Photosynthetica 2018, 56(4):1365-1369 | DOI: 10.1007/s11099-018-0840-6

Viscosimetric analysis of the mechanism of ATP hydrolysis by pea chloroplast F1-ATPase

A.N. Malyan1,*
1 Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, Russia

Dependence of ATP hydrolysis kinetics by the chloroplast coupling factor (CF1) on medium viscosity was studied at varying temperatures. For samples with oxidized and reduced CF1 γ-subunit, this dependence was shown to be described by Cramers' relationship k ~ (η/ηo)-n, where k is the reaction rate constant, η/ηo is the medium/water viscosity ratio, and 0 < n < 1. Transition of the γ-subunit from its reduced to oxidized state was accompanied by increasing n value, which is indicative of increasing friction losses between certain enzyme sections and the solution. The increased medium viscosity produced no effect on the reaction activation energy which appeared to be almost the same for the both enzyme states. The molecular mechanisms responsible for CF1 activity loss in viscous media are discussed.

Keywords: ATP hydrolysis; F1-ATPase; kinetics; viscosity

Received: December 1, 2017; Accepted: March 9, 2018; Prepublished online: December 1, 2018; Published: November 1, 2018Show citation

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Malyan, A.N. (2018). Viscosimetric analysis of the mechanism of ATP hydrolysis by pea chloroplast F1-ATPase. Photosynthetica, 56(4), 1365-1369. doi: 10.1007/s11099-018-0840-6.
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