Photosynthetica 2019, 57(1):332-341 | DOI: 10.32615/ps.2019.038

Identification of a GLDH-overexpressing Arabidopsis mutant and its responses to high-light stress

X.T. ZHENG, X.H. ZHANG, Y.Z. WANG, M.L. CAI, M. LI, T.J. ZHANG, C.L. PENG
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, School of Life Sciences, South China Normal University, Guangzhou 510631, China

Ascorbic acid (AsA) is an important antioxidant protecting plant against environmental stresses. L-galactono-1,4-lactone dehydrogenase (GLDH) is a key enzyme in AsA synthesis pathway. To investigate the roles of AsA in mitigating high light (HL) damage, Arabidopsis GLDH mutants SALK_060087 and SALK_008236 with altered GLDH-expression were screened for homozygotes. No homozygotes were identified from SALK_060087, but most individuals of the SALK_008236 line (GLDH-236OE) were GLDH-overexpressing homozygous mutants accumulating more AsA than wild type (WT). An investigation of the physiological responses to HL demonstrated that the chlorophyll fluorescence parameters were significantly higher in GLDH-236OE than that in WT after 14-d HL. The degradation of photosynthetic pigment in WT was more severe than that in GLDH-236OE. GLDH-236OE accumulated more AsA, anthocyanins, flavonoids, and phenolics, while WT accumulated more reactive oxygen species (ROS) during HL. Our results suggest that GLDH-236OE have lesser sensitivity and higher tolerance to HL due to a higher capacity to eliminate ROS, absorb extra light, and dissipate thermal energy.

Keywords: abiotic stress; antioxidation; ascorbate; photoinhibition; photoprotection.

Received: May 22, 2018; Accepted: October 15, 2018; Prepublished online: December 20, 2018; Published: January 30, 2019Show citation

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ZHENG, X.T., ZHANG, X.H., WANG, Y.Z., CAI, M.L., LI, M., ZHANG, T.J., & PENG, C.L. (2019). Identification of a GLDH-overexpressing Arabidopsis mutant and its responses to high-light stress. Photosynthetica, 57(1), 332-341. doi: 10.32615/ps.2019.038.
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