Photosynthetica 2000, 38(2):215-220 | DOI: 10.1023/A:1007265715108

Activities of Phosphoenolpyruvate Carboxylase and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in Leaves and Fruit Pericarp Tissue of Different Coffee (Coffea sp.) Genotypes

Y. Lopez1, N. Riaño2, P. Mosquera3, A. Cadavid3, J. Arcila3
1 Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia, Palmira -, Colombia
2 Fisiología Vegetal, Centro Nacional de Investigaciones de Café, CENICAFE, Chinchiná, Colombia
3 Fisiología Vegetal, Centro Nacional de Investigaciones de Café, CENICAFE, Chinchiná, Colombia

In order to study photosynthetic characteristics, phosphoenolpyruvate carboxylase (PEPC) and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activities as well as soluble protein and chlorophyll contents were determined in leaf and fruit pericarp samples from diverse coffee genotypes (Coffea arabica cv. Colombia, Caturra, Caturra Erecta, San Pacho, Tipica, C. stenophylla, C. eugenioides, C. congensis, C. canephora, C. canephora cv. Arabusta, C. arabica cv. Caturra×C. canephora and Hibrido de Timor. We found a slightly higher PEPC activity in fruit pericarp than in leaves, while RuBPCO activity was much lower in pericarp than leaf tissue. Partial purification of PEPC and RuBPCO was carried out from leaves of C. arabica cv. Caturra and Michaelis-Menten kinetics for RuBPCO (Km CO2 = 5.34 µM), (Km RuBP = 9.09 µM) and PEPC (Km PEP = 19.5 µM) were determined. Leaf tissues of Colombia, Hibrido de Timor, and Caturra consistently showed higher content of protein [55.4-64.4 g kg-1 (f.m.)] than San Pacho, C. stenophylla, Tipica, Caturra Erecta, and Caturra×C. canephora [25.6-36.9 g kg-1 (f.m.)] and C. canephora cv. Arabusta, Borbon, C. congensis, C. eugenioides, and C. canephora [16.1-21.1 g kg-1 (f.m.)].

Keywords: chlorophyll; enzyme activity; maize; Michaelis-Menten constants; total leaf proteins; Zea mays

Prepublished online: November 1, 2000; Published: August 1, 2000Show citation

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Lopez, Y., Riaño, N., Mosquera, P., Cadavid, A., & Arcila, J. (2000). Activities of Phosphoenolpyruvate Carboxylase and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in Leaves and Fruit Pericarp Tissue of Different Coffee (Coffea sp.) Genotypes. Photosynthetica, 38(2), 215-220. doi: 10.1023/A:1007265715108.
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