Institute of Experimental Medicine CAS

Neurorecon

About Project – News – Project Duration – Research Team – Publications – Contacts – Fotogallery

About Project

Center of Reconstruction Neuroscience – NEURORECON
CZ.02.1.01/0.0/0.0/15_003/0000419

Project duration

1. 1. 2017 – 30. 10. 2022

eu-msmt

The project Center of Reconstruction Neuroscience – NEURORECON encompasses the creation and development of excellent research team headed by prominent foreign scientists, prof. James Fawcett (University of Cambridge), which will deal with R & D in the field of therapeutic methods nervous system. The project builds on existing R & D activities of the Institute of Experimental Medicine ASCR, v.v.i. and in connection with its implementation will strengthen the quality of research at the international level, the acquisition of lack of infrastructures and intensify international cooperation IEM.

The project is financed from the Operational Programme Research and Education (OP VVV), which is a multi-year thematic program sponsored by the Ministry of Education, Youth and Sports, within which it is possible in the programming period 2014-2020 draw on funds from the European Structural and Investment Funds (ESI +).

News

Newsletters - březen 2017

2017

Regeneration, Plasticity, Protection Conference (prof. James Fawcett) - 10. - 11. 2017, Prague - Conference Programme
Two-day workshop - speakers from JOHN VAN GEEST CENTRE FOR BRAIN REPAIR, which is a part of the Department of Clinical Neuroscience (University of Cambridge)

DRG INJECTION WORKSHOP (Dr. Menghon Cheah)
Perineuronal net treatments for memory loss (Dr. Sujeong Yang)

Training workshop on DRG isolation in situ in the cervical and lumbar spine accompanied by practical examples
Co-operation with Dr. Vincenzo de Paola from Imperial College London (in vivo imaging of organotypic incisions) and Dr. Diego Peretti from University of Cambridge (hibernation model)
Dr. Růžička took part in 3-month internship at Imperial College London (Faculty of Medicine, Institute of Clinical Sciences – under the leadership of Dr. Vincenzo de Paola)
Lecture by prof. Toshitaka Oohashi - The Hyaluronan and Proteoglycan Link Proteins: Organizers of the Brain ECM and Key Molecules for Neuronal Function and Plasticity
Mgr. Dubišová took part in internship at University of Cambridge
Participation of the project members at TERMIS EU 2017 Congress in Tissue Engineering and Regenerative Medicine in Switzerland, organization of symposium "New trends in CNS repair" within the congress, three lectures and two poster announcements
Co-operation with the group of dr. Andras Lakatos from University of Cambridge, lecture on Regulatory networks controlling protective and detrimental astrocyte phenotypes in injury and ALS.
During the year, the Center's workplace was gradually built; investment equipment was acquired, including the Lightsheet Microscope

2018

Lecture by Dr. Elisabeth Bradbury (King's College London) - Restoring function after spinal cord injury: targeting glial scar matrix and endogenous repair processes
Lecture by Dr. Kristian Franze (Department of Physiology, Development and Neuroscience, University of Cambridge) - The mechanical regulation of neuronal development and regeneration
Lecture by prof. Joost Verhaagen (Netherlands Institute for Neuroscience) - Neuronal regeneration: from gene networks to gene therapy
Lecture by prof. Britta Eickholt (Charité – Universitätsmedizin Berlin) – Injury induced drebrin controls astrogliosis and scar formation by regulating tubular endosomes and adhesion responses
Mgr. Neumannová took part in 3-day workshop on Application of viral vectors into spinal ganglia in Netherlands Institute for Neuroscience, Amsterdam
Mgr. Hahnová took part in 3-month internship at Institute for Molecular and Cell Biology, University of Porto (under the leadership of Dr. Joao Relvas)
Controlling neuronal plasticity - developmental disorders and repair Conference in Vila Lanna, Prague - http://cnp-2018.com/content/programme

Research Team

prof. MUDr. James Fawcett, Ph.D.
doc. RNDr. Pavla Jendelová, Ph.D.
PharmDr. Šárka Kubinová, Ph.D.
Dr. Jessica Kwok, Ph.D.
MUDr. Lucia Machová Urdziková, Ph.D.
Dr. Slaven Erceg, Ph.D.
RNDr. Jiří Růžička, Ph.D.
Mgr. Jana Svobodová Burianová, Ph.D.
Mgr. Kristýna Kárová, Ph.D.
Mgr. Kristýna Kekulová
Mgr. Monika Řehořová
Mgr. Jana Vallová
Mgr. Kateřina Neumannová
Mgr. Noelia Martinez Varea
Mgr. Klára Hahnová
Mgr. Ingrid Vargová

External advisory team

Dr. Elisabeth Bradbury – King's College London
prof. Joost Verhaagen – Netherlands Institute for Neuroscience, Amsterdam
Ing. Miroslava Anděrová, PhD. – ÚEM AV ČR
Doc. MVDr. Daša Čížková, DrSc – Ústav Imunologie, SAV, Bratislava
Dr. Andreas Lakatos – University of Cambridge

Publications

2017

Foscarin, S., Raha-Chowdhury, R., Fawcett, J., Kwok, J.: (2017) Brain ageing changes proteoglycan sulfation, rendering perineuronal nets more inhibitory. Aging. 9 (6): 1607-1622.
Koseki, H., Donegá, M., Lam, B.Y.H., Petrová, V., van Erp, S., Yeo, G.S.H., Kwok, J., Ffrench-Constant, Ch., Eva, R., Fawcett, J.: (2017) Selective rab11 transport and the intrinsic regenerative ability of CNS axons. eLife. 6: e26956.
Yang, S., Hilton, S., Alves, J.N., Saksida, L.M., Bussey, T., Matthews, R.T., Kitagawa, H., Spillantini, M.G., Kwok, J., Fawcett, J.: (2017) Antibody recognizing 4-sulfated chondroitin sulfate proteoglycans restores memory in tauopathy-induced neurodegeneration. Neurobiology of Aging. 59: 197-209.
Giulia E. Tyzack, Claire E. Hall, Christopher R. Sibley, Tomasz Cymes, Serhiy Forostyak, Giulia Carlino, Ione Meyer, Giampietro Schiavo, Su-Chun Zhang, George M. Gibbons, Jia Newcombe, Rickie Patani & András Lakatos*A neuroprotective astrocyte st 1 ate is induced by neuronal signal EphB1 but fails in ALS models, Nature Communications, Oct 27;8(1):1164.
Lucia Machova Urdzikova L, Ruzicka J,Karova K, Kloudova A, Svobodova B, Amin A, Dubisova J, Schmidt M, Kubinova S, Jhanwar-Uniyal M, Jendelova P. A green tea polyphenol epigallocatechin-3-gallate enhances neuroregeneration after spinal cord injury by altering levels of inflammatory cytokines, Neuropharmacology, 2017 Nov;126:213-223. doi: 10.1016/j.neuropharm.2017.09.006. https://doi.org/10.1016/j.neuropharm.2017.09.006
Jiri Ruzicka, Lucia Machova Urdzikova, Barbora Svobodova, Anubhav G.Amin, Kristyna Karova, Jana Dubisova, Kristyna Zaviskova, Sarka Kubinova, Meic Schmidt,Meena Jhanwar-Uniyal and Pavla Jendelova Combined therapy of Curcumin and Epigallocatechin gallate in an experimental model of spinal cord injury in rats. Neural Regeneration Research (in press)
Serhiy Forostyak* & Eva Sykova, Neuroprotective potential of cell-based therapies in ALS: from bench to bedside, Front Neurosci. 2017 Oct 24;11:591. doi: 10.3389/fnins.2017.00591. eCollection 2017
Eva R, Koseki H, Kanamarlapudi V, Fawcett JW. EFA6 regulates selective polarised transport and axon regeneration from the axon initial segment. J Cell Sci. 2017 Nov 1;130(21):3663-3675. doi: 10.1242/jcs.207423. Epub 2017 Sep 21.
Cheah M, Fawcett JW, Andrews MR. Assessment of Thermal Pain Sensation in Rats and Mice Using the Hargreaves Test. Bio Protoc. 2017 Aug 20;7(16). pii: e2506. doi: 10.21769/BioProtoc.2506.
Richter RP, Baranova NS, Day AJ, Kwok JC. Glycosaminoglycans in extracellular matrix organisation: are concepts from soft matter physics key to understanding the formation of perineuronal nets? Curr Opin Struct Biol. 2017 Dec 21;50:65-74. doi: 10.1016/j.sbi.2017.12.002.

2018

Nieuwenhuis B, Haenzi B, Andrews MR, Verhaagen J, Fawcett JW. Integrins promote axonal regeneration after injury of the nervous system. Biol Rev CambPhilos Soc. 2018 Feb 15. doi: 10.1111/brv.12398. [Epub ahead of print] PubMed PMID: 29446228.
Krupa P, Vackova I, Ruzicka J, Zaviskova K, Dubisova J, Koci Z, Turnovcova K, Urdzikova LM, Kubinova S, Rehak S, Jendelova P. The Effect of Human Mesenchymal Stem Cells Derived from Wharton's Jelly in Spinal Cord Injury Treatment Is Dose-Dependent and Can Be Facilitated by Repeated Application. Int J Mol Sci. 2018 May 17;19(5). pii: E1503. doi: 10.3390/ijms19051503.
Ruzicka J, Urdzikova LM, Svobodova B, Amin AG, Karova K, Dubisova J, Zaviskova K, Kubinova S, Schmidt M, Jhanwar-Uniyal M, Jendelova P. Does combined therapy of curcumin and epigallocatechin gallate have a synergistic neuroprotective effect against spinal cord injury? Neural Regen Res. 2018 Jan;13(1):119-127. doi: 10.4103/1673-5374.224379.
Artero Castro A, Lukovic D, Jendelova P, Erceg S. Concise Review: Human Induced Pluripotent Stem Cell Models of Retinitis Pigmentosa. Stem Cells. 2018 Apr;36(4):474-481. doi: 10.1002/stem.2783. Epub 2018 Feb 7.
Hejcl A, Ruzicka J, Proks V, Mackova H, Kubinova S, Tukmachev D, Cihlar J, Horak D, Jendelova P. Dynamics of tissue ingrowth in SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores after bridging a spinal cord transection. J Mater Sci Mater Med. 2018 Jun 25;29(7):89. doi: 10.1007/s10856-018-6100-2.
Fawcett JW. The Paper that Restarted Modern Central Nervous System Axon Regeneration Research. Trends Neurosci. 2018 May;41(5):239-242. doi: 10.1016/j.tins.2018.02.012.
Irvine SF, Kwok JCF. Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones. Int J Mol Sci. 2018 Apr 12;19(4). pii: E1172. doi: 10.3390/ijms19041172.
Warren PM, Dickens SM, Gigout S, Fawcett JW, Kwok JCF. Regulation of CNS plasticity through the extracellular matrix. Oxford Handbook of Developmental Neural Plasticity. Subject: Neuroscience, Development of the Nervous System. DOI: 10.1093/oxfordhb/9780190635374.013.11. Online Publication Date: Oct 2018
Fawcett JW, Verhaagen J. Intrinsic Determinants of Axon Regeneration. Dev Neurobiol. 2018 Oct;78(10):890-897. doi: 10.1002/dneu.22637. Epub 2018 Oct 21.
Rowlands D, Lensjø KK, Dinh T, Yang S, Andrews MR, Hafting T, Fyhn M, Fawcett JW, Dick G. Aggrecan directs extracellular matrix mediated neuronal plasticity. J Neurosci. 2018 Nov 21;38(47):10102-10113. doi: 10.1523/JNEUROSCI.1122-18.2018. Epub 2018 Oct 3.
Hejcl A, Ruzicka J, Kekulova K, Svobodova B, Proks V, Mackova H, Jirankova K, Karova K, Machova Urdzikova L, Kubinova S, Cihlar J, Horak D, Jendelova P. Modified methacrylate hydrogels improve tissue repair after spinal cord injury. Int J Mol Sci. 2018 Aug 22;19(9). pii: E2481. doi: 10.3390/ijms19092481.
Jendelova P, Sykova E, Erceg S. Neural Stem Cells Derived from Human-Induced Pluripotent Stem Cells and Their Use in Models of CNS Injury. Results Probl Cell Differ. 2018;66:89-102. doi: 10.1007/978-3-319-93485-3_3.
Karova, K., Wainwright, J.V, Machova-Urdzikova, L., Rishikaysh Pisal, R.V., Schmidt, M., Jendelova, P., Jhanwar-Uniyal, M. Transplantation of neural precursors generated from spinal progenitor cells reduces inflammation in spinal cord injury via NF-κB pathway inhibition. J Neuroinflammation. 2019 Jan 17;16(1):12. doi: 10.1186/s12974-019-1394-7.
Ruzicka J, Romanyuk N, Jirakova K, Hejcl A, Janouskova O, Urdzikova Machova L, Bochin M, Pradny M, Vargova L, Jendelova P. The effect of iPS derived neural progenitors seeded on laminin coated pHEMA-MOETACl hydrogel with dual porosity, in a rat model of chronic spinal cord injury. Cell Transplant. 2019 Jan 18:963689718823705. doi: 10.1177/0963689718823705. [Epub ahead of print]