Modified nucleoside triphosphates with unnatural nucleobases for enzymatic synthesis of modified DNA
Bioorganic and Medicinal Chemistry of Nucleic Acids
Abstract
The project will deal with the study and development of the chemical synthesis of diverse modified nucleoside triphosphates derived from d5SICS and dMMO2 nucleosides, which are used in the extension of the genetic alphabet. New derivatives bearing useful functional groups will be designed and synthesized aiming at the modification of DNA in the major groove. Substrate activity of these dNTPs with diverse DNA polymerases will be studied and they will be used for site-specific modification of DNA or in enzymatic synthesis of hypermodified DNA.
Field of study: Organic chemistry
Reference: Seo, Y. J.; Malyshev, D. A.; Lavergne, T.; Ordoukhanian, P.; Romesberg, F. E. Site-specific Labeling of DNA and RNA Using an Efficiently Replicated and Transcribed Class of Unnatural Base Pairs. J. Am. Chem. Soc. 2011, 133, 19878–19888.
Universities
PhD students must be enrolled in a partner university and will be employed by the IOCB Prague at the same time (part-time or full time), which results in a competitive salary (a scholarship from the university + a salary from the IOCB). Each university has its own process, terms, and deadlines for PhD applications, which is separate from the IOCB recruitment process. You may discuss the details with the respective PI.
How to apply
Please return to the PhD projects at IOCB Prague – Call for Applications 2020 page and follow the instructions.