Photosynthetica 2016, 54(1):56-64 | DOI: 10.1007/s11099-015-0155-9

Natural and commercial Salix clones differ in their ecophysiological response to Zn stress

A. Bernardini1,*, E. Salvatori1, S. Di Re1, L. Fusaro1, G. Nervo2, F. Manes1
1 Department of Environmental Biology, Sapienza University of Rome, Rome, Italy
2 Agricultural Research Council - Intensive Wood Production Research Unit (CRA-PLF), Casale Monferrato (AL), Italy

This study was carried out to determine the effect of different zinc concentrations on the ecophysiological response of Salix clones: four commercial clones ("1962", "1968", "Drago", and "Levante") selected for short rotation coppice, and one natural clone, "Sacco", obtained from a contaminated area. Gas exchanges, chlorophyll a fluorescence (JIP-test), relative chlorophyll content, and biometric parameters were measured in plants grown for fifteen days in soil containing Zn concentrations of 0, 300, 750, and 1,500 mg(ZnCl2) kg-1. Ecophysiological response to metal stress differed in dependence on the Zn concentration and clone. At the low Zn concentration (300 mg kg-1), the absence of any significant reductions in parameters investigated indicated an efficient plant homeostasis to maintain the metal content within phytotoxic limits. Stomatal limitation, observed at 750 and 1,500 mg kg-1, which was found in all clones after three days of the treatment, might be caused by indirect effects of metal on guard cells. Among commercial clones, "Drago" was more sensitive to Zn stress, showing inhibition of growth, while "1962" clone showed a downregulation of PSII photochemistry following the slowdown in the Calvin-Benson cycle. On the contrary, the natural Salix clone ("Sacco") performed better compared to the other clones due to activation of a photosynthetic compensatory mechanism.

Keywords: chlorophyll a fluorescence; gas exchange; stress response; willow; zinc

Received: December 22, 2014; Accepted: May 15, 2015; Published: March 1, 2016Show citation

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Bernardini, A., Salvatori, E., Re, S., Fusaro, L., Nervo, G., & Manes, F. (2016). Natural and commercial Salix clones differ in their ecophysiological response to Zn stress. Photosynthetica, 54(1), 56-64. doi: 10.1007/s11099-015-0155-9.
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