Photosynthetica, 2011 (vol. 49), issue 4

Photosynthetica 2011, 49(4):564-572 | DOI: 10.1007/s11099-011-0068-1

Drought responses in Aleppo pine seedlings from two wild provenances with different climatic features

M. Michelozzi1,*, F. Loreto2, R. Colom1, F. Rossi1, R. Calamassi3
1 Istituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche, Area di Ricerca di Firenze, Sesto Fiorentino (Firenze), Italy
2 Istituto per la Protezione delle Piante, Consiglio Nazionale delle Ricerche, Area di Ricerca di Firenze, Sesto Fiorentino (Firenze), Italy
3 Dipartimento di Biotecnologie Agrarie, Università degli Studi di Firenze, Firenze, Italy

Global warming will likely exacerbate the negative effects of limited water availability in the Mediterranean area. The Italian Aleppo pine (Pinus halepensis Mill.) provenances are distributed along the coasts except Otricoli provenance growing in an unusual location between 300 and 1,000 m a. s. l., in Umbria (central Italy). The aim of the present study was to investigate the photosynthetic response to a 28-day-long drought and to a subsequent reestablishment of water availability in Otricoli and North Euboea (Greece) provenances, representing different locations along a rainfall gradient in the natural range of this species. Six-month-old seedlings were used in this experiment since at this age Aleppo pine plants in Mediterranean climate face their first water stress potentially affecting plant survival. Water potential (ψw), net photosynthesis and stomatal conductance decreased during drought in both provenances and showed minimal values 28 days after beginning the treatment (DAT). Otricoli seedlings adjusted ψw gradually as the stress level increased and 21 DAT showed a lower ψw than North Euboea. In contrast, in North Euboea seedlings ψw that was not affected until 21 DAT rapidly dropped to a minimum of -3.81 MPa 28 DAT. At the onset of the stress the intercellular CO2 concentration (C i) was reduced, and the "instrinsic" water-use efficiency (WUEi) was enhanced in both provenances, as stomatal conductance decreased more rapidly than photosynthesis. However, 28 DAT, C i increased and WUEi decreased as stomatal conductance and photosynthesis declined to minimum levels, revealing nonstomatal limitations of photosynthesis. A rapid decrease in PSII maximal photochemical efficiency estimated by chlorophyll (Chl) fluorescence (Fv/Fm) was also observed when the stress became severe. At the final stage of water stress, North Euboea seedlings maintained significantly higher values of Fv/Fm than Otricoli seedlings. Upon rewatering, photosynthesis did not fully recover in Otricoli seedlings (41 DAT), while all other parameters recovered to control levels in both provenances. No drought-induced physiological differences were consistent with the regional climatic features of these two provenances. Our results suggest that phenotypic plasticity in drought response may help Otricoli provenance cope with global warming, but that recurrent drought episode may slow down the primary productivity of this provenance.

Received: January 28, 2011; Accepted: September 10, 2011; Published: December 1, 2011Show citation

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Michelozzi, M., Loreto, F., Colom, R., Rossi, F., & Calamassi, R. (2011). Drought responses in Aleppo pine seedlings from two wild provenances with different climatic features. Photosynthetica, 49(4), 564-572. doi: 10.1007/s11099-011-0068-1.
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    References

    1. Baker, N.R., Bowyer, J.R. (ed.): Photoinhibition of Photosynthesis from Molecular Mechanisms to the Field. - BIOS Scientific Publishers, Oxford 1994.
    2. Baroli, I., Melis, A.: Photoinhibitory damage is modulated by rate of photosynthesis and by photosystem II light-harvesting chlorophyll antenna size. - Planta 205: 288-296, 1998. Go to original source...
    3. Boddi, S., Morassi Bonzi, L., Calamassi, R.: Structure and ultrastructure of Pinus halepensis primary needles. - Flora 197: 10-23, 2002. Go to original source...
    4. Borghetti, M., Cinnirella, S., Magnani, F., Saracino, A.: Impact of long-term drought on xylem embolism and growth in Pinus halepensis Mill. - Trees 12: 187-195, 1998. Go to original source...
    5. Brodribb, T.: Dynamics of changing intercellular CO2, concentration (ci) during drought and determination of minimum functional ci. - Plant Physiol. 111: 179-185, 1996. Go to original source...
    6. Björkman O., Demmig, B.: Photon yields of O2 evolution and chlorophyll fluorescence characteristic at 77 K among vascular plants of diverse origin. - Planta 170: 489-504, 1987. Go to original source...
    7. Bukhov, N., Carpentier, R.: Alternative photosystem I-driven electron transport routes: mechanisms and functions. - Photosynth. Res. 82: 17-33, 2004. Go to original source...
    8. Calamassi, R.: [Identification of provenances of Pinus halepensis Mill on the basis of the anatomical and morphological needle structures.] - Ann. Sci. For. 43: 281-298, 1986. [In French.] Go to original source...
    9. Calamassi, R., Falusi, M., Tocci, A.: [Geographical variation and resistance to water stress in seeds of Pinus halepensis Mill., Pinus brutia Ten. and Pinus eldarica Medw. - Ann. Ist. Sper. Selv. Arezzo XI: 195-228, 1980. [In Italian.]
    10. Calamassi, R., Falusi, M., Tocci, A.: [Effects of temperature and stratification on seed germination of Pinus halepensis Mill.] - Silvae Gen. 33: 133-139, 1984. [In French.]
    11. Calamassi, R., Falusi, M., Principe, M.: Relations between apical structure and growth patterns in Pinus halepensis Mill seedlings. - Giorn. Bot. It. 122: 321-338, 1988. Go to original source...
    12. Calamassi, R., Della Rocca, G., Falusi, M., Paoletti, E., Strati, S.: Resistance to water stress in seedlings of eight European provenances of Pinus halepensis Mill. - Ann. Forest. Sci. 58: 663-672, 2001.
    13. Chaves, M. M.: Effects of water deficit on carbon assimilation. - J. Exp. Bot. 42: 1-16, 1991. Go to original source...
    14. Chaves, M.M, Pereira, J.S., Maroco, J., Rodrigues, M.L., Ricardo, C.P.P., Osório, M.L.: How plants cope with water stress in the field: photosynthesis and growth. - Ann. Bot. 89: 907-916, 2002. Go to original source...
    15. Chaves, M.M., Maroco, J.P., Pereira, J.S.: Understanding plant responses to drought from genes to the whole plant. - Funct. Plant Biol. 30: 239-264, 2003. Go to original source...
    16. Craig, D.A., Macalady, A.K., Chenchouni H., et al.: A global overview of drought and heat-induced tree mortality reveals emerging climate change risks for forests. - Forest Ecol. Manag. 259: 660-684, 2010.
    17. Eccher, A.: [Observations on Italian provenances of Aleppo pine.] - In: Proc. 6th World Forest Congress 2: 1644-1647, 1996. [In French.]
    18. Ellsworth, D.S., Reich, P. B.: Water relations and gas exchange of Acer saccharum seedlings in contrasting natural light and water regimes. - Tree Physiol. 10: 1-20, 1992. Go to original source...
    19. Evans, J. R., Loreto, F.: Acquisition and diffusion of CO2 in higher plant leaves. - In: Leegood, R. C., Sharkey, T. D., von Caemmerer, S. (ed.): Photosynthesis: Physiology and Metabolism. Pp. 321-351. Kluwer Acad. Publisher, Dordrecht 2000. Go to original source...
    20. Falusi, M., Calamassi R., Tocci A.: Sensitivity of seed germination and seedling root growth to moisture stress in four provenances of Pinus halepensis Mill. - Silvae Genet. 3: 4-9, 1983.
    21. Falusi, M., Calamassi, R., Tocci, A.: [Frost resistance in Pinus halepensis Mill., Pinus brutia Ten. and Pinus eldarica Medw.] - Atti Soc. Tosc. Sci. Nat. - Mem., serie B: 91, 1984. [In Italian.]
    22. FAO Projet 4bis: [International experiences on Pinus halepensis and Pinus brutia.] - Rome, 1974. [In French.]
    23. Flexas, J., Bota, J., Loreto, F., Cornic, G., Sharkey, T.D.: Diffusive and metabolic limitations to photosynthesis under drought and salinity in C3 plants. - Plant Biol. 6: 269-279, 2004. Go to original source...
    24. Gamon, J.A., Pearcy, R.W.: Photoinhibition in Vitis californica: interactive effects of sunlight, temperature and water status. - Plant Cell Environ. 13: 191-200, 1990. Go to original source...
    25. Genty, B., Briantais, J.-M., Vieira Da Silva, J.B.: Effects of drought on primary photosynthetic processes of cotton leaves. - Plant Physiol. 83: 360-364, 1987. Go to original source...
    26. Genty, B., Briantais, J.-M, Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. Biophys. Acta 990: 87-92, 1989. Go to original source...
    27. Gimenez, C., Mitchell, V.J., Lawlor, D.W.: Regulation of photosynthetic rate of two sunflower hybrids under water stress. - Plant Physiol. 96: 635-643. 1992. Go to original source...
    28. Havaux, M., Canaani, O., Malkin, S.: Inhibition of photosynthetic activities under slow water stress measured in vivo by the photoacoustic method. - Physiol. Plant. 70: 503-510, 1987. Go to original source...
    29. Johnson, R.C., Mornhinweg, D.W., Ferris, D.M., Heitholt, J.J.: Leaf photosynthesis and conductance of selected Triticum species at different water potentials. - Plant Physiol. 83: 1014-1017, 1987. Go to original source...
    30. Lawlor, D.W.: Water stress induced changes in photosynthesis, photorespiration and CO2 compensation concentration of wheat. - Photosynthetica 10: 378-387, 1976.
    31. Lawlor, D.W., Cornic, G.: Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. - Plant Cell Environ. 25: 275-294, 2002. Go to original source...
    32. Maxwell, D.P., Falk, S., Huner, N.P.A.: Photosythem II excitation pressure and development of resistance to photoinhibition I. Light harvesting complex II abundance and zeaxanthin content in Chlorella vulgaris. - Plant Physiol. 107: 687-694, 1995. Go to original source...
    33. Maxwell, K., Johnson, G. N.: Chlorophyll fluorescence - a practical guide. - J. Exp. Bot. 51: 659-668, 2000. Go to original source...
    34. Meyer, S., De Kouchkovsky, Y.,: Electron transport, photosystem II reaction centres and chlorophyll-protein complexes of thylakoids of drought resistant and sensible lupin plants. - Photosynth. Res. 32: 95-107, 1993. Go to original source...
    35. Nahal, I.: The Mediterranean climate from a biological viewpoint. - In: Di Castri, F. (ed.), Ecosystems of the World. Pp. 63-86. Elsevier, Amsterdam 1981.
    36. Ni, B.R., Pallardy, S.G.: Response of gas exchange to water stress in woody angiosperm seedlings: net photosynthesis, leaf conductance, and water use efficiency. - Tree Physiol 8: 1-9, 1991. Go to original source...
    37. Ort, D.R, Oxborough, K., Wise, R.R.: Depression of photosynthesis in attached leaves of Helianthus annuus at low water potentials. - Plant Physiol. 96: 1018-1025, 1994.
    38. Parker, W.C., Pallardy, S.G.: Gas exchange during a soil drying cycle in seedlings of four black walnut (Juglans nigra L.) families. - Tree Physiol. 9: 339-348, 1991. Go to original source...
    39. Pockman, W.T., Sperry, J.S.: Vulnerability to xylem cavitation and the distribution of Sonoran desert vegetation. - Amer. J. Bot. 87: 1287-1299, 2000. Go to original source...
    40. Powles, S.: Photoinhibition of photosynthesis induced by visible light. - Ann. Rev. Plant Physiol. 35: 15-44, 1984. Go to original source...
    41. Prieto, P., Peñuelas, J., Lloret, F., Llorens, L., Estiarte, M.: Experimental drought and warming decrease diversity and slow down post-fire succession in a Mediterranean shrubland. - Ecography 32: 623-636, 2009. Go to original source...
    42. Quick, W.P., Chaves, M.M., Wendler, R.: The effect of water stress on photosynthetic carbon metabolism in four species grown under field conditions. - Plant Cell. Environ. 15: 25-35, 1992. Go to original source...
    43. Regato., P.: Adapting to Global Change: Mediterranean Forests. - IUCN Centre for Mediterranean Cooperation, Malaga 2008.
    44. Schiller, G.: Eco-physiology of Pinus halepensis Mill. and Pinus brutia Ten. - In: Ne'eman, G., Trabaud, L. (ed.): Ecology, Biogeography and Management of Mediterranean Pine Forest Ecosystems (Pinus halepensis and Pinus brutia). Pp. 51-65. Backhuys Publishers-Wil, R. Peters, Amsterdam 2000.
    45. Schiller, G., Atzmon, N.: Performance of Aleppo pine (Pinus halepensis) provenances grown at the edge of the Negev desert: a review. - J. Arid Environ. 73: 1051-1057, 2009. Go to original source...
    46. Schiller, G., Brunori, A.: Aleppo pine (Pinus halepensis Mill.) in Umbria (Italy) and its relation to native Israeli populations". - Israel J. Bot. 41: 123-127, 1992.
    47. Schiller, G., Conkle, M.T., Grunwald, C.: Local differentiation among Mediterranean populations of Aleppo pine in their isoenzymes. - Silva Genet. 35: 11-19, 1986.
    48. Schreiber, U., Bilger, W., Neubauer, C.: Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. - In: Schulze, E. D., Martyn, M., Caldwell, M. M. (ed.): Ecophysiology of Photosynthesis. Pp. 49-70. Springer-Verlag, Berlin 1994. Go to original source...
    49. Semerci, A., Cobb, N.: A global overview of drought and heatinduced tree mortality reveals emerging climate change risks for forests. - Forest Ecol.Manag. 259: 660-684, 2010.
    50. Sharkey, T.D., Seemann, J.R.: Mild water stress effects on carbon-reduction-cycle intermediates, ribulose bisphosphate carboxylase activity, and spatial homogeneity of photosynthesis in intact leaves. - Plant Physiol. 89: 1060-1065, 1989. Go to original source...
    51. Sperry, J.S., Donnelly J.R., Tyree M.T.: A method for measuring hydraulic conductivity and embolism in xylem. - Plant Cell Environ. 11: 35-40, 1988. Go to original source...
    52. Sperry, J.S.: Hydraulic constraints on plant gas exchange. - Agric. For. Meteorol. 104:13-23, 2000. Go to original source...
    53. Sultan, S.E.: Phenotypic plasticity for plant development, function, and life-history. - Trends Plants Sci. 5: 537-542, 2000. Go to original source...
    54. Terashima, I.: Anatomy of non-uniform leaf photosynthesis. - Photosynth. Res. 31: 195-212, 1992. Go to original source...
    55. Tezara, W., Mitchell, V.J., Driscoll, S.D., Lawlor, D.W.: Water stress inhibits plant photosynthesis by decreasing coupling factor and ATP. - Nature 401: 914-917, 1999. Go to original source...
    56. Tognetti, R., Michelozzi, M., Giovanelli, A.: Geographical variation in water relations, hydraulic architecture and terpene composition of Aleppo pine seedlings from Italian provenances. - Tree Physiol. 17: 241-250, 1997. Go to original source...
    57. Root, T.L., Price, J.T., Hall, K.R., Schneider, S.H., Rosenzweig, C., Pounds, J. A.: Fingerprints of global warming on wild animals and plants. - Nature 421: 57-60, 2003. Go to original source...
    58. Umezawa, T., Nakashima, K., Miyakawa, T., Kuromori, T., Tanokura, M., Shinozaki, K., Yamaguchi-Shinozaki, K.: Molecular Basis of the Core Regulatory Network in ABA Responses: Sensing, Signaling and Transport. - Plant Cell Physiol. 51: 1821-1839, 2010.
    59. UNESCO-FAO: [Carte bioclimatique de la zone mediterranéenne. Notice explicative. Recherches sur le zone aride.] - XXI. UNESCO Paris et FAO Rome 1963.
    60. von Caemmerer, S., Farquar, G.D.: Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. - Planta 153: 376-387, 1981. Go to original source...

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