14.1. 2020, Laboratory of Translational Metabolism

Laboratory of Translational Metabolism

PhD project: Influence of proteins in the protection against tooth caries

About 90% worldwide adults suffer from dental caries on enamel and root surfaces. The resistance of the lucky rest of population (10%) against the development of dental caries still remains unclear.

Dental caries is a multifactorial disease that includes the participation of cariogenic bacteria, salivary components (proteins, peptides), carbohydrates, etc. The anti-caries resistance could be caused by the presence of specific proteins (e.g. antibacterial agents) inside the tooth tissues (pulp, dentin or enamel) or inside the oral cavity (saliva, thin pellicle on the tooth surface).

The PhD project aims to find these “anti-caries” proteins (or peptides), which could help to protect population against tooth caries.

Candidate’s profile (requirements):

We are seeking self-motivated candidates with master's degree or equivalent in analytical chemistry, biochemistry, physiology, medicine or related fields, or those expecting to obtain their degree this year. Candidates should be fluent in English. Experiences with protein separation methods and techniques (electrophoresis, HPLC, mass spectrometry) are advantages.

Relevant publications:

Jágr M, Eckhardt, et al., Proteomic analysis of human tooth pulp proteomes – Comparison of caries-resistant and caries-susceptible persons, J Prot., 2016; 145: 127-136.

Eckhardt A, et al., Proteomic analysis of human tooth pulp: proteomics of human teeth, JOE, 2014; 8:1961-6.

Cooperation: The Department of Oral and Maxillofacial Surgery of School of Medical Dentistry, 1st Medical Faculty of Charles University, Prague (Prof. R. Foltán)

Supervisor: Adam Eckhardt, PhD