Photosynthetica 2004, 42(3):345-349 | DOI: 10.1023/B:PHOT.0000046151.97327.b2

Modulation in vivo by Nitrate Salts of the Activity and Properties of Phosphoenolpyruvate Carboxylase in Leaves of Alternanthera pungens (C4 plant) and A. sessilis (C3 species)

A.V. Rajagopalan1, R.M. Agarwal2, A.S. Raghavendra1
1 University of Hyderabad, India
2 School of Studies in Botany, Jiwaji University, India

Feeding K+ or Na+ nitrate salts in vivo enhanced the activity of phosphoenolpyruvate carboxylase (PEPC) in the leaf extracts of Alternanthera pungens (C4 plant) and A. sessilis (C3 species). The increase was more pronounced in A. pungens than in A. sessilis. Chloride salts increased the PEPC activity only marginally. However, the sulfate salts were either not effective or inhibitory. Feeding nitrate modulated the regulatory properties of PEPC in A. pungens, resulting in increased KI (malate) and decreased KA (glucose-6-P). The sensitivity of PEPC to malate, which gives a measure of phosphorylation status of the enzyme, indicated that feeding leaves with NO3- enhanced the phosphorylation status of the enzyme. The reduction in PEPC activity due to cycloheximide treatment suggested that increased synthesis of PEPC protein kinase may be one of the reasons for the enhancement in PEPC activity, after the nitrate feeding. We suggest that nitrate salts could be used as a tool to modulate and analyze the properties of PEPC in C3 and C4 plants.

Keywords: cycloheximide; glucose-6-phosphate; inorganic salts; L-malate; nitrate; phosphorylation; regulation; sulphate

Published: September 1, 2004Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Rajagopalan, A.V., Agarwal, R.M., & Raghavendra, A.S. (2004). Modulation in vivo by Nitrate Salts of the Activity and Properties of Phosphoenolpyruvate Carboxylase in Leaves of Alternanthera pungens (C4 plant) and A. sessilis (C3 species). Photosynthetica, 42(3), 345-349. doi: 10.1023/B:PHOT.0000046151.97327.b2.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Andreo, C.S., Gonzalez, D.H., Iglesias, A.A.: Higher plant phosphoenolpyruvate carboxylase. Structure and regulation.-FEBS Lett. 213: 1-8, 1987. Go to original source...
    2. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphe-noloxidase in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949. Go to original source...
    3. Brownell, P.F.: Sodium as an essential micronutrient element for plants and its possible role in metabolism.-Adv. bot. Res. 7: 117-224, 1979.
    4. Chollet, R., Vidal, J., O'Leary, M.H.: Phosphoenolpyruvate car-boxylase: A ubiquitous, highly regulated enzyme in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 273-298, 1996. Go to original source...
    5. Duff, S.M.G., Chollet, R.: In vivo regulation of wheat-leaf phos-phoenolpyruvate carboxylase by reversible phosphorylation.-Plant Physiol107: 775-782, 1995. Go to original source...
    6. Gupta, S.K., Ku, M.S.B., Lin, J.-H., Zhang, D., Edwards, G.E.: Light/dark modulation of phosphoenolpyruvate carboxylase in C3 and C4 plants.-Photosynth. Res. 42: 133-143, 1994. Go to original source...
    7. Jensen, W.A., Armstrong, J.M., DeGiorgio, J., Hearn, M.T.: Stability studies on maize leaf phosphoenolpyruvate carboxylase: the effect of salts.-Biochemistry 34: 472-480, 1995. Go to original source...
    8. Jiao, J.A., Chollet, R.: Posttranslational regulation of phos-phoenolpyruvate carboxylase in C4 and Crassulacean acid me-tabolism plants.-Plant Physiol. 95: 981-985, 1991. Go to original source...
    9. Li, B., Zhang, X.-Q., Chollet, R.: Phosphoenolpyruvate carbo-xykinase in tobacco leaves is activated by light in a similar but not identical way as in maize.-Plant Physiol. 111: 497-505, 1996. Go to original source...
    10. Manh, C.T., Bismuth, E., Boutin, J.P., Provot, M., Champigny, M.L.: Metabolite effectors for short-term nitrogen dependent enhancement of phosphoenolpyruvate carboxylase activity and decrease of net sucrose synthesis in wheat leaves.-Physiol. Plant. 89: 460-466, 1993. Go to original source...
    11. Miyao, M., Fukayama, H.: Metabolic consequences of over-production of phosphoenolpyruvate carboxylase in C3 plants.-Arch. Biochem. Biophys. 414: 197-203, 2003. Go to original source...
    12. Parvathi, K., Gayathri, J., Maralihalli, G.B., Bhagwat, A.S., Raghavendra, A.S.: Modulation of phosphoenolpyruvate car-boxylase phosphorylation in leaves of Amaranthus hypo-chondriacus, a NAD-ME type of C4 plant.-Photosynthetica 38: 23-28, 2000. Go to original source...
    13. Rajagopalan, A.V., Devi, M.T., Raghavendra, A.S.: Molecular biology of C4 phosphoenolpyruvate carboxylase: Structure, regulation and genetic engineering.-Photosynth. Res. 39: 115-135, 1994. Go to original source...
    14. Sedmak, J.J., Grossberg, S.E.: A rapid, sensitive and versatile assay for protein using comassie brilliant blue G 250.-Anal. Biochem. 79: 544-552, 1977. Go to original source...
    15. Sugiharto, B., Miyata, K. Nakamoto, H., Sasakawa, H., Sugiyama, T.: Regulation of expression of carbon assimilating enzymes by nitrogen in maize leaf.-Plant Physiol. 92: 963-969, 1990. Go to original source...
    16. Sugiharto, B., Sugiyama, T.: Effects of nitrate and ammonium on gene expression of phosphoenolpyruvate carboxylase and nitrogen metabolism in maize leaf tissue during recovery from nitrogen stress.-Plant Physiol. 98: 1403-1408, 1992. Go to original source...
    17. Sugiharto, B., Suzuki, I., Burnell, J.N., Sugiyama, T.: Glutamine induces the N-dependent accumulation of mRNAs encoding phosphoenolpyruvate carboxylase and carbonic an-hydrase in detached maize leaf tissue.-Plant Physiol. 100: 2066-2070, 1992. Go to original source...
    18. Van Quy, L., Champigny, M.L.: NO3-enhances the kinase acti-vity for phosphorylation of phophoenolpyruvate carboxylase and sucrose phosphate synthase proteins in wheat leaves.-Plant Physiol. 99: 344-347, 1992. Go to original source...
    19. Van Quy, L., Foyer, C., Champigny, M.L.: Effects of light and NO3-on wheat leaf phosphoenolpyruvate carboxylase activity. Evidence for covalent modulation of the C3 enzyme.-Plant Physiol. 97: 1476-1482, 1991. Go to original source...
    20. Wyn Jones, R.G., Pollard, A.: Proteins, enzymes and inorganic ions.-In: Lauchli, A., Bieleski, R.L. (ed.): Inorganic Plant Nutrition. Pp. 528-562. Springer-Verlag, Berlin 1983.

    Return to the content