Photosynthetica 2002, 40(2):301-304 | DOI: 10.1023/A:1021366228791

Dissipation of Excitation Energy During Development of Photosystem 2 Photochemistry in Helianthus annuus

J.G. Lebkuecher1, L.E. Altmon1, G.K. Harris1, K.L. Wallace1, A.R. Wilding1
1 Department of Biology, Austin Peay State University, Clarksville, USA, e-mail

Etiolated sunflower cotyledons developed in complete darkness and lacking photosystem (PS) 2 were exposed to continuous 200 µmol(photon) m-2 s-1 "white light" for 1, 3, 6, 12, and 18 h prior to evaluations of excitation-energy dissipation using modulated chlorophyll a fluorescence. Photochemical potential of PS2, measured as the dark-adapted quantum efficiency of PS2 (FV(M)/FM), and thermal dissipation from the antenna pigment-protein complex, measured as the Stern-Volmer non-photochemical quenching coefficient (NPQ), increased to 12 h of irradiation. Following 12 h of irradiation, thermal dissipation from the antennae pigment-protein complex decreased while the efficiency of excitation capture by PS2 centers (F'V/F'M) and light-adapted quantum efficiency of PS2 (ΦPS2) continued to increase to 18 h of irradiation. The fraction of the oxidized state of QA, measured by the photochemical quenching coefficient (qP), remained near optimal and was not changed significantly by irradiation time. Hence during the development of maximum photochemical potential of PS2 in sunflower etioplasts, which initially lacked PS2, enhanced thermal dissipation helps limit excitation energy reaching PS2 centers. Changes of the magnitude of thermal dissipation help maintain an optimum fraction of the oxidized state of QA during the development of PS2 photochemistry.

Keywords: chlorophyll a fluorescence; etioplast; non-photochemical dissipation; sunflower

Published: June 1, 2002Show citation

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Lebkuecher, J.G., Altmon, L.E., Harris, G.K., Wallace, K.L., & Wilding, A.R. (2002). Dissipation of Excitation Energy During Development of Photosystem 2 Photochemistry in Helianthus annuus. Photosynthetica, 40(2), 301-304. doi: 10.1023/A:1021366228791.
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    References

    1. Chow, W.S., Funk, C., Hope, A.B., Govindjee: Greening of intermittent-light-grown bean plants in continuous light: thylakoid components in relation to photosynthetic performance and capacity for photoprotection.-Indian J. Biochem. Biophys. 37: 395-404, 2000.
    2. Deltoro, V.I., Calatayud, A., Morales, F., Abadia, A., Barreno, E.: Changes in net photosynthesis, chlorophyll fluorescence and xanthophyll cycle interconversions during freeze-thaw cycles in the Mediterranean moss Leucodon sciuroides.-Oecologia 120: 499-505, 1999. Go to original source...
    3. Demmig-Adams, B., Adams, W.W., III: Photoprotection and other responses of plants to high light stress.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 599-626, 1992. Go to original source...
    4. Demmig-Adams, B., Adams, W.W., III, Barker, D.H., Logan, B.A., Bowling, D.R., Verhoeven, A.S.: Using chlorophyll fluorescence to assess the fraction of absorbed light allo-cated to thermal dissipation of excess excitation.-Physiol. Plant. 98: 253-264, 1996.
    5. Eickmeier, W.G., Lebkuecher, J.G., Osmond, C.B.: Photosynthetic water oxidation and water stress in plants.-In: Somero, G.N., Osmond, C.B., Bolis, C.L. (ed.): Water and Life: Comparative Water Relationships at the Organismic, Cellular, and Molecular Levels. Pp. 223-239. Springer-Verlag, Berlin 1992. Go to original source...
    6. Franck, F., Schoefs, B., Barthélemy, X., Myśliwa-Kurdziel, B., Strzałka, K., Popovic, R.: Protection of native chlorophyll(ide) forms and of photosystem II against photodamage during early stages of chloroplast differentiation.-Acta Physiol. Plant. 17: 123-132, 1995.
    7. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    8. Gilmore, A.M., Govindjee: How higher plants respond to excess light: energy dissipation in photosystem II.-In: Singhal, G.S., Renger, G., Sopory, S.K., Irrgang, K.-D., Govindjee (ed.): Concepts in Photobiology. Pp. 513-548. Narosa House, Delhi-Madras-Bombay-Calcutta-Lon-don 1999. Go to original source...
    9. Gilmore, A.M., Hazlett, T.L., Govindjee: Xanthophyll cycle-dependent quenching of photosystem II chlorophyll a fluorescence: formation of a quenching complex with a short fluorescence lifetime.-Proc. nat. Acad. Sci. USA 92: 2273-2277, 1995. Go to original source...
    10. Jahns, P., Krause, G.H.: Xanthophyll cycle and energy-dependent fluorescence quenching in leaves from pea plants grown under intermittent light.-Planta 192: 176-182, 1994. Go to original source...
    11. Johnson, G., Krieger, A.: Thermoluminescence as a probe of Photosystem II in intact leaves: Non-photochemical fluorescence quenching in peas grown in an intermittent light regime.-Photosynth. Res. 41: 371-379, 1994. Go to original source...
    12. Laisk, A., Oja, V., Rasulov, B., Eichelmann, H., Sumberg, A.: Quantum yields and rate constants of photochemical and non-photochemical excitation quenching. Experiment and model.-Plant Physiol. 115: 803-815, 1997. Go to original source...
    13. Lebkuecher, J.G.: Desiccation-time limits of photosynthetic recovery in Equisetum hyemale (Equisetaceae) spores.-Amer. J. Bot. 84: 792-797, 1997. Go to original source...
    14. Lebkuecher, J.G., Haldeman, K.A., Harris, C.E., Holz, S.L., Joudah, S.A., Minton, D.A.: Development of photosystem-II activity during irradiance of etiolated Helianthus (Asteraceae) seedlings.-Amer. J. Bot. 86: 1087-1092, 1999. Go to original source...
    15. Mullet, J.E.: Chloroplast development and gene expression.-Annu. Rev. Plant Physiol. Plant mol. Biol. 39: 475-502, 1988. Go to original source...
    16. Mysliwa-Kurdziel, B., Barthélemy, X., Strzalka, K., Franck, F.: The early stages of Photosystem II assembly monitored by measurements of fluorescence lifetime, fluorescence induction and isoelectric focusing of chlorophyll-proteins in barley etiochloroplasts.-Plant Cell Physiol. 38: 1187-1196, 1997. Go to original source...
    17. Ögren, E.: Prediction of photoinhibition of photosynthesis from measurements of fluorescence quenching components.-Planta 184: 538-544, 1991. Go to original source...
    18. Ohashi, K., Tanaka, A., Tsuji, H.: Formation of the photosynthetic electron transport system during the early phase of greening in barley leaves.-Plant Physiol. 91: 409-414, 1989. Go to original source...
    19. Schreiber, U., Bilger, W., Neubauer, C.: Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis.-In: Schulze, E.-D., Caldwell, M.M. (ed.): Ecophysiology of Photosynthesis. Pp. 49-70. Springer-Verlag, Berlin 1994. Go to original source...
    20. Šiffel, P., Hunalová, I., Roháček, K.: Light-induced quenching of chlorophyll fluorescence at 77 K in leaves, chloroplasts and Photosystem II particles.-Photosynth. Res. 65: 219-229, 2000.
    21. Sokal, R.R., Rohlf, F.J.: Biometry.-W.H. Freeman, New York 1995.
    22. Srivastava, A., Greppin, H., Strasser, R.J.: The steady state chlorophyll a fluorescence exhibits in vivo an optimum as a function of light intensity which reflects the physiological state of the plant.-Plant Cell Physiol. 36: 839-848, 1995. Go to original source...
    23. Sundberg, B., Campbell, D., Palmquist, K.: Predicting CO2 gain and photosynthetic light acclimation from fluorescence yield and quenching in cyano-lichens.-Planta 201: 138-145, 1997. Go to original source...
    24. Webber, A.N., Baker, N.R.: Control of thylakoid membrane development and assembly.-In: Ort, D.R., Yocum, C.F. (ed.): Oxygenic Photosynthesis: the Light Reactions. Pp. 41-58. Kluwer Acad. Publ., Dordrecht-Boston-London 1996. Go to original source...
    25. Zar, J.E.: Biostatistical Analysis.-Englewood Cliffs, New Jersey 1984.

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