 44: 463-472, 2003. Go to original source...
    8. Krause, G.H., Cornic, G.: CO2 and O2 interactions in photoinhibition.-In: Kyle, D.J., Osmond, C.B., Arntzen, C.J. (ed.): Photoinhibition. Pp. 169-196. Elsevier, Amsterdam-New York-Oxford 1987.
    9. Kusumi, K., Komori, H., Satoh, H., Iba, K.: Characterization of a zebra mutant of rice with increased susceptibility to light stress.-Plant Cell Physiol. 41: 158-64, 2000. Go to original source...
    10. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    11. Lee, S., Kim, J.H., Yoo, E.S., Lee, C.H., Hirohiko, H., An, G.H.: Differential regulation of chlorophyll a oxygenase genes in rice.-Plant mol. Biol. 57: 805-818, 2005. Go to original source...
    12. Lichtenthaler, H.K.: Chlorophylls and carotenoids-pigments of photosynthetic membranes.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350-382. Academic Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1987. Go to original source...
    13. Lu, C.M., Lu, Q.T., Zhang, J.H., Kuang, T.Y.: Characterization of photosynthetic pigment composition, Photosystem II photochemistry and thermal energy dissipation during leaf senescence of wheat plants grown in the field.-J. exp. Bot. 52: 1805-1810, 2001.
    14. Lu, C.M., Zhang, J.H.: Heat-induced multiple effects on PSII in wheat plants.-J. Plant Physiol. 156: 259-265, 2000a. Go to original source...
    15. Lu, C.M., Zhang, J.H.: Photosystem II photochemistry and its sensitivity to heat stress in maize plants as affected by nitrogen deficiency.-J. Plant Physiol. 157: 124-130, 2000b. Go to original source...
    16. Masuda, T., Fusada, N., Oosawa, N., Takamatsu, K., Yamamoto, Y.Y., Ohto, M., Nakamura, K., Goto, K., Shibata, D., Shirano, Y., Hayashi, H., Kato, T., Tabata, S., Shimada, H., Ohta, H., Takamiya, K.: Functional analysis of isoforms of NADPH:protochlorophyllide oxidoreductase (POR), PORB and PORC, in Arabidopsis thaliana.-Plant Cell Physiol. 44: 963-974, 2003. Go to original source...
    17. Öquist, G., Huner, N.P.A.: Cold-hardening-induced resistance to photoinhibition of photosynthesis in winter rye is dependent upon an increased capacity for photosynthesis.-Planta 189: 150-156, 1993. Go to original source...
    18. Peng, L.W., Ma, J.F., Chi, W., Guo, J.K., Zhu, S.Y., Lu, Q.T., Lu, C.M., Zhang, L.X.: Low PS2 ACCUMULATION1 is involved in efficient assembly of photosystem II in Arabidopsis thaliana.-Plant Cell 18: 955-969, 2006. Go to original source...
    19. Sugimoto, H., Kusumi, K., Tozawa, Y., Yazaki, J., Kishimoto, N., Kikuchi, S., Iba, K.: The virescent-2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation.-Plant Cell Physiol. 45: 985-996, 2004. Go to original source...
    20. Terao, T., Yamashita, A., Katoh, S.: Chlorophyll b-deficient mutants of rice. I. Absorption and fluorescence spectra and chlorophyll a/b ratios.-Plant Cell Physiol. 26: 1361-1367, 1985.
    21. Thayer, S.S., Björkman, O.: Leaf xanthophyll content and composition in sun and shade determined by HPLC.-Photosynth. Res. 23: 331-343, 1990. Go to original source...
    22. Wang, L.F., Ji, H.B., Bai, K.Z., Li, L.B., Kuang, T.Y.: Photosystem 2 activities of hyper-accumulator Dicranopteris dichotoma Bernh from a light rare earth elements mine.-Photosynthetica 44: 202-207, 2006. Go to original source...
    23. Zhang, H.T., Li, J.J., Yoo, J.H., Yoo, S.C., Cho, S.H., Koh, H.J., Seo, H.S., Paek, N.C.: Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development.-Plant mol. Biol. 62: 325-337, 2006. Go to original source...

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