Photosynthetica 2015, 53(2):299-305 | DOI: 10.1007/s11099-015-0114-5

Genetic basis of traits related to stomatal conductance in wheat cultivars in response to drought stress

S. G. Wang1, S. S. Jia1, D. Z. Sun1,*, H. Y. Wang1, F. F. Dong1, H. X. Ma1, R. L. Jing2,*, G. Ma3
1 College of Agronomy, Shanxi Agricultural University, Taigu, Shanxi, China
2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
3 Wheat Research Institute, Shanxi Academy of Agricultural Sciences, Linfen, Shanxi, China

The genetic basis of stomatal conductance (g s), net photosynthetic rate (P N), and transpiration rate (E) was explored by using a wheat doubled haploid population from a cross of Hanxuan10 and Lumai 14. The above three traits were evaluated in wheat flag leaves at 10, 20, 30 days after anthesis under drought stress (DS) and well-watered (WW), and quantitative trait loci (QTL) were analyzed. Expression of the traits during the grain filling stage showed downward trends under both conditions, but expression of three phenotypes were stronger under WW than those under DS. Extremely significant positive correlations were established among the traits at all growth stages under both conditions. A total of 18 additive QTLs for those traits were identified on 10 chromosomes. Among them, two batches of nine additive QTLs were associated with the target traits under DS and WW, respectively. Two additive QTLs for g s and E, two for g s and P N, six for g s, P N, and E clustered at the same or near the region (colocation) of chromosomes 4A, 2B, and 7B, respectively. This provided genetic basis for close phenotype correlations among g s, P N, and E. Furthermore, QTLs for g s, P N, and E near Xgwm577 and Xgwm611 located on 7B chromosome were linked to previously reported QTLs regulating a SPAD value and the chlorophyll a/b ratio under dark-induced condition. This finding indicated that these QTLs on 7B chromosome might be involved in the process of wheat leaf senescence.

Keywords: drought stress; photosynthetic rate; quantitative trait loci; stomatal conductance; transpiration rate; wheat

Received: February 26, 2014; Accepted: September 2, 2014; Published: June 1, 2015Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Wang, S.G., Jia, S.S., Sun, D.Z., Wang, H.Y., Dong, F.F., Ma, H.X., Jing, R.L., & Ma, G. (2015). Genetic basis of traits related to stomatal conductance in wheat cultivars in response to drought stress. Photosynthetica, 53(2), 299-305. doi: 10.1007/s11099-015-0114-5.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Casson S., Gray J.E.: Influence of environmental factors on stomatal development. - New Phytol. 178: 9-23, 2008. Go to original source...
    2. Fischer R.A., Rees D., Sayre K.D. et al.: Wheat yield progress associated with higher stomatal conductance and photosynthetic rate, and cooler canopies. - Crop Sci. 38: 1467-1475, 1998. Go to original source...
    3. Franks P.J., Drake P.L., Beerling D.J.: Plasticity in maximum stomatal conductance constrained by negative correlation between stomatal size and density: an analysis using Eucalyptus globulus. - Plant Cell Environ. 32: 1737-1748, 2009. Go to original source...
    4. Gillon J.S., Yakir D.: Internal Conductance to CO2 diffusion and C18OO discrimination in C3 leaves. - Plant Physiol. 123: 201-214, 2000. Go to original source...
    5. Hao Z.F., Chang X.P., Guo X.J. et al.: QTL mapping for drought tolerance at stages of germination and seedling in wheat (Triticum aestivum L.) using a DH population. - Agric. Sci. China 2: 943-949, 2003.
    6. Hervé D., Fabre F., Berrios E.F. et al.: QTL analysis of photosynthesis and water status traits in sunflower (Helianthus anuus L.) under greenhouse conditions. - J. Exp. Bot. 362: 1857-1864, 2001.
    7. Hetherington A.M., Woodward F.I.: The role of stomata in sensing and driving environmental change. - Nature 424: 901-908, 2003. Go to original source...
    8. Jing R., Chang X., Jia J. et al.: Establishing wheat doubled haploid population for genetic mapping by anther culture. - Biotechnology 9: 4-8, 1999.
    9. Li H., Lin F., Wang G. et al.: Quantitative trait loci mapping of dark-induced senescence in winter wheat (Triticum aestivum). - J. Integr. Plant Biol. 54: 33-44, 2012. Go to original source...
    10. Lu J.Y., Shan L., Gao J.F. et al.: Effect of drought stress on photosynthesis and some other physiological characteristics in flag leaf during grain filling of wheat. - Agr. Res. Arid Areas 21: 77-81, 2003.
    11. Miyashita K., Tanakamaru S., Maitani T. et al.: Recovery responses of photosynthesis, transpiration, and stomatal conductance in kidney bean following drought stress. - Environ. Exp. Bot. 53: 205-214, 2005. Go to original source...
    12. Shimshi D., Ephrat J.: Stomatal behavior of wheat cultivars in relation to their transpiration, photosynthesis, and yield. - Agron. J. 67: 326-331, 1975. Go to original source...
    13. Veldboom L.R., Lee M., Woodman W.L.: Molecular marker-facilitated studies in an elite maize population: I. Linkage analysis and determination of QTL for morphological traits. - Theor. Appl. Genet. 88: 7-16, 1994. Go to original source...
    14. Vijayalakshmi K., Fritz A.K., Paulsen G.M. et al.: Modeling and mapping QTL for senescence-related traits in winter wheat under high temperature. - Mol. Breeding 26: 163-175, 2010.
    15. Wang D.L., Zhu J., Li Z.K. et al.: Mapping QTLs with epistatic effects and QTL×environment interactions by mixed linear model approaches. - Theor. Appl. Genet. 99: 1255-1264, 1999. Go to original source...
    16. Yang H.M., Wang G.X.: Leaf stomatal densities and distribution in Triticum Aestivum under drought and CO2 enrichment. - Acta Phytoecol. Sin. 25: 312-316, 2001.
    17. Yu S.B., Li J.X., Xu C.G. et al.: Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid. - P. Natl. Acad. Sci. USA 94: 9226-9231, 1997. Go to original source...
    18. Zhang Y.Q., Lin Q., Liu J.B. et al.: Effects of drought stress on photosynthetic characteristics and yield of different fertilizer and water types of wheat. - J. Triticeae Crops 31: 724-730, 2011.
    19. Zhao X.Q., Xu J.L., Zhao M. et al.: QTLs affecting morph-physiological traits related to drought tolerance detected in overlapping introgression lines of rice (Oryza sativa L.). - Plant Sci. 174: 618-625, 2008. Go to original source...
    20. Zheng T.C., Zhang X.K., Yin G.H. et al.: Genetic gains in grain yield, net photosynthesis and stomatal conductance achieved in Henan Province of China between 1981 and 2008. - Field Crop. Res. 122: 225-233, 2011. Go to original source...
    21. Zhou X.G., Jing R.L., Hao Z.F. et al.: Mapping QTL for seedling root traits in common wheat. - Sci. Agric. Sin. 38: 1951-1957, 2005.

    Return to the content