Photosynthetica 2014, 52(4):555-563 | DOI: 10.1007/s11099-014-0065-2

Evaluation of photosynthetic performance of wheat cultivars exposed to boron toxicity by the JIP fluorescence test

M. T. Öz1, Ö. Turan2, C. Kayihan3, F. Eyidoğan4,*, Y. Ekmekçi5, M. Yücel3, H. A. Öktem3
1 Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Molecular Biology, Palacký University, Olomouc, Czech Republic
2 Espiye Vocational School, Giresun University, Giresun, Turkey
3 Department of Biological Sciences, Middle East Technical University, Ankara, Turkey
4 Faculty of Education, Department of Elementary Education, Başkent University, Ankara, Turkey
5 Faculty of Science, Department of Biology, Hacettepe University, Ankara, Turkey

The changes in growth and photosynthetic performance of two wheat (Triticum aestivum L.) cultivars (Bolal-2973 and Atay-85) differing in their sensitivity to boron (B) toxicity were investigated under toxic B conditions. Eight-day old seedlings were exposed to highly toxic B concentrations (5, 7.5, and 10 mM H3BO3) for 5 and 9 days. Fast chlorophyll a fluorescence kinetics was determined and analysed using JIP test. Growth parameters, tissue B contents, and membrane damage were measured at two stress durations. The photochemical performance of PSII was hindered more in the sensitive cultivar (Atay-85) than that of the tolerant one (Bolal-2973) under B toxicity. The increase in the B concentration and stress duration caused membrane leakage in both cultivars. However, higher membrane damage was observed in Atay-85 compared to Bolal-2973. Additionally, significant reduction of growth parameters was observed in both cultivars at toxic B concentrations. The accumulation of B was higher in shoots than in roots of both cultivars. Nevertheless, Atay-85 translocated more B from roots to leaves compared to Bolal-2973. The advantages of certain JIP test parameters were demonstrated for evaluation of PSII activity in plants exposed to B stress. Evaluation of photosynthetic performance by JIP test as well as assessment of growth and tissue B content might be used to determine the effects of B toxicity in wheat. The results indicated lesser sensitivity to B toxicity in Bolal-2973 compared to Atay-85.

Keywords: chlorophyll fluorescence; fluorescence transient; performance index; photosynthesis

Received: October 7, 2013; Accepted: March 28, 2014; Published: December 1, 2014Show citation

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Öz, M.T., Turan, Ö., Kayihan, C., Eyidoğan, F., Ekmekçi, Y., Yücel, M., & Öktem, H.A. (2014). Evaluation of photosynthetic performance of wheat cultivars exposed to boron toxicity by the JIP fluorescence test. Photosynthetica, 52(4), 555-563. doi: 10.1007/s11099-014-0065-2.
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