Photosynthetica 2001, 39(4):489-495 | DOI: 10.1023/A:1015687525198

Photosynthetic Induction in Eucalyptus Urograndis Seedlings and Cuttings Measured by an Open Photoacoustic Cell

P.R. Barja, A.M. Mansanares, E.C. Da Silva, A.C.N. Magalhães, P.L.C.A. Alves

Photosynthetic induction in leaves of four-month-old Eucalyptus urograndis seedlings and of cuttings obtained from adult trees that were previously dark-adapted was studied by the in vivo and in situ Open Photoacoustic Cell Technique. Results for the gas exchange component of the photoacoustic (PA) signal were interpreted considering that the gas uptake component would have a phase angle nearly opposite to that of the oxygen evolution component. By subtracting the thermal component from the total PA signal, we studied the competition between gas uptake and oxygen evolution during the photosynthetic induction. Seedlings presented a net oxygen evolution prior to cuttings, but cuttings reached a higher steady-state photosynthetic activity. The chlorophyll (Chl) a/b ratio and the Chl fluorescence induction characteristic Fv/Fm were significantly higher for cuttings, while there was no difference between samples in stomata density and leaf thickness. Thus the differences in PA signals of seedlings and cuttings are associated to differences between the photosystem 2 antenna systems of these samples.

Keywords: chlorophyll fluorescence; clone differences; gas uptake; leaf thickness; oxygen evolution; photobaric signal; photothermal signal; stomata density

Published: December 1, 2001Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Barja, P.R., Mansanares, A.M., Silva, E.C., Magalhães, A.C.N., & Alves, P.L.C.A. (2001). Photosynthetic Induction in Eucalyptus Urograndis Seedlings and Cuttings Measured by an Open Photoacoustic Cell. Photosynthetica, 39(4), 489-495. doi: 10.1023/A:1015687525198.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidases in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949. Go to original source...
    2. Baesso, M.L., Mansanares, A.M., da Silva, E.C., Vargas, H., Miranda, L.C.M.: Phase-resolved photoacoustic spectroscopy and EPR investigation of MnO2 - and CuO-doped soda-lime glasses.-Phys. Rev. B 40: 1880-1884, 1989. Go to original source...
    3. Barja, P.R., Mansanares, A.M.: Photosynthetic energy storage and oxygen evolution determined through an Open Photoacoustic Cell technique.-Instrum. Sci. Technol. 26: 209-219, 1998. Go to original source...
    4. Barja, P.R., Mansanares, A.M., da Silva, B.C., Magalhães, A.C.N., Alves, P.L.C.A.: Photosynthesis in eucalyptus studied by the open photoacoustic technique: Effects of irradiance and temperature.-Acoust. Phys. 47: 16-21, 2001. Go to original source...
    5. Björkman, O.: Responses to different quantum flux densities.-In: Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H. (ed.): Physiological Plant Ecology I. Pp. 57-107. Springer-Verlag, Berlin-Heidelberg-New York 1981. Go to original source...
    6. Blake, S.T.: Four new species of Eucalyptus.-Austrobaileya 1: 7-9, 1977.
    7. Blake, T., Bevilacqua, E., Barbosa, M.M.: Early selection of fast-growing Eucalyptus clones and species.-IPEF 40: 5-14, 1988.
    8. Bults, G., Horwitz, B.A., Malkin, S., Cahen, D.: Photoacoustic measurements of photosynthetic activities in whole leaves. Photochemistry and gas exchange.-Biochim. biophys. Acta 679: 452-465, 1982. Go to original source...
    9. Buschmann, C.: Thermal dissipation during photosynthetic induction and subsequent dark recovery as measured by photoacoustic signals.-Photosynthetica 36: 149-161, 1999. Go to original source...
    10. Canaani, O., Cahen, D., Malkin, S.: Photosynthetic chromatic transitions and Emerson enhancement effects in intact leaves studied by photoacoustics.-FEBS Lett. 150: 142-146, 1982. Go to original source...
    11. Charland, M., Veeranjaneyulu, K., Charlebois, D., Leblanc, R.M.: Photoacoustic signal generation in leaves: are O2-consuming processes involved?-Biochim. biophys. Acta 1098: 261-265, 1992.
    12. da Silva, W.J., Prioli, L.M., Magalhães, A.C.N., Pereira, A.C., Vargas, H., Mansanares, A.M., Alvarado-Gil, J.: Photosynthetic O2 evolution in maize in-breds and their hybrids can be differntiated by open photo-acustic cell technique.-Plant Sci. 104: 177-181, 1995. Go to original source...
    13. Dau, H., Hansen, U.-P.: Studies on the adaptation of intact leaves to changing light intensities by a kinetic analysis of chlorophyll fluorescence and oxygen evolution as measured by the photoacoustic signal.-Photosynth. Res. 20: 59-83, 1989. Go to original source...
    14. Edwards, G., Walker, D.: C3, C4: Mechanisms, and Cellullar and Environmental Regulation of Photosynthesis.-Pp. 156-200. Blackwell Scientific Publications, Oxford 1983.
    15. Fork, D.C., Herbert, S.K.: The application of photoacoustic techniques to studies of photosynthesis.-Photochem. Photobiol. 57: 207-220, 1993. Go to original source...
    16. Gardeström, P.: Metabolite levels in the chloroplast and extrachloroplast compartments of barley leaf protoplasts during the initial phase of photosynthetic induction.-Biochim. biophys. Acta 1183: 327-332, 1993. Go to original source...
    17. Havaux, M., Canaani, O., Malkin, S.: Oxygen uptake by tobacco leaves after heat shock.-Plant Cell Environ. 10: 677-683, 1987. Go to original source...
    18. Havaux, M., Malkin, S.: Studies on photoacoustic uptake signals in tobacco leaves under high carbon dioxide levels.-Photosynth. Res. 58: 293-302, 1998. Go to original source...
    19. Inoue, M.T., Oda, S.: Photosynthesis and transpiration of cuttings and micropropagated clones of Eucalyptus grandis and grandis × E. urophylla hybrids.-Abstracts. International Symposium on Forest Tree Physiology. P. 69. Nancy 1988.
    20. Inoue, Y., Watanabe, A., Shibata, K.: Transient variation of photoacoustic signal from leaves accompanying photosynthesis.-FEBS Lett. 101: 321-323, 1979. Go to original source...
    21. Korpiun, P., Osiander, R.: Photothermal study of heat conduction, diffusion, and sorption in thin films and porous materials.-In: Bicanic, D. (ed.): Photoacoustic, Photothermal Phenomena III. Pp. 619-627. Springer-Verlag, Berlin 1992. Go to original source...
    22. Malkin, S.: Fast photoacoustic transients from dark-adapted intact leaves: oxygen evolution and uptake pulses during photosynthetic induction-a phenomenology record.-Planta 171: 65-72, 1987. Go to original source...
    23. Malkin, S., Canaani, O.: The use and characteristics of the photoacoustic method in the study of photosynthesis.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 493-526, 1994. Go to original source...
    24. Malkin, S., Puchenkov, O.: The photoacoustic effect in photosynthesis.-In: Mandelis, A., Hess, P. (ed.): Life and Earth Sciences. Pp. 17-56. SPIE Optical Engineering Press, Washington 1997.
    25. Marquezini, M.V., Cella, N., Mansanares, A.M., Vargas, H., Miranda, L.C.M.: Open Photoacoustic Cell spectroscopy.-Measur. Sci. Technol. 2: 396-401, 1991. Go to original source...
    26. Mauzerall, D.C.: Determination of oxygen emission and uptake in leaves by pulsed, time resolved photoacoustics.-Plant Physiol. 94: 278-283, 1990. Go to original source...
    27. Pereira, A.C., Neto, G. de O., Vargas, H., Cella, N., Miranda, L.C.M.: On the use of the Open Photoacoustic Cell Technique for studying photosynthetic O2 evolution of undetached leaves: comparison with Clark-type O2 electrode.-Rev. Sci. Instrum. 65: 1512-1516, 1994. Go to original source...
    28. Pereira, A.C., Zerbetto, M., Silva, G.C., Silva, W.J., Vargas, H., Neto, G. de O., Cella, N., Miranda, L.C.M.: OPC technique for in vivo studies in plant photosynthesis research.-Measur. Sci. Technol. 3: 931-934, 1992. Go to original source...
    29. Perondi, L.F., Miranda, L.C.M.: Minimal-volume photoacoustic cell measurement of thermal diffusivity: effect of the thermoelastic sample bending.-J. appl. Phys. 62: 2955-2959, 1987. Go to original source...
    30. Poulet, P., Cahen, D., Malkin, S.: Photoacoustic detection of photosynthetic oxygen evolution from leaves: quantitative analysis by phase and amplitude measurements.-Biochim. biophys. Acta 724: 433-446, 1983. Go to original source...
    31. Prinsley, R.T., Leegood, R.C.: Factors affecting photosynthetic induction in spinach leaves.-Biochim. biophys. Acta 849: 244-253, 1986. Go to original source...
    32. Pryor, L.D., Williams, E.R., Gunn, B.V.: A morphometric analysis of Eucalyptus urophylla and related taxa with descriptions of two new species.-Aust. syst. Bot. 8: 57-70, 1995. Go to original source...
    33. Rao, I.M., Terry, N.: Leaf phosphate status and photosynthesis in vivo. Changes in sugar phosphates, adenylates and nicotinamide nucleotides during photosynthetic induction in sugar beet.-Photosynthetica 30: 243-254, 1994.
    34. Reising, H., Kess, H., Price, G.D., Badger, M.R., Schreiber, U.: The main photoacoustic gas uptake signal reflects light-induced CO2-uptake associated with stroma alkalisation: new evidence based on carbonic-anhydrase-antisense transgenic tobacco plants.-Plant Cell Physiol. 39: 462-465, 1998. Go to original source...
    35. Reising, H., Schreiber, U.: Pulse-modulated photoacoustic measurements reveal strong gas-uptake component at high CO2-concentrations.-Photosynth. Res. 31: 227-238, 1992. Go to original source...
    36. Reising, H., Schreiber, U.: Inhibition by ethoxyzolamide of a photoacoustic uptake signal in leaves: Evidence for carbonic-anhydrase catalyzed CO2-solubilization.-Photosynth. Res. 42: 65-73, 1994. Go to original source...
    37. Sassenrath-Cole, G.F., Pearcy, R.W.: The role of ribulose-1,5-bisphosphate regeneration in the induction requirement of photosynthetic CO2 exchange under transient light conditions.-Plant Physiol. 99: 227-234, 1992. Go to original source...
    38. Sassenrath-Cole, G.F., Pearcy, R.W.: Regulation of photosynthetic induction state by the magnitude and duration of low light exposure.-Plant Physiol. 105: 1115-1123, 1994. Go to original source...

    Return to the content