Photosynthetica 2018, 56(1):236-243 | DOI: 10.1007/s11099-017-0750-z

Influence of the disaccharide trehalose on the oxidizing side of photosystem II

M. D. Mamedov1,*, E. S. Nosikova2, L. A. Vitukhnovskaya1, A. A. Zaspa1, A. Yu. Semenov1
1 Belozersky Institute of Physical-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia
2 Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia

The steady-state oxygen evolution rate was previously shown to be stimulated by the disaccharide trehalose in PSII suspension. Here we showed a similar increase in the rate of oxygen evolution in PSII core complexes from spinach in solution and in proteoliposomes in the presence of trehalose. Using direct electrometrical technique, we also revealed that trehalose had no effect on the kinetics of electron transfer from Mn to redox-active-tyrosyl radical, YZ* (S1 → S2 transition), while it accelerated the kinetics of electrogenic proton transport during S2 → S3 and S4 → S0 transitions of the wateroxidizing complex (WOC) induced by the first, second, and third laser flashes in dark-adapted PSII samples. These observations imply that the effect of trehalose occurrs due to its interaction with the WOC.

Keywords: effective functioning; osmolyte; photoelectric response; vectorial transfer

Received: April 26, 2017; Accepted: June 26, 2017; Published: March 1, 2018Show citation

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Mamedov, M.D., Nosikova, E.S., Vitukhnovskaya, L.A., Zaspa, A.A., & Yu. Semenov, A. (2018). Influence of the disaccharide trehalose on the oxidizing side of photosystem II. Photosynthetica, 56(1), 236-243. doi: 10.1007/s11099-017-0750-z.
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