22.1. 2019, Translační metabolismus

PhD project: Application of new stationary phases (HPLC, CE) for separation of biologically important metabolites

The analysis of biologically important metabolites is important task for not only practically oriented analytical chemistry. The list of these important compounds is really broad and still increasing. The physico-chemical properties of these substances are often very different and therefore we must often use different separation systems to separate them. Nevertheless, the division of these compounds is often unsuccessful. Therefore, both specific and nonspecific separation systems, stationary phases for HPLC as well as CE (capillary electrophoresis), need to be developed. In this project, stationary phases will be developed at collaborating institutes, aiming to apply them to realistic analyzes.

Candidate’s profile (requirements):

We are seeking outstanding self-motivated candidates with master's degree or equivalent in analytical chemistry, biochemistry, or those expecting to obtain their degree this year. Candidates should read and speak in English. Experience with analytical methods, mainly chromatography and capillary electrophoresis, are advantage.

Relevant publications:

Statis Pataridis, Oleg Romanov, Ivan Mikšík: Identification of short-chain poly-3-hydroxybutyrates in Saiga horn extracts using LC-MS/MS. Jourmal of Separation Science, in press, 2019. (https://doi.org/10.1002/jssc.201800910)

Jana Svobodova; Olga Kofronová; Oldrich Benada; Vladimír Král; Ivan Mikšík: Separation of oligopeptides, nucleobases, nucleosides and nucleotides using capillary electrophoresis/electrochromatography with sol-gel modified inner capillary wall. Journal of Chromatography A, 1517:185-194, 2017.

David Sýkora, Václav Kašička, Ivan Mikšík, Pavel Řezanka, Kamil Záruba, Pavel Matějka, Vladimír Král: Application of gold nanoparticles in separation sciences, Journal of Separation Science, 33(3): 372-387, 2010

Supervisor: Ivan Mikšík, Prof., D.Sc.