Photosynthetica - Ahead of Print
Special issue in honour of Prof. Reto J. Strasser – Development and aging of photosynthetic apparatus of Vitis vinifera L. during growing season
K. SITKO, S. RUSINOWSKI, M. POGRZEBA, A. DASZKOWSKA-GOLEC, Ż. GIEROŃ, H.M. KALAJI, E. MAŁKOWSKI
Photosynthetica X:X | DOI: 10.32615/ps.2019.107
The aim of this study was to examine the development and aging of chosen grapevine leaves in situ during the growing season (130 d) using chlorophyll (Chl) a fluorescence measurements and determining the changes in pigment contents. During the course of photosystems development, the increase of Chl and decrease of anthocyanin contents in leaves was observed simultaneously. On 28th day, the maximum content of Chl and minimum content of anthocyanins was measured. However, the maximal photosynthetic performance was found one week later, when the content of Chl started to diminish. Our study proved that the achievement of maximal...
Special issue in honour of Prof. Reto J. Strasser – Plant biomass in salt-stressed young maize plants can be modelledwith photosynthetic performance
V. GALIĆ, M. MAZUR, D. ŠIMIĆ, Z. ZDUNIĆ, M. FRANIĆ
Photosynthetica X:X | DOI: 10.32615/ps.2019.131
Predicting responses to stressful conditions is very important. Chlorophyll a fluorescence (ChlF) can be used to assess effects of various stresses on photosynthetic performance. We tested the responses of five 10-d old maize hybrids to salinity stress by measuring ChlF parameters, fresh (FM) and dry mass (DM). ChlF data were incorporated into a penalized regression model to predict biomass traits. The values of FM and DM significantly decreased under salt stress by 42 and 25%, respectively. Strong responses in ChlF parameters assessing the absorption dissipation and trapping fluxes to NaCl treatment were detected. In penalized regression models,...
Special issue in honour of Prof. Reto J. Strasser – Structural and functional response of photosynthetic apparatus of radish plants to iron deficiency
I.A. SAMBORSKA-SKUTNIK, H.M. KALAJI, L. SIECZKO, W. BĄBA
Photosynthetica X:X | DOI: 10.32615/ps.2019.132
In this work, we tried to identify some specific chlorophyll a fluorescence (ChlF) parameters, that could enable detection of iron deficiency (Fedef) in radish plants (Raphanus sativus L.), before any visual symptoms appear. Changes in ChlF kinetics, JIP-test parameters, and chlorophyll content revealed that iron deficiency negatively affected PSII activity mainly via disruption of light absorption in light-harvesting complexes and by decreasing the activity of the primary quinone acceptor of PSII (QA). Iron deficiency was clearly reflected in the changes of some JIP-test parameters, such as time to reach...
Photosynthetic characteristics and chlorophyll a fluorescence induction parameters in elite clone (GS1) of Pinus sylvestris var. mongolica
P. MENG, G.C. YOU
Photosynthetica X:X | DOI: 10.32615/ps.2019.133
We selected the elite clone of Pinus sylvestris var. mongolica 'GS1' from 365 clones in a primary seed orchard in Liaoning Province, China. To understand the photosynthesis characteristics and mechanisms, 40-year-old 'GS1' trees were compared with a common clone 'H524' of the same age at the same seed orchard. Results showed that 'GS1' had higher net photosynthetic rate because of higher stomatal conductance (gs); the larger external and inner diameters of stomata were the structural factors enabling maintenance of higher gs. The higher light-saturated net photosynthetic rate of 'GS1' was mainly due...
Leaf photosynthetic light response of summer maize: comparison of models and analysis of parameters
T.T. ZHU, J. LI, Y.Y. LIU, X.J. TONG, Q. YU
Photosynthetica X:X | DOI: 10.32615/ps.2019.134
An experiment was performed in order to study the applicability of light-response models for summer maize (Zea mays L.) by using rectangular hyperbola model, nonrectangular hyperbola model, exponential model, binomial regression model, and modified rectangular hyperbola model (Ye model), respectively. Our results showed that the fitted effect of Ye model was best and photosynthetic parameters were closest to the observation. The photoinhibition coefficient was significantly negatively correlated with the light-saturation point (Is), the light-saturation coefficient (γ), and maximum net photosynthetic rate at light-saturation...
Inhibition of photosynthesis in cucumber leaves by oxybenzone
X. ZHONG, Y.T. LI, Z.S. ZHANG, S.H. LI, Y.M. LI, B.B. LIU, Q.M. LI, H.Y. GAO
Photosynthetica X:X | DOI: 10.32615/ps.2019.135
Oxybenzone (OBZ), a popular additive in sunscreen, can protect human skin by blocking ultraviolet (UV) rays from the sun (Baker et al. 2015). In addition to being used in sunscreens, OBZ is also widely used in perfumes, shampoos, lip balms, and other pharmaceuticals and personal care products (PPCPs) (Aguirre et al. 1992, Emonet et al. 2001, Kasichayanula et al. 2007). OBZ can enter rivers, lakes, groundwater, sewage, and so on through human activities and was identified as an 'emerging environmental pollutant' by the Environmental Protection Agency of the USA (Rodil et al. 2012, Agüera et al. 2013). With...
Ultraviolet (UV) B effects on growth and yield of three contrasting sweet potato cultivars
Z. CHEN, W. Gao, K.R. Reddy, M. Chen, S. Taduri, S.L. Meyers, M.W. Shankle
Photosynthetica X:X | DOI: 10.32615/ps.2019.137
Ground-level UV-B will stay at a high level in the next several decades and influence sweet potato growth and yield because of the remaining chlorofluorocarbons in the atmosphere. The study explored three UV-B (none, ambient, and elevated/projected) levels on three contrasting sweet potato cultivars (Beauregard, Hatteras, and Louisiana 1188) using sunlit plant growth chambers at Mississippi State University. The results showed that UV-B influenced three cultivars differently. Growth, photosynthetic rate, epidermal and leaf structure of Beauregard were negatively influenced under ambient and elevated UV-B. On the contrary, Hatteras was positively influenced,...
Special issue in honour of Prof. Reto J. Strasser – Photoinhibition of oxygen-evolving complex and photosystem II at chilling stress in the tropical tree species Dalbergia odorifera
Y.-J. YANG, T. LIU, S.-B. ZHANG, W. HUANG
Photosynthetica X:X | DOI: 10.32615/ps.2019.138
Photosystem II is sensitive to chilling stress in tropical tree species. However, the underlying mechanism is not clear. In this study, we examined the effects of chilling and light stress on activities of PSII and oxygen-evolving complex (OEC), in order to resolve the controversy of whether chilling-induced PSII photoinhibition is attributed to the excess-energy model or the two-step scheme. We determined changes in chlorophyll a fluorescence transient and energy distribution in PSII during treatment at chilling-light stress for the tropical tree species Dalbergia odorifera. At chilling temperature, the light-use efficiency was largely...
Photosynthesis, growth, and yield of Paeonia ostii in tree-based agroforestry systems
Y.ZH. YANG, ZH.H. ZHANG, G.X. WANG, Y.Y. YANG, H. GUO
Photosynthetica X:X | DOI: 10.32615/ps.2019.139
Paeonia ostii T. Hong & J.X. Zhang, an emerging oil crop, was intercropped with 3-year-old plantations of pawpaw [Chaenomeles sinensis (Thouin.) Koehne], Chinese toon (Toona sinensis Roem.), and walnut (Juglans regia L.). In order to achieve a better production system, we studied the effects of intercropping on the photosynthesis, growth, and yield of P. ostii. The results showed that different pattern of agroforestry systems changed microclimatic and growing site conditions in comparison to the control treatment. The correlation analysis demonstrated that both similarities and differences were found in relationship...
Photosynthetic activity of common buckwheat (Fagopyrum esculentum Moench) exposed to thermal stress
M. HORNYÁK, A. PŁAŻEK, P. KOPEĆ, M. DZIURKA, J. PASTUSZAK, A. SZCZERBA, T. HURA
Photosynthetica X:X | DOI: 10.32615/ps.2019.140
The aim of the work was to investigate thermal stress effect on photosynthetic activity of common buckwheat. Seedlings of common buckwheat were exposed to 20°C (control) and 30°C (thermal stress). The research involved the Polish cultivar 'Panda' and strain PA15 and determined kinetics of chlorophyll a fluorescence (Chlf), leaf gas exchange, soluble carbohydrate (SC) content in donor leaves, electrolyte leakage as a parameter of cell membrane permeability, and amount of abscisic acid and jasmonates. In 'Panda' and PA15 plants grown at 30°C, most of Chlf parameters improved. 'Panda' plants grown at 30°C demonstrated a higher increase in...
Triadimefon increases drought tolerance through the regulation of photosynthesis and carbohydrate metabolism in rapeseed at bolting stage
F. WANG, F. ZHONG, S. ZHANG, P. ZHANG, F. CHEN, W. LI, S. ZHANG, H. JIANG
Photosynthetica X:X
Drought is a major abiotic factor limiting agricultural crop production. The focus of this study was the effect of triadimefon (TDM) on rapeseed photosynthesis and carbohydrate metabolism in response to drought stress. Results showed that TDM increased plant dry mass per plant and reduced the damage to photosynthetic processes by regulating stomatal and nonstomatal factors, inducing photosynthetic pigment synthesis, and improving photosynthetic activity. Chloroplast degradation and senescence was reduced in rapeseed leaves with TDM under drought stress. TDM restored structural connections between chloroplasts and cell membranes and modified the chloroplasts...
Special issue in honour of Prof. Reto J. Strasser – Photosynthesis response of microalgae (Tetradesmus wisconsinensis) to different inorganic carbon sources probed with chlorophyll fluorescence analysis
E. JANKA, I. UMETANI, M. SPOSOB, R. BAKKE
Photosynthetica X:X | DOI: 10.32615/ps.2019.142
Bicarbonate is an alternative carbon source for production of photosynthetic microalgae, although sparging of CO2-enriched gas is commonly practised, its efficiency seems to be scarcely considered. This study evaluated the photosynthetic activities of microalgae (Tetradesmus wisconsinensis) using chlorophyll fluorescence under different concentrations of bicarbonate (HCO3-) and CO2. Chlorophyll fluorescence analysis showed noticeable difference in photosynthetic activity between the bicarbonate (HCO3-) and CO2 supplied cultures. The JIP-test parameters obtained...
Special issue in honour of Prof. Reto J. Strasser – Chlorophyll fluorescence, leaf gas exchange, and genomic analysis of chromosome segment substitution rice lines exposed to drought stress
K. HUNGSAPRUG, T. KOJONNA, M. SAMLEEPAN, C. PUNCHKHON, W. UT-KHAO, B. KOSITSUP, W. KASETTRANUN, J.L. SIANGLIW, T. TOOJINDA, L. COMAI, K. PLAIMAS, S. CHADCHAWAN
Photosynthetica X:X | DOI: 10.32615/ps.2019.144
This research aims to evaluate the photosynthesis-related parameters in rice chromosome segment substitution lines (CSSL), containing drought-tolerant region from DH212 in a Khao Dawk Mali105 genetic background. Screening at seedling stage indicated that CSSL4 was more tolerant to drought stress than KDML105 with the higher maximal quantum yield of PSII photochemistry. After withholding water, the decline in light-saturated net photosynthetic rate due to drought stress occurred simultaneously with the decrease in electron transport rate and effective quantum yield of PSII photochemistry values, suggesting that stomatal changes affect light-saturated...
Special issue in honour of Prof. Reto J. Strasser – The photosynthetic performance of two Citrus species under long-term aluminum treatment
H. ZHANG, X.Y. LI, L.-S. CHEN, Z.R. HUANG
Photosynthetica X:X | DOI: 10.32615/ps.2019.145
The effect of Al stress on leaf pigment contents, chlorophyll (Chl) a fluorescence transient, and leaf gas exchange of Citrus sinensis and Citrus grandis were investigated in sandy culture with nutrient solution (control) or supplemented by 0.5 mM AlCl3.6H2O (Al toxic) for 54 weeks. We found a significant decline of Chl a, carotenoids (Car), Ch a/b, and Car/Chl (a+b) in C. grandis caused by Al stress. Except the significant increase of Car/Chl (a+b), no remarkable difference was found in C. sinensis. The Al-induced downregulation of CO2...
Special issue in honour of Prof. Reto J. StrasserChlorophyll a fluorescence parameters as indicators of a particular abiotic stress in rice
P. FASEELA, A.K. SINISHA, M. BRESTIČ, J.T. PUTHUR
Photosynthetica X:X | DOI: 10.32615/ps.2019.147
The aim of this study was to evaluate the validity of some chlorophyll (Chl) a fluorescence parameters as early indicators of a particular abiotic stress and also to characterize the effect of different abiotic stresses [high light, NaCl, polyethylene glycol (PEG)-induced osmotic stress, and heavy metals] on the electron transport chain of rice (Oryza sativa L.) seedlings. The results clearly revealed that Chl a fluorescence parameters differ between abiotic stress types and also allowed us to select some parameters which were specifically and intensively affected under different abiotic stresses. We observed that the performance...
Special issue in honour of Prof. Reto J. StrasserA comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass)
B. JIMÉNEZ-FRANCISCO, A. STIRBET, H. CAMPOS, F.V. CONDE-MARTÍNEZ, D. PADILLA-CHACÓN, C. TREJO, C.J. BERNACCHI, G. GOVINDJEE
Photosynthetica X:X | DOI: 10.32615/ps.2019.148
Bouteloua gracilis (blue grama grass) is one of the most drought and grazing tolerant plants in the short-grass ecosystem. To obtain information on their photosystem activities, we measured the fast (< 1 s) chlorophyll a fluorescence transient (the OJIP curve) from their leaves, and isolated cells grown photoautotrophically in suspension in a culture medium, or with added sucrose. One of our goals was to study the effect of different sucrose concentrations (0, 0.15, 0.3, and 3%) on PSII activity in isolated cells. Our results on cells suspended in culture medium, using the JIP-test, showed a decrease in PSII activity at increasing sucrose...
Special issue in honour of Prof. Reto J. Strasser – Can be performance indexes used to select plant growth-promoting rhizobacteria?
A.T. PERBONI, E.G. MARTINAZZO, A.B. MOURA, M.A. BACARIN
Photosynthetica X:X | DOI: 10.32615/ps.2019.149
The plant growth-promoting rhizobacteria (PGPR) are bacteria that colonize the rhizosphere or roots, resulting in a beneficial effect on plants. The evaluation of the PGPR effects is mostly based on growth biometric measurements, but there is little information about using the chlorophyll (Chl) fluorescence. We used rhizobacteria to treat canola seeds and evaluate their effects on leaf area, dry mass, and Chl fluorescence. In order to select PGPR, we suggest the JIP-test as a method to evaluate their effects using the performance indexes. The biometric measurements and JIP-test parameters allowed to group bacterial isolates into different groups. We...
Special issue in honour of Prof. Reto J. StrasserRevisiting JIP-test: An educative review on concepts, assumptions, approximations, definitions and terminology
M. TSIMILLI-MICHAEL
Photosynthetica X:X | DOI: 10.32615/ps.2019.150
The aim of this educative review is, by revisiting the JIP-test as introduced and further elaborated and extended by Professor Reto J. Strasser and his research group, to clarify concepts, assumptions, and approximations on which it is based, as well as definitions and terms it uses, reminding that it is meant to evaluate impacts of environmental stresses -factors and/or perturbations - on the photosynthetic structure and function. It analyses how the JIP-test, based on the Theory of Energy Fluxes and adopting Duysens' concept, translates the OJIP polyphasic chlorophyll a fluorescence rise kinetics, emitted by PSII, into biophysical parameters...
N-glycosylation regulates photosynthetic efficiency of Arabidopsis thaliana
Q.-S. JIAO, G.-T. NIU, F.-F. WANG, J.-Y. DONG, T.-S. CHEN, C.-F. ZHOU, Z. HONG
Photosynthetica X:X | DOI: 10.32615/ps.2019.153
N-glycosylation is one of the most important protein modifications in eukaryotes. It has been well established that N-glycosylation plays multiple roles in regulating stress tolerance of plants. However, the effects and mechanism of N-glycosylation on photosynthesis have not been well understood. In the present study, an obvious decrease in photosynthetic capacity and dry mass were detected in alg3-3 and cgl1-1, two typical mutants in N-glycosylation process. The maximal photochemical efficiency of PSII decreased significantly in cgl1-1. The values of effective quantum yield of PSII photochemistry, rate of photosynthetic electron...
Toxic effects of potassium permanganate on photosystem II activity of cyanobacteria Microcystis aeruginosa
L. LI, X.L. PAN, G.J. MU
Photosynthetica X:X | DOI: 10.32615/ps.2019.155
Effects of potassium permanganate (KMnO4) on PSII of Mycrocystis aeruginosa were investigated by measuring the chlorophyll fluorescence in vivo. KMnO4 exposure reduced the rate of oxygen evolution and cell growth. High concentration of KMnO4 (10 mg L-1) decreased the fast phase and increased the slow phase of QA- reoxidation kinetics. Electron transport after QA was blocked, resulting in a considerable amount of QA- reoxidation being performed via S2(QAQB)- charge recombination. KMnO4...
Different responses of photosystem and antioxidant defense system to three environmental stresses in wheat seedlings
N. WU, H.T. MAO, M.Y. CHEN, J. DONG, M. YUAN, Z.W. ZHANG, S. YUAN, H.Y. ZHANG, Y.E. CHEN
Photosynthetica X:X | DOI: 10.32615/ps.2019.156
To investigate the adaptive mechanism of wheat under high light, osmotic stress, and salt stress, redox regulation, and photosynthesis were compared. Under high light, the activities of antioxidant enzymes, except for catalase, significantly increased and then decreased after 1 and 3 h, respectively. Under osmotic stress, antioxidant enzyme activities significantly increased and subsequently declined. Except for superoxide dismutase, salt stress induced a significant increase in all the antioxidant enzyme activities. A significant decrease of D1 protein by high light and osmotic stress, strong photophosphorylation of D1 and D2 under high light and...
Nitric oxide participates in the regulation of ascorbate-glutathione cycle and water physiological characteristics of Arabidopsis thaliana by NaHS
C.J. SHAN, Y.Y. JIN, Y. ZHOU, H. LI
Photosynthetica X:X | DOI: 10.32615/ps.2019.158
We investigated the role of nitric oxide (NO) in the regulation of ascorbate-glutathione (AsA-GSH) cycle and water physiological characteristics of Arabidopsis thaliana by sodium hydrosulfide (NaHS). NaHS markedly increased the contents of H2S, NO, chlorophyll (Chl), and carotenoids, the activity of AsA-GSH cycle, ascorbate/dehydroascorbate ratio, net photosynthetic rate, Chl fluorescence parameters, transpiration rate, stomatal conductance, and relative water content in leaves and the biomass of wild-type Arabidopsis. However, NaHS markedly decreased malondialdehyde content and electrolytic leakage. Except H2S,...
Exogenous spermidine enhances salt-stressed rice photosynthetic performance by stabilizing structure and function of chloroplastand thylakoid membranes
D.X. JIANG, X. CHU, M. LI, J.J. HOU, X. TONG, Z.P. GAO, G.X. CHEN
Photosynthetica X:X | DOI: 10.32615/ps.2019.160
Spermidine (Spd) is a ubiquitous low-molecular-mass aliphatic amine that acts in abiotic stress tolerance in plants. We investigated how the treatment with exogenous Spd contributed to the protection against salt stress in rice chloroplasts. Analysis of the chlorophyll (Chl) fluorescence showed that there were many negative effects of salinity on different sites of the photosynthetic machinery; however, these were alleviated by adding Spd. Spd prevented the damage of structure and function of chloroplasts under salt stress; thylakoid membrane protein components and photosynthetic pigments were not severely affected by salinity in Spd-treated plants....
Special issue in honour of Prof. Reto J. StrasserCharacterization of photosynthetic performance during natural leaf senescence in winter wheat: Multivariate analysis as a tool for phenotypic characterization
M. VILJEVAC VULETIĆ, V. ŠPANIĆ
Photosynthetica X:X | DOI: 10.32615/ps.2019.162
In the current research, we used chlorophyll fluorescence measurements and photosynthetic pigment content to evaluate onset and rate of the flag leaf senescence every 7 d beginning at the flowering stage (0 d after flowering, DAF) until late senescence stage (35 DAF) on three winter wheat field-grown varieties (Alka, Žitarka, and Olimpija) with the similar maturation time. OJIP curves implied that flag leaves of variety Alka detained the longest photosynthetic efficiency, while the earliest symptoms of senescence onset were indicated by positive L-band at 7 DAF and K-band at 14 DAF in variety Olimpija. A shift in I-P phase in the wheat flag leaves...