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We study the lateral organization of biological membranes into functional microdomains with the emphasis on their fine structure, dynamics and molecular principles of formation. Morphological changes or disintegration of these cellular structures are usually accompanied by the pathologic phenotypes. Taking the maximum advantage from the genetically accessible yeast model, the research at the Microscopy Unit is focused on the involvement of membrane microdomains in the stress perception and adaptation, signaling and regulation of metabolic processes. Recently we described the model of general membrane structure re-organization in response to membrane depolarization as induced by environmental stimuli, pathogen or stress exposure.
Petra Veselá, MSc
E-mail: petra.vesela@iem.cas.cz
Tel.: +420 241 062 119
Assoc. Prof. Jan Malínský, PhD.
Jakub Zahumenský, MSc, PhD.
Petra Veselá, MSc
Thuraya Awadová, MSc (ML)
Katarína Vaškovičová, MSc
Lenka Doudová
Jitka Eisensteinová
Dagmar Folková, MSc
Lenka Hlavínová (ML)
Martin Oškera, MSc
Evolutionarily conserved 5´-3´exoribonuclease Xrn1 binds to eisosome
under conditions of glucose deprivation. Association of Xrn1 with the plasma membrane represents a novel mechanism of the mRNA decay regulation. Subcellular Xrn1 localization also reflects the nutrient supply and metabolic differentiation within the yeast colony. Central section of the whole 5 days old colony of S. cerevisiae expressing Xrn1-GFP (A), stratification of specific Xrn1-GFP patterns (B), and large magnification details (C) of cells showing Xrn1 distributed in cytosol (1), accumulated in P-bodies (2) and associated with eisosome (3) are presented. Fluorescence Recovery After Photobleaching (FRAP) analysis revealed that in contrast to P-body binding, the enzyme association with the plasma membrane at eisosome is stable in time (D). Bars: 300 μm (A), 10 μm (C).
Publication:
Vaškovičová, K., Awadová, T., Veselá, P. , Balážová, M., Opekarová, M., Malínský, J.: (2017) mRNA decay is regulated via the spatial sequestration of the conserved 5 '-3 ' exoribonuclease Xrn1 at a specific microdomain of the yeast plasma membrane. European Journal of Cell Biology. 96(6): 591-599. IF 3,712.
Kubalová D, Káňovičová P, Veselá P, Awadová T, Džugasová V, Daum G, Malínský J, Balážová M.:(2019) The lipid droplet protein Pgc1 controls the subcellular distribution of phosphatidylglycerol. FEMS Yeast Res. 2019 Aug 1;19(5). pii: foz045. doi: 10.1093/femsyr/foz045.
Pivoňková, H. , Heřmanová, Z. , Kirdajová, D. , Awadová, T., Malínský, J., Valihrach, L., Žucha, D., Kubista, M., Gálisová, A., Jirák, D., Anděrová, M.: (2018) The Contribution of TRPV4 Channels to Astrocyte Volume Regulation and Brain Edema Formation. Neuroscience. 2018 Dec 1;394:127-143. doi: 10.1016/j.neuroscience.2018.10.028. Epub 2018 Oct 24.
Awadová, T ., Pivoňková, H., Heřmanová, Z., Kirdajová, D., Anděrová, M., Malínský, J.: (2018) Cell volume changes as revealed by fluorescence microscopy: Global vs local approaches. J Neurosci Methods. 2018 Aug 1;306:38-44. doi: 10.1016/j.jneumeth.2018.05.026. Epub 2018 Jun 7.
Vaškovičová, K., Awadová, T., Veselá, P. , Balážová, M., Opekarová, M., Malínský, J.: (2017) mRNA decay is regulated via the spatial sequestration of the conserved 5 '-3 ' exoribonuclease Xrn1 at a specific microdomain of the yeast plasma membrane. European Journal of Cell Biology. 96(6): 591-599.
Chum, T., Glatzová, D., Kvíčalová, Z., Malínský, J., Brdička, T., Cebecauer, M.: (2016) The role of palmitoylation and transmembrane domain in sorting of transmembrane adaptor proteins. J. Cell Sci., 129(1): 95-107.
Malínský, J., Opekarová, M.: (2016) New Insight Into the Roles of Membrane Microdomains in Physiological Activities of Fungal Cells. Int. Rev. Cell Mol. Biol., 325: 119-180.
Malínský, J., Tanner, W., Opekarová, M.: (2016) Transmembrane voltage: Potential to induce lateral microdomains. Acta Mol. Cell Biol. Lipids, 1861(8 Pt B): 806-811.
Plecitá-Hlavatá, L., Engstová, H., Alán, L., Špaček, T., Dlasková, A., Smolková, K., Špačková, J., Tauber, J., Strádalová, V., Malínský, J., Lessard, M., Bewersdorf, J., Ježek, P.: (2016) Hypoxic HepG2 cell adaptation decreases ATP synthase dimers and ATP production in inflated cristae by mitofilin down-regulation concomitant to MICOS clustering. Faseb J., 30(5): 1941-1957.
Pokorná, L., Čermáková, P., Horváth, A., Baile, M.G., Claypool, S.M., Griač, P., Malínský, J., Balážová, M.: (2016) Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function. Biochim. Biophys. Acta-Bioenerg., 1857(1): 34-45.
Wang, H.X., Douglas, L.M., Veselá, P., Rachel, R., Malinský, J., Konopka, J.B.: (2016) Eisosomes promote the ability of Sur7 to regulate plasma membrane organization in Candida albicans. Mol Biol Cell., 27(10): 1663-1675.
Grousl, T., Opekarová, M., Strádalová, V., Hašek, J., Malínský, J.: (2015) Evolutionarily Conserved 5'-3' Exoribonuclease Xrn1 Accumulates at Plasma Membrane-Associated Eisosomes in Post-Diauxic Yeast. PLoS One. 10(3): e0122770.
Herman, P., Večeř, J., Opekarová, M., Veselá, P., Jančíková, I., Zahumenský, J., Malinský, J.: (2015) Depolarization affects lateral microdomain structure of yeast plasma membrane. FEBS J. 282(3): 419-434.
Pokorná, L., Čermáková, P., Horváth, A., Baile, M.G., Claypool, S.M., Griač, P., Malínský, J., Balážová, M.: (2015) Specific degradation of phosphatidylglycerol is necessary for proper mitochondrial morphology and function. Biochim Biophys Acta. 1857(1): 34-45.
Vaškovičová, K., Strádalová, V., Efenberk, A., Opekarová, M., Malínský, J.: (2015) Assembly of fission yeast eisosomes in the plasma membrane of budding yeast: Import of foreign membrane microdomains. Eur. J. Cell Biol. 94(1): 1-11.
Vecer, J., Veselá, P., Malínský, J., Herman, P.: (2014) Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast. FEBS Lett. 588(3): 443-449.
Malínský, J., Opekarová, M., Grossmann, G., Tanner, W.: (2013) Membrane microdomains, rafts, and detergent-resistant membranes in plants and fungi. Annu. Rev. Plant Biol. 64: 501-529.
Rinnerthaler, M., Slaba, R., Grousl, T., Strádalová, V., Heeren, G., Richter, K., Breitenbach-Koller, H., Malínský, J., Hasek, J., Breitenbach, M.: (2013) Mmi1, the yeast homologue of mammalian TCTP associates with stress granules in Heat-Shocked Cells and Modulates proteasome activity. PLoS One. 8(10): e77791.
SAV-18-25 Barth syndrome - Study of mitochondrial morphology in a novel yeast model, 2018-2020
19-04052S Function of specific membrane microdomains in lipid homeostasis, 2019-2021
20-04987S The role of eisosome in the regulation of stress response, 2020-2022
Institute of Animal Biochemistry and Genetics SAS
University of Regensburg
Institute of Physics of Charles University
Stony Brook University
Prague Membrane Discussions (PMD)
Informal scientific discussions focused on cellular membranes (not only).
https://praguemembranediscussions.weebly.com/
The Summer Event will take place at IOCB in Dejvice (May or June).
Spring PMD 2018
March 8th, 2018 ; 10 am - 2 pm
@ Jaroslav Heyrovsky Institute of the CAS, Dolejskova 3, Prague 8
Brdicka lecture room
Program:
10:00 - 11:00 Plenary lecture 1.
Claudia Steinem - Universitaet Goettingen, Germany
Pore-spanning membranes: Is this model system suited for addressing biochemical questions?
11:00 - 12:00 Plenary lecture 2.
Andreas Janshoff - Universitaet Goettingen, Germany
Membrane biophysics in confined geometry
12:00-12:30 COFFEE/SNACKS BREAK (drinks, coffee and snacks available)
12:30 – 13:15
Robert Vacha, MU Brno
Translocation of peptides across phospholipid membranes
13:15 – 14:00
Petra Riegrova, JHI
The oxidized phospholipids modify Bax protein ability to induce permeabilization of mitochondrial membranes
2017
Poslední PMD v roce 2017 se uskutečnilo ve čtvrtek 14. 12. a hostitelem byl tentokrát Ústav experimentální botaniky AV ČR.
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