Photosynthetica 2014, 52(4):614-626 | DOI: 10.1007/s11099-014-0069-y

A portable reflectance-absorptance-transmittance meter for photosynthetic work on vascular plant leaves

R. J. Ritchie1,*, J. W. Runcie2,3
1 Tropical Environmental Plant Biology Unit, Faculty of Technology and Environment, Prince of Songkla University, Phuket, Thailand
2 School of Biological Sciences, University of Sydney, Sydney, Australia
3 Aquation Pty Ltd, Umina Beach, Australia

PAM (pulse amplitude modulation) fluorometers can be used to estimate the electron transport rate (ETR) [μmol(e-) m-2 s-1] from photosynthetic yield determinations, provided the absorptance (Abtλ) of the photoorganism is known. The standard assumed value used for absorptance is 0.84 (leaf absorptance factor, AbtF). We described a reflectance-absorptancetransmittance (RAT) meter for routine experimental measurements of the actual absorptance of leaves. The RAT uses a red-green-blue (RGB) LED diode light source to measure absorptances at wavelengths suitable for use with PAM fluorometers and infrared gas analysers. Results using the RAT were compared to Abtλ spectra using a Taylor integrating sphere on bird's nest fern (Asplenium nidus), banana, Doryanthes excelsa, Kalanchoe daigremontiana, and sugarcane. Parallel venation had no significant effect upon Abt465 in banana, Doryanthes, a Dendrobium orchid, pineapple, and sugarcane, but there was a slight difference in the case of the fern A. nidus. The average Abt465 (≈ 0.96) and Abt625 (≈ 0.89) were ≈14% and 6% higher than the standard value (AbtF = 0.84). The PAR-range Abt400-700 was only ≈ 5% higher than the standard value (≈ 0.88) based on averaged absorptance from the blue, green, and red light data and from where the RGB-diode was used as a 'white' light source. In some species, absorptances at blue and red wavelengths are quite different (e.g. water lily). Reflectance measurements of leaves using the RAT would also be useful for remote sensing studies.

Keywords: absorptance; electron transport rate; integrating sphere; leaf absorptance factor; PAM fluorometry; reflectance

Received: September 10, 2013; Accepted: May 2, 2014; Published: December 1, 2014Show citation

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Ritchie, R.J., & Runcie, J.W. (2014). A portable reflectance-absorptance-transmittance meter for photosynthetic work on vascular plant leaves. Photosynthetica, 52(4), 614-626. doi: 10.1007/s11099-014-0069-y.
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