Photosynthetica 2018, 56(1):294-299 | DOI: 10.1007/s11099-018-0795-7

Determination of PS I oligomerisation in various cyanobacterial strains and mutants by non-invasive methods

T. Zakar1, L. Kovacs1, S. Vajravel1, E. Herman1, M. Kis1, H. Laczko-Dobos1, Z. Gombos1,*
1 Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary

PSI trimer to monomer ratio in intact cyanobacterial cells and isolated thylakoids was analysed by two noninvasive, in vivo methods; low-temperature fluorescence emission and circular dichroism spectroscopy. We measured fluorescence emission spectra of cells upon chlorophyll (Chl, 436 nm) excitation. All three species - Synechocystis sp. PCC 6803, Anabaena sp. PCC 7120, and Spirulina platensis - showed shifted Chl peak, indicating they have different spectral properties. CD spectroscopy revealed the highest intensity at 515 nm (PSI peak) in Spirulina platensis cells, which may originate from PSI multi-oligomerisation. The most sensitive response to heat treatment in this strain was the oligomerisation of PSI RCs. PSI dimers and tetramers in Anabaena cells showed smaller changes of the CD signal upon the heat treatment compared to that of Synechocystis WT. The lack of γ-linolenic acid affected the filament morphology by the loss of the spiral shape and the PSI monomerisation in Spirulina I22.

Keywords: carotenoids; pigment-protein interactions; temperature stress; xanthophylls

Received: June 26, 2017; Accepted: February 19, 2018; Published: March 1, 2018Show citation

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Zakar, T., Kovacs, L., Vajravel, S., Herman, E., Kis, M., Laczko-Dobos, H., & Gombos, Z. (2018). Determination of PS I oligomerisation in various cyanobacterial strains and mutants by non-invasive methods. Photosynthetica, 56(1), 294-299. doi: 10.1007/s11099-018-0795-7.
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    References

    1. Bautista J.A., Rappaport F., Guergova-Kuras M. et al.: Biochemical and biophysical characterization of photosystem I from phytoene desaturase and zeta-carotene desaturase deletion mutants of Synechocystis sp. PCC 6803: evidence for PsaA- and PsaB-side electron transport in cyanobacteria.-J. Biol. Chem. 280: 20030-20041, 2005. Go to original source...
    2. Chitnis P.R.: Photosystem I.-Plant Physiol. 111: 661-669, 1996. Go to original source...
    3. Chitnis P.R.: Photosystem I: Function and Physiology.-Annu. Rev. Plant Phys. 52: 593-626, 2001. Go to original source...
    4. Chitnis V.P., Chitnis P.R.: PsaL subunit is required for the formation of photosystem I trimers in the cyanobacterium Synechocystis sp. PCC 6803.-FEBS Lett. 336: 330-334, 1993. Go to original source...
    5. Chitnis V.P., Xu Q., Yu L., Golbeck J.H. et al.: Targeted inactivation of the gene psaL encoding a subunit of photosystem I of the cyanobacterium Synechocystis sp. PCC 6803.-J. Biol. Chem. 268: 11678-11684, 1993.
    6. Domonkos I., Kis M., Gombos Z. et al.: Carotenoids, versatile components of oxygenic photosynthesis.-Prog. Lipid Res. 52: 539-561, 2013. Go to original source...
    7. El-Mohsnawy E., Kopczak M.J., Schlodder E. et al.: Structure and function of intact photosystem 1 monomers from the cyanobacterium Thermosynechococcus elongatus.-Biochemistry 49: 4740-4751, 2010. Go to original source...
    8. Fromme P., Jordan P., Krauss N.: Structure of photosystem I.-BBA-Bioenergetics 1507: 5-31, 2001. Go to original source...
    9. Garab G., van Amerongen H.: Linear dichroism and circular dichroism in photosynthesis research.-Photosynth. Res. 101: 135-146, 2009. Go to original source...
    10. Gobets B., van Grondelle R.: Energy transfer and trapping in photosystem I.-Biochim. Biophys. Acta. 1507: 80-99, 2001. Go to original source...
    11. Golbeck J.H.: Structure and function of Photosystem I.-Annu. Rev. Plant Phys. 43: 293-324, 1992. Go to original source...
    12. Guskov A., Kern J., Gabdulkhakov A. et al.: Cyanobacterial photosystem II at 2.9-A resolution and the role of quinones, lipids, channels and chloride.-Nat. Struct. Mol. Biol. 16: 334-342, 2009.
    13. Jordan P., Fromme P., Witt H.T. et al.: Three-dimensional structure of cyanobacterial photosystem I at 2.5 A resolution.-Nature 411: 909-917, 2001. Go to original source...
    14. Karapetyan N.V: Protective dissipation of excess absorbed energy by photosynthetic apparatus of cyanobacteria: role of antenna terminal emitters.-Photosynth. Res. 97: 195-204, 2008. Go to original source...
    15. Karapetyan N.V., Dorra D., Schweitzer G. et al.: Fluorescence spectroscopy of the longwave chlorophylls in trimeric and monomeric photosystem I core complexes from the cyanobacterium Spirulina platensis.-Biochemistry 36: 13830-13837, 1997. Go to original source...
    16. Karapetyan N.V., Holzwarth A.R., Rogner M.: The photosystem I trimer of cyanobacteria: molecular organization, excitation dynamics and physiological significance.-FEBS Lett. 460: 395-400, 1999. Go to original source...
    17. Kłodawska K., Kovács L., Várkonyi Z. et al.: Elevated growth temperature can enhance photosystem I trimer formation and affects xanthophyll biosynthesis in Cyanobacterium Synechocystis sp. PCC 6803 cells.-Plant Cell Physiol. 56: 558-571, 2015. Go to original source...
    18. Komenda J., Barber J.: Comparison of psbO and psbH deletion mutants of Synechocystis PCC 6803 indicates that degradation of D1 protein is regulated by the QB site and dependent on protein synthesis.-Biochemistry 34: 9625-9631, 1995. Go to original source...
    19. Kusama Y., Inoue S., Jimbo H. et al.: Zeaxanthin and Echinenone protect the repair of photosystem II from Inhibition by singlet oxygen in Synechocystis sp. PCC 6803.-Plant Cell Physiol. 56: 906-916, 2015. Go to original source...
    20. Prentki P., Krisch M.H.: In vitro insertional mutagenesis with a selectable DNA fragment.-Gene 29: 303-313, 1984. Go to original source...
    21. Rippka R., Deruelles J., Waterbury J. et al.: Generic assignments, strain histories and properties of pure cultures of cyanobacteria.-J. Gen. Microbiol. 111: 1-61, 1979. Go to original source...
    22. Schäfer L., Vioque A., Sandmann G.: Functional in situ evaluation of photosynthesis-protecting carotenoids in mutants of the cyanobacterium Synechocystis PCC 6803.-J. Photoch. Photobio. B 78: 195-201, 2005. Go to original source...
    23. Schluchter W.M., Shen G., Zhao J. et al.: Characterization of psaI and psaL mutants of Synechococcus sp. strain PCC 7002: a new model for state transitions in cyanobacteria.-Photochem. Photobiol. 64: 53-66, 1996. Go to original source...
    24. Shubin V.V., Tsuprun V.L., Bezsmertnaya I.N. et al.: Trimeric forms of the photosystem I reaction center complex pre-exist in the membranes of the cyanobacterium Spirulina platensis.-FEBS Lett. 334: 79-82, 1993. Go to original source...
    25. Sozer O., Komenda J., Ughy B. et al.: Involvement of carotenoids in the synthesis and assembly of protein subunits of photosynthetic reaction centers of Synechocystis sp. PCC 6803.-Plant Cell Physiol. 51: 823-835, 2010. Go to original source...
    26. Takaichi S., Mochimaru M.: Carotenoids and carotenogenesis in cyanobacteria: unique ketocarotenoids and carotenoid glycosides.-Cell Mol. Life Sci. 64: 2607-2619, 2007. Go to original source...
    27. Tian L., van Stokkum I.H., Koehorst R.B. et al.: Site, rate, and mechanism of photoprotective quenching in cyanobacteria.-J. Am. Chem. Soc. 133: 18304-18311, 2011. Go to original source...
    28. Tóth T.N., Chukhutsina V., Domonkos I. et al.: Carotenoids are essential for the assembly of cyanobacterial photosynthetic complexes.-Biochim. Biophys. Acta. 1847: 1153-1165, 2015.
    29. Trissl H.W.: Long-wavelength absorbing antenna pigments and heterogeneous absorption bands concentrate excitons and increase absorption cross section.-Photosynth. Res. 35: 247-263, 1993. Go to original source...
    30. Turconi S., Kruip J., Schweitzer G. et al: A comparative fluorescence kinetics study of photosystem I monomers and trimers from Synechocystis PCC 6803.-Photosynth. Res. 49: 263-268, 1996. Go to original source...
    31. Vajravel S., Kis M., Klodawska K. et al.: Zeaxanthin and echinenone modify the structure of photosystem I trimer in Synechocystis sp. PCC 6803.-BBA-Bioenergetics 1858: 510-518, 2017.
    32. van Grondelle R., Dekker J.P., Gillbro T. et al.: Energy transfer and trapping in photosynthesis.-BBA-Bioenergetics 1187: 1-65, 1994. Go to original source...
    33. Watanabe M., Semchonok D.A., Webber-Birungi M.T. et al.: Attachment of phycobilisomes in an antenna-photosystem I supercomplex of cyanobacteria.-P. Natl. Acad. Sci. USA 111: 2512-2517, 2014. Go to original source...
    34. Xu J., Ramian G.J., Galan J.F. et al.: Terahertz circular dichroism spectroscopy: a potential approach to the in situ detection of life's metabolic and genetic machinery.-Astrobiology 3: 489-504, 2003. Go to original source...
    35. Zakar T., Herman E., Vajravel S. et al.: Lipid and carotenoid cooperation-driven adaptation to light and temperature stress in Synechocystis sp. PCC 6803.-Biochim. Biophys. Acta 1858: 337-350, 2017.

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