Photosynthetica 2012, 50(1):120-128 | DOI: 10.1007/s11099-012-0021-y

Transgenic tomato plants overexpressing chloroplastic monodehydroascorbate reductase are resistant to salt- and PEG-induced osmotic stress

F. Li1, Q. Y. Wu3, M. Duan2, X. C. Dong2, B. Li2, Q. W. Meng2,*
1 Department of Neurobiology, Xuzhou Medical College, Xuzhou, Jiangsu, P.R. China
2 College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, P.R. China
3 The Key Laboratory of Transplantation Immunity, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, P.R. China

RNA gel hybridization showed that the expression of monodehydroascorbate reductase (MDHAR) in the wild type (WT) tomato was decreased firstly and then increased under salt- and polyethylene glycol (PEG)-induced osmotic stress, and the maximum level was observed after treatment for 12 h. WT, sense transgenic and antisense transgenic tomato plants were used to analyze the antioxidative ability to cope with osmotic stresses. After salt stress, the fresh mass (FM) and height of sense transgenic lines were greater than those of antisense lines and WT plants. Under salt and PEG treatments, sense transgenic plants showed a lower level of hydrogen peroxide (H2O2) and malondialdehyde (MDA), a higher net photosynthetic rate (P N), and the maximal photochemical efficiency of PSII (Fv/Fm) compared with WT and antisense transgenic plants. Moreover, sense lines maintained higher ascorbate peroxidase (APX) activity than WT and antisense plants under salt- and PEG-induced osmotic stress. These results indicate that chloroplastic MDHAR plays an important role in alleviating photoinhibition of PSII by elevating ascorbate (AsA) level under salt- and PEG-induced osmotic stress.

Keywords: ascorbate; monodehydroascorbate reductase; osmotic stresses; reactive oxygen species; tomato

Received: March 22, 2011; Accepted: December 28, 2011; Published: March 1, 2012Show citation

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Li, F., Wu, Q.Y., Duan, M., Dong, X.C., Li, B., & Meng, Q.W. (2012). Transgenic tomato plants overexpressing chloroplastic monodehydroascorbate reductase are resistant to salt- and PEG-induced osmotic stress. Photosynthetica, 50(1), 120-128. doi: 10.1007/s11099-012-0021-y.
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