Photosynthetica 2015, 53(4):562-571 | DOI: 10.1007/s11099-015-0144-z

Assessing the relationship between photosynthetic C accumulation and symbiotic N nutrition in leaves of field-grown nodulated cowpea (Vigna unguiculata L. Walp.) genotypes

A. K. Belane1, F. D. Dakora2,*
1 Department of Crop Sciences, Tshwane University of Technology, Pretoria, South Africa
2 Chemistry Department, Tshwane University of Technology, Pretoria, South Africa

This study evaluated the relationship between photosynthetic carbon accumulation and symbiotic nitrogen nutrition in young fully expanded leaves of 30 nodulated cowpea genotypes grown in the field at Manga, Ghana, in 2005 and 2006. Estimates of fixed-N in photosynthetic leaves revealed greater symbiotic N in genotypes with higher photosynthetic rates and increased leaf transpiration rate/efficiency. There was also greater C accumulation in genotypes with higher symbiotic N and/or total N. Additionally, genotypes with high contents of C per unit of leaf total N exhibited greater C per unit of leaf N-fixed. The C/N and C/Rubisco-N ratios were generally similar in their magnitude when compared to the C/N-fixed ratio due possibly to the fact that Rubisco accounts for a high proportion of photosynthetic leaf N, irrespective of whether the enzyme was formed from soil N or symbiotic N. Cowpea genotypes that relied heavily on soil N for their N nutrition exhibited much higher C/N-fixed ratios, while conversely those that depended more on symbiosis for meeting their N demands showed markedly lower C/N-fixed values. For example, genotypes Omondaw, Bensogla, IT93K-2045-29, and Sanzie, which respectively derived 83.9, 83.1, 82.9, and 76.3% N from fixation, recorded lower C/N-fixed ratios of 10.7, 12.2, 12.1, and 13.0 mg mg-1 in that order in 2005. In contrast, genotypes Botswana White, IT94D-437-1, TVu1509, and Apagbaala, which obtained 14.8, 15.0, 26.4, and 26.0% of their N nutrition from fixation, showed high C/N-fixed values of 84.0, 69.0, 35.2, and 40.6 mg.mg-1, respectively, in 2005. This clearly indicates that genotypes that obtained less N from symbiosis and more N from soil revealed very high C/N-fixed values, an argument that was reinforced by the negative correlations obtained between the three C/N ratios (i.e. C/N, C/Rubisco-N, and C/N-fixed) and leaf N concentration, percentage nitrogen derived from fixation, total N content, amount of N-fixed, and Rubisco N. These data suggest a direct link between photosynthetic C accumulation and symbiotic N assimilation in leaves of nodulated cowpea, and where genotypes derived a large proportion of their N from fixation, photosynthetic C yield substantially increased.

Keywords: N2 fixation; photosynthesis; photosynthetic fixed-N use efficiency; transpiration efficiency; δ15N

Received: July 11, 2014; Accepted: March 25, 2015; Published: December 1, 2015Show citation

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Belane, A.K., & Dakora, F.D. (2015). Assessing the relationship between photosynthetic C accumulation and symbiotic N nutrition in leaves of field-grown nodulated cowpea (Vigna unguiculata L. Walp.) genotypes. Photosynthetica, 53(4), 562-571. doi: 10.1007/s11099-015-0144-z.
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