Photosynthetica 2010, 48(3):339-347 | DOI: 10.1007/s11099-010-0044-1

Changes in photosynthesis, fluorescence, and nitrogen metabolism of hawthorn (Crataegus pinnatifida) in response to exogenous glutamic acid

C. Yu1,2, D. G. Lv1,*, S. J. Qin1, L. Yang1, H. Y. Ma1, G. C. Liu1
1 College of Horticulture, Shenyang Agricultural University, Shenyang, P.R. China
2 Cash Crops Institute, Hubei Academy of Agricultural sciences, Wuhan, P.R. China

Photosynthesis, chlorophyll (Chl) a fluorescence, and nitrogen metabolism of hawthorn (Crataegus pinnatifida Bge.), subjected to exogenous L-glutamic acid (GLA) (200 mg l-1, 400 mg l-1, and 800 mg l-1) that possibly affect secondary metabolic regulation, were measured. The results indicated that photosynthetic and fluorescence characteristics of hawthorn exhibited positive responses to the application of GLA. Different concentrations of GLA caused an increase in Chl content, net photosynthetic rate (P N) and stomatal conductance (g s) as well as transpiration rate (E), and improved the carboxylation efficiency (CE), apparent quantum yield (AQY) and maximum carboxylation velocity of Rubisco (Vcmax). Application of GLA could also enhance the maximum ratio of quantum yields of photochemical and concurrent non-photochemical processes in PSII (Fv/F0), the maximal quantum yield of PSII (Fv/Fm), the probability that an absorbed photon will move an electron into the electron transport chain beyond QA (ΦEo) as well as the performance index on absorption basis (PIABS), but decreased the intercellular CO2 concentration (C i) and the minimal fluorescence (F0). Application of GLA also induced an increase in nitrate reductase (NR; EC 1.6.6.1) and glutamine synthetase (GS; EC 6.3.1.2) activities, and increased the soluble protein content, leaf nitrogen (N) content and N accumulation in leaves as well as the plant biomass. However, the effects were different among different concentrations of GLA, and 800 mg l-1 GLA was better. This finding suggested that application of GLA is recommended to improve the photosynthetic capacity by increasing the light energy conversion and CO2 transfer as well as the photochemical efficiency of PSII, and enhanced the nitrogen metabolism and growth and development of plants.

Keywords: chlorophyll fluorescence; glutamine synthetase; leaf nitrogen content; net photosynthetic rate; nitrate reductase; soluble protein content; stomatal conductance; transpiration rate

Received: January 22, 2009; Accepted: April 29, 2010; Published: September 1, 2010Show citation

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Yu, C., Lv, D.G., Qin, S.J., Yang, L., Ma, H.Y., & Liu, G.C. (2010). Changes in photosynthesis, fluorescence, and nitrogen metabolism of hawthorn (Crataegus pinnatifida) in response to exogenous glutamic acid. Photosynthetica, 48(3), 339-347. doi: 10.1007/s11099-010-0044-1.
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