Photosynthetica, 2011 (vol. 49), issue 2

Photosynthetica 2011, 49(2):227-238 | DOI: 10.1007/s11099-011-0024-0

Effects of the interaction between ozone and carbon dioxide on gas exchange, photosystem II and antioxidants in rice leaves

H. Kobayakawa1, K. Imai1,*
1 School of Agriculture, Meiji University, Kawasaki, Kanagawa, Japan

To understand the interactive effects of O3 and CO2 on rice leaves; gas exchange, chlorophyll (Chl) fluorescence, ascorbic acid and glutathione were examined under acute (5 h), combined exposures of O3 (0, 0.1, or 0.3 cm3 m-3, expressed as O0, O0.1, or O0.3, respectively), and CO2 (400 or 800 cm3 m-3, expressed as C400 or C800, respectively) in natural-light gas-exposure chambers. The net photosynthetic rate (P N), maximum (Fv/Fm) and operating (Fq'/Fm') quantum efficiencies of photosystem II (PSII) in young (8th) leaves decreased during O3 exposure. However, these were ameliorated by C800 and fully recovered within 3 d in clean air (O0 + C400) except for the O0.3 + C400 plants. The maximum PSII efficiency at 1,500 μmol m-2 s-1 PPFD (Fv'/Fm') for the O0.3 + C400 plants decreased for all measurement times, likely because leaves with severely inhibited P N also had a severely damaged PSII. The P N of the flag (16th) leaves at heading decreased under O3 exposure, but the decline was smaller and the recovery was faster than that of the 8th leaves. The Fq'/Fm' of the flag leaves in the O0.3 + C400 and O0.3 + C800 plants decreased just after gas exposure, but the Fv/Fm was not affected. These effects indicate that elevated CO2 interactively ameliorated the inhibition of photosynthesis induced by O3 exposure. However, changes in antioxidant levels did not explain the above interaction.

Keywords: ascorbic acid; chlorophyll fluorescence; elevated CO2; glutathione; net photosynthesis; Oryza sativa; ozone; quantum efficiency; respiration; stomatal conductance

Received: July 16, 2010; Accepted: March 23, 2011; Published: June 1, 2011Show citation

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Kobayakawa, H., & Imai, K. (2011). Effects of the interaction between ozone and carbon dioxide on gas exchange, photosystem II and antioxidants in rice leaves. Photosynthetica, 49(2), 227-238. doi: 10.1007/s11099-011-0024-0.
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    References

    1. Baier, M., Kandlbinder, A., Golldack, D., Dietz, K.-J.: Oxidative stress and ozone: perception, signaling and response. - Plant Cell Environ. 28: 1012-1020, 2005. Go to original source...
    2. Baker, N.R.: Chlorophyll fluorescence: A probe of photosynthesis in vivo. - Annu. Rev. Plant Biol. 59: 89-113, 2008. Go to original source...
    3. Booker, F.L., Fiscus, E.L.: The role of ozone flux and antioxidants in the suppression of ozone injury by elevated CO2 in soybean. - J. Exp. Bot. 56: 2139-2151, 2005. Go to original source...
    4. Cabrera, H., Dawson, S.V., Stromberg, C.: A California air standard to protect vegetation from ozone. - Environ. Pollut. 53: 397-408, 1988. Go to original source...
    5. Donnelly, A., Jones, M.B., Burke, J.I., Schnieders, B.: Elevated CO2 provides protection from O3 induced photosynthetic damage and chlorophyll loss in flag leaves of spring wheat (Triticum aestivum L., cv. 'Minaret). - Agric. Ecosyst. Environ. 80: 159-168, 2000. Go to original source...
    6. Environ. Improve. Div., Tokyo Metropolitan Government Bureau Environ.: [Review report on the photochemical oxidant measures.] - J. Jap. Soc. Atmos. Environ. 40: A65-A77, 2005. [In Jap.]
    7. Haun, J.R.: Visual quantification of wheat development. - Agron. J. 65: 116-119, 1973. Go to original source...
    8. Heath, R.L.: Possible mechanisms for the inhibition of photosynthesis by ozone. - Photosynth. Res. 39: 439-451, 1994. Go to original source...
    9. Hoshikawa: The Growing Rice Plant. - Nobunkyo, Tokyo 1989. [In Jap.]
    10. Imai, K., Kobori, K.: Effects of the interaction between ozone and carbon dioxide on gas exchange, ascorbic acid content, and visible leaf symptoms in rice leaves. - Photosynthetica 46: 387-394, 2008. Go to original source...
    11. Inada, H., Yamaguchi, M., Satoh, R., Hoshino, D., Nagasawa, A., Negishi, Y., Nouchi, I., Kobayashi, K., Izuta, T.: Effects of ozone on photosynthetic components and radical scavenging system in leaves of rice (Oryza sativa L.). - J. Agric. Meteorol. 64: 243-255, 2008. Go to original source...
    12. Ishioh, T., Imai, K.: [Effects of atmospheric ozone and carbon dioxide concentrations on gas exchanges, contents of Rubisco and chlorophyll in leaves of lowland rice.] - Proc. Kanto Branch,Crop Sci. Soc. Jap. 20: 54-55, 2005. [In Jap.]
    13. Ishioh, T., Kobori, K., Imai, K.: The detrimental effect of tropospheric O3 on lowland rice is meliorated by elevated CO2. - In: Toriyama, K., Heong, K. L., Hardy, B.,(ed.): Rice is Life: Scientific Perspectives for the 21st Century. Pp 557-560. IRRI, Los Baños 2005.
    14. Ito, A., Udagawa, T., Uchijima, Z.: [Phytometrical studies of crop canopies. II. Canopy structure of rice crops in relation to varieties and growing stage.] - Jap. J. Crop. Sci. 42: 334-342, 1973. [In Jap.] Go to original source...
    15. Kobayashi, K.: [Assessing the impacts of tropospheric ozone on agricultural production.] - J. Jap. Soc. Atmos. Environ. 34: 162-175, 1999. [In Jap.]
    16. Mano, J., Khorobrykh, S., Amako, K.: [Reactive oxygen species and antioxidants associated with photosynthesis.] - Low Temp. Sci. 67: 179-195, 2009. [In Jap.]
    17. McKee, I.F., Eiblmeier, M., Polle, A.: Enhanced ozonetolerance in wheat grown at an elevated CO2 concentration: ozone exclusion and detoxification. - New Phytol. 137: 275-284, 1997. Go to original source...
    18. Morita, S., Tanaka, K.: Detoxification of active oxygen species and tolerance in plants exposed to air pollutants and CO2. - In: Omasa, K., Saji, H., Youssefian, S., Kondo, N. (ed.): Air Pollution and Plant Biotechnology. Prospects for Phytomonitoring and Phytoremediation. Pp. 253-267. Springer-Verlag, Tokyo 2002. Go to original source...
    19. Mulholland, B.J., Craigon, J., Black, C.R., Colls, J.J., Atherton, J., Landon, G.: Impact of elevated atmospheric CO2 and O3 on gas exchange and chlorophyll content in spring wheat (Triticum aestivum L). - J. Exp. Bot. 48: 1853-1863, 1997.
    20. Nakanishi, J., Shinozaki, H., Inoue, K.: [Ozone - Photochemical Oxidant.] - Maruzen Co., Tokyo 2009. [In Jap.]
    21. Nouchi, I.: Changes in antioxidant levels and activities of related enzymes in rice leaves exposed to ozone. - Soil. Sci. Plant Nutr. 39: 309-320, 1993. Go to original source...
    22. Nouchi, I., Ito, O., Harazono, Y., Kouchi, H.: Acceleration of 13C-labelled photosynthate partitioning from leaves to panicles in rice plants exposed to chronic ozone at the reproductive stage. - Environ. Pollut. 88: 253-260, 1995. Go to original source...
    23. Olszyk, D.M., Wise, C.: Interactive effects of elevated CO2 and O3 on rice and flacca tomato. - Agr. Ecosyst. Environ. 66: 1-10, 1997. Go to original source...
    24. Overmyer, K., Tuominen, H., Kettunen, R., Betz, C., Langebartels, C., Sandermann, H., Jr, Kangasjärvi, J.: Ozonesensitive Arabidopsis rcd1 mutant reveals opposite roles for ethylene and jasmonate signaling pathways in regulating superoxide-dependent cell death. - Plant Cell 12: 1849-1862, 2000.
    25. Pang, J., Kobayashi, K., Zhu, J.G.: Yield and photosynthetic characteristics of flag leaves in Chinese rice (Oryza sativa L.) varieties subjected to free-air release of ozone. - Agr. Ecosyst. Environ. 132: 203-211, 2009. Go to original source...
    26. Papageorgiou, G.C., Govindjee. (ed.): Chlorophyll a Fluorescence. A Signature of Photosynthesis. Advances in Photosynthesis and Respiration. Vol. 19. - Springer, Dordrecht 2004. Go to original source...
    27. Rai, R., Agrawal, M.: Evaluation of physiological and biochemical responses of two rice (Oryza sativa L.) cultivars to ambient air pollution using open top chambers at a rural site in India. - Sci. Total Environ. 407: 679-691, 2008. Go to original source...
    28. Rai, R., Agrawal, M., Agrawal, S.B.: Threat to food security under current levels of ground level ozone: A case study for Indian cultivars of rice. - Atmos. Environ. 44: 4272-4282, 2010. Go to original source...
    29. Rao, M.V., Hale, B.A., Ormrod, D.P.: Amelioration of ozoneinduced oxidative damage in wheat plants grown under high carbon dioxide (Role of antioxidant enzymes). - Plant Physiol. 109: 421-432, 1995. Go to original source...
    30. Reid, C.D., Fiscus, E.L.: Ozone and density affect the response of biomass and seed yield to elevated CO2 in rice. - Global Change Biol. 14: 60-76, 2008.
    31. Roe, J.H., Oesterling, M.J.: The determination of dehydroascorbic acid and ascorbic acid in plant tissues by the 2,4-dinitrophenylhydrazine method. - J. Biol. Chem. 152: 511-517, 1944.
    32. Sasaki-Sekimoto, Y., Taki, N., Obayashi, T., Aono, M., Matsumoto, F., Sakurai, N., Suzuki, H., Hirai, M. Y., Noji, M., Saito, K., Masuda, T., Takamiya, K.-I., Shibata, D., Ohta, H.: Coordinated activation of metabolic pathways for antioxidants and defence compounds by jasmonates and their roles in stress tolerance in Arabidopsis. - Plant J. 44: 653-668, 2005. Go to original source...
    33. Sonoike, K.: [Basics of the measurements of photosynthesis by pulse amplitude modulation.] - Low Temp. Sci. 67: 507-524, 2009. [In Jap.]
    34. Torsethaugen, G., Pell, E.J., Assman, S.M.: Ozone inhibits guard cell K+ channels implicated in stomatal opening. - Proc. nat. Acad. Sci. USA 96: 13577-13582, 1999. Go to original source...
    35. Toyama, S., Yoshida, M., Niki, T., Ohashi, T., Koyama, I.: Studies on ultrastructure and function of photosynthetic apparatus in rice cells. IV. Effects of low dose and intermittent fumigation of ozone on the ultrastructure of chloroplasts in rice leaf cells. - Jap. J. Crop Sci. 58: 664-672, 1989. Go to original source...
    36. WMO WDCGG: Data Summary No. 34. Pp. 1-13. JMA (Japan), Tokyo 2010.
    37. Yamaguchi, M., Inada, H., Satoh, R., Hoshino, D., Nagasawa, A., Negishi, Y., Sasaki, H., Nouchi, I., Kobayashi, K., Izuta, T.: Effects of ozone on the growth, yield and leaf gas exchange rates of two Japanese cultivars of rice (Oryza sativa L.) - J. Agr. Meteorol. 64: 131-141, 2008. Go to original source...

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