Photosynthetica 2015, 53(4):499-505 | DOI: 10.1007/s11099-015-0123-4

Photosynthesis of a yellow-green mutant line in maize

X. M. Zhong1, S. F. Sun1, F. H. Li1, J. Wang1, Z. S. Shi1,*
1 Special Corn Institute of Shenyang Agricultural University, Shenyang, China

This study compared the relationship between chlorophyll (Chl) content, gas exchange, Chl fluorescence characteristics, and leaf color, using paired near-isogenic lines (NILs) of a medium-green leaf inbred line SN12 and a yellow-green leaf mutant SN62 to explore the photosynthesis of the yellow-green mutant. The SN62 was found in a female parent, Xianyu 335, which grew normally, although there were small yellow spots on the leaves at the seedling stage and yellow-green leaves appeared from the seedling to the maturation stage. The results indicated that Chl a (b), quantum efficiency of PSII, and maximal quantum yield of PSII photochemistry of SN62 were significantly lower than those of SN12, but there were almost no differences in the net photosynthetic rate (P N). There was no significant correlation between Chl a (b) and P N of inbred lines with different leaf colors. In the reproductive stage, photochemical quenching, effective quantum yield of PSII photochemistry, and the electron transport rate of SN62 increased obviously, and all parameter values exceeded the values of SN12. It explained that increasing the openness of the PSII reaction center was able to compensate for the lower Chl content, which was beneficial for harvesting more light energy for photochemical reactions. It also ensured that P N was not reduced.

Keywords: gene mutation; photochemical reaction; potential productivity; photosynthesis

Received: May 22, 2014; Accepted: November 20, 2014; Published: December 1, 2015Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Zhong, X.M., Sun, S.F., Li, F.H., Wang, J., & Shi, Z.S. (2015). Photosynthesis of a yellow-green mutant line in maize. Photosynthetica, 53(4), 499-505. doi: 10.1007/s11099-015-0123-4.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Buckner B., Robertson D.S.: Cytogenetic localization of the y1, l10, l12, ms1 and si1 loci. - Maize Genet. Coop Newsl. 67: 64-65, 1993.
    2. Carol P., Stevenson D., Bisanz C. et al.: Mutations in the Arabidopsis gene IMMUTANTS cause a variegated phenotype by inactivating a chloroplast terminal oxidase associated with phytoene desaturation. - Plant Cell 11: 57-68, 1999. Go to original source...
    3. Chen J.F.: Inheritance of a leaf mutant in maize. Master Thesis, Henan Agri. Univ. 2008
    4. Dai X.B., Cao S.Q., Xu X.M. et al.: [Study on a mutant with low content chlorophyll b in a high yielding rice and its photosynthesis properties.] - Acta Bot. Sin. 42: 1289-1294, 2000. [In Chinese]
    5. Dong F.G., Zhu X.D., Xiong Z.M. et al.: [Breeding of a photothermoperiod sensitive genie male sterile indica rice with a pale-green-leaf marker.] - Chin. J. Rice Sci. 9: 65-70, 1995. [In Chinese]
    6. Falbel T.G., Meehl J.B., Staehelin L.A.: Severity of mutant phenotype in a series of chlorophyll-deficient wheat mutants depends on light intensity and the severity of the block in chlorophyll synthesis. - Plant Physiol. 112: 821-832, 1996. Go to original source...
    7. Falbel T.G., Staehelin L.A.: Partial block in the early steps of the chlorophyll synthesis pathway: A common feature of chlorophyll-deficient mutants. - Plant Physiol. 97: 311-320, 1996. Go to original source...
    8. Genty B., Briantais J.M., Baker N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. Biophys. Acta 990: 87-92, 1989. Go to original source...
    9. Ghirardi M.L., Melis A.: Chlorophyll b deficiency in soybean mutants effects on photosystem stoichiometry and chlorophyll antenna size. - BBA-Bioenergetics 932: 130-137, 1988. Go to original source...
    10. Greene B.A., Allred D.R., Morishige D.T., Staehelin L.A.: Hierarchical response of light harvesting chlorophyll proteins in a light-sensitive chlorophyll b-deficient mutant of maize. - Plant Physiol. 87: 357-364, 1998.
    11. Hoisington D., Neuffer M.G..: Nec4, a new necrotic mutant on 2S. - Maize Genet. Coop Newsl. 57: 159-160, 1983.
    12. Hu Z., Peng L.P., Cai Y.H.: [A yellow-green nucleus mutant of rice.] - Acta Genet Sin. 8: 256-261, 1981. [In Chinese]
    13. Krause G..H., Weis E.: Chlorophyll fluorescence and photosynthesis: The Basics. - Annu. Rev. Plant. Phys. 42: 313-349, 1991. Go to original source...
    14. Król M., Spangfort M.D., Huner N.P.: Chlorophyll a/b binding proteins, pigment conversions and early light induced proteins in a chlorophyll bless barley mutant. - Plant Physiol. 107: 873-883, 1995. Go to original source...
    15. Kurata N., Miyoshi K., Nonomura K. I. et al.: Rice mutants and genes related to organ development, morphogenesis and physiological traits. - Plant Cell Physiol. 46: 48-62, 2005. Go to original source...
    16. Larkin R.M., Alonso J.M., Ecker J.R. et al.: GUN4, a regulator of chlorophyll synthesis and intracellular signaling. - Science 299: 902-906, 2003. Go to original source...
    17. Lichtenthaler H,K, Buschmann C.: Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. - In: Wrolstad R.E. (ed.): Current Protocols in Food Analytical Chemistry. Pp. F4.3.1-F4.3.8. John Wiley and Sons, New York 2001. Go to original source...
    18. Lin Y.Q., Liu S., Fu Y.P. et al. [Chlorophyll contents and net photosynthetics rates of T-DNA inserted rice mutant population.] - Chin. J. Rice Sci. 17: 369-372, 2003. [In Chinese]
    19. Lin Z.H., Chen L.S., Chen R.B. et al.: [CO2 assimilation, ribulose-1,5-bisphosphate carboxylase/oxygenase, carbohydrates and photosynthetic electron transport probed by the JIP-test, of tealeaves in response to phosphorus supply.] - BMC Plant Biol. 9: 43, 2009. [In Chinese] Go to original source...
    20. Pan R.Z., Dong Y.D.: [Plant Physiology.] Pp. 77-79. Higher Education Press, Beijing 1995. [In Chinese]
    21. Ramesh K., Chandrasekaran B., Balasubramanian T.N. et al.: Chlorophyll dynamics in rice (Oryza sativa) before and after flowering based on SPAD (chlorophyll) meter monitoring and its relation with grain yield. - J. Agron. Crop. Sci. 188: 102-105, 2002. Go to original source...
    22. Sawers R.J., Viney J., Farmer P.R. et al.: The maize oil yellow1 (oy1) gene encodes the I subunit of magnesium chelatase. - Plant Mol. Biol. 60: 95-106, 2006. Go to original source...
    23. Suzuki J.Y., Bollivar D.W., Bauer C.E.: Genetic analysis of chlorophyll biosynthesis. - Annu Rev. Genet. 31: 61-69, 1997. Go to original source...
    24. van Kooten O., Snel J.F.H.: The use of chlorophyll fluorescence nomenclature in plant stress physiology. - Photosynth. Res. 25: 147-150, 1990. Go to original source...
    25. Xu X.M., Zhang R.X., Tang Y.L.: [Effect of low content chlorophyll on distribution properties of absorbed light energy in leaves of mutant rice.] - Sci. Agric. Sin. 37: 339-343, 2004. [In Chinese]
    26. Zhang Z.S., Yang C., Gao H.Y. et al.: [Relationship between photochemistry activity and decrease in chlorophyll content during senescence in leaves of stay green and quick-leafsenescence inbred line of maize.] - Sci. Agric. Sin. 45: 4794-4800, 2012. [In Chinese]
    27. Zhao H.J., Wu D.X., Shu Q.Y. et al.: [Breeding and characteristics of photo-thermo sensitive genic male sterile rice yutu s labeled with green-revertible albino leaf marker.] - Chin. J. Rice Sci. 18: 515-521, 2004. [In Chinese]
    28. Zhao Y., Du L.F., Yang S.H. et al.: [Chloroplast composition and structure differences in a chlorophyll-reduced mutant of oilseed rape seedlings.] - Acta Bot. Sin. 43: 877-880, 2001. [In Chinese]
    29. Zhao Y., Wang M.L., Zhang Y.Z. et al.: [A chlorophyll-reduced seedling mutant in oilseed rape, Brassica napus, for utilization in F1 hybrid production.] - Plant Breeding 119: 131-135, 2000. [In Chinese] Go to original source...

    Return to the content