Photosynthetica 2013, 51(4):509-516 | DOI: 10.1007/s11099-013-0049-7

Effects of addition of external nitric oxide on the allocation of photosynthetic electron flux in Rumex K-1 leaves under osmotic shock

H. D. Li1,3, W. B. Wang1, P. M. Li2, K. Xu3, H. Y. Gao1,*, J. Xiao4
1 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, P.R. China
2 College of Horticulture, Northwest A&F University, Yangling, Shanxi, PR China
3 College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, P.R. China
4 Agrotechnical Station, Agricultural Bureau of Daiyue District, Tai'an, Shandong, P.R. China

Photosynthetic electron flux allocation, stomatal conductance, and the activities of key enzymes involved in photosynthesis were investigated in Rumex K-1 leaves to better understand the role of nitric oxide (NO) in photoprotection under osmotic stress caused by polyethylene glycol. Gas exchange and chlorophyll fluorescence were measured simultaneously with a portable photosynthesis system integrated with a pulse modulated fluorometer to calculate allocation of photosynthetic electron fluxes. Osmotic stress decreased stomatal conductance, photosynthetic carbon assimilation, and nitrate assimilation, increased Mehler reaction, and resulted in photoinhibition. Addition of external NO enhanced the stomatal conductance, photosynthetic rate, activities of glutamine synthetase and nitrate reductase, and reduced Mehler reaction and photoinhibition. These results demonstrated that osmotic stress reduced CO2 assimilation, decreasing the use of excited energy via CO2 assimilation which caused significant photoinhibition. Improving stomatal conductance by the addition of external NO enhanced the use of excited energy via CO2 assimilation. As a result, less excited energy was allocated to Mehler reaction, which reduced production of reactive oxygen species via this pathway. We suppose that Mehler reaction is not promoted unless photosynthesis and nitrogen metabolism are prominently inhibited.

Keywords: osmotic stress; photoenergy allocation

Received: October 10, 2012; Accepted: February 28, 2013; Published: December 1, 2013Show citation

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Li, H.D., Wang, W.B., Li, P.M., Xu, K., Gao, H.Y., & Xiao, J. (2013). Effects of addition of external nitric oxide on the allocation of photosynthetic electron flux in Rumex K-1 leaves under osmotic shock. Photosynthetica, 51(4), 509-516. doi: 10.1007/s11099-013-0049-7.
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