Photosynthetica 2018, 56(1):418-426 | DOI: 10.1007/s11099-017-0754-8

Response of photosynthetic apparatus in Arabidopsis thaliana L. mutant deficient in phytochrome A and B to UV-B

V. D. Kreslavski1,2,*, A. N. Shmarev1, V. Yu. Lyubimov1, G. A. Semenova3, S. K. Zharmukhamedov1,2, G. N. Shirshikova1, A. Yu. Khudyakova1,2, S. I. Allakhverdiev1,2,4,5,6,*
1 Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, Russia
2 Controlled Photobiosynthesis Laboratory, Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia
3 Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia
4 Department of Plant Physiology, Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia
5 Institute of Molecular Biology and Biotechnology, Azerbaijan National Academy of Sciences, Baku, Azerbaijan
6 Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia

The effects of UV-B radiation (1 W m-2, 1 and 2 h) on PSII activity, chloroplast structure, and H2O2 contents in leaves of 26-d-old Arabidopsis thaliana phyA phyB double mutant (DMut) compared with the wild type (WT) were investigated. UV-B decreased PSII activity and affected the H2O2 content in WT and DMut plants grown under white light (WL). The chloroplast structure changes in DMut plants exposed to UV were more significant than that in WT. Reductions in maximal and real quantum photochemical yields and increase in the value of thermal dissipation of absorbed light energy per PSII RC and the amount of QB-nonreducing centers of PSII were bigger in mutant compared to WT plants grown both under WL and red light. Such difference in action of UV-B on WT and DMut can be explained by higher content of UV-absorbing pigments and carotenoids in WT leaves compared with DMut.

Keywords: chloroplast structure; fluorescence of Chl a; photosystem II; phytochrome mutants; stress; ultraviolet

Received: May 18, 2017; Accepted: August 11, 2017; Published: March 1, 2018Show citation

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Kreslavski, V.D., Shmarev, A.N., Yu. Lyubimov, V., Semenova, G.A., Zharmukhamedov, S.K., Shirshikova, G.N., Yu. Khudyakova, A., & Allakhverdiev, S.I. (2018). Response of photosynthetic apparatus in Arabidopsis thaliana L. mutant deficient in phytochrome A and B to UV-B. Photosynthetica, 56(1), 418-426. doi: 10.1007/s11099-017-0754-8.
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