Biologia plantarum - Latest articles
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Molecular characterization of the promoter of the stress-inducible ZmMYB30 gene in maizeOriginal Papers
J. LUO, C.M. YU, M. YAN, Y. H. CHEN
Biologia plantarum 64: 200-210, 2020 | DOI: 10.32615/bp.2020.011
The ZmMYB30 is a member of the myeloblastosis (MYB) transcription factor superfamily, which has been shown to be a transcription regulator in abiotic stress tolerance in maize (Zea mays). To further identify the biological function of ZmMYB30 and reveal how its expression is induced in response to stress, we isolated the ZmMYB30 promoter and conducted a functional analysis. A 1461-bp promoter fragment was cloned and sequenced. Motif prediction using PlantCARE revealed several stress-responsive elements in the promoter sequence. Deletions in the promoter sequence affected the activity of the promoter and demonstrated that...
Characterization and functional analysis of microRNA399 in Cunninghamia lanceolataOriginal Papers
F.R. ZHU, Z.B. QIU, Y.M. ZHANG, X. R. ZHANG, W. L.WANG
Biologia plantarum 64: 193-199, 2020
The miR399 is a conserved microRNA (miRNA) family, and it has been characterized as an essential regulator of phosphorus transport in plants. However, the biological function of miR399 in Cunninghamia lanceolata is still largely unclear. In this study, the comparison of mature miR399 sequence revealed a high similarity between Arabidopsis thaliana and C. lanceolate, and the pre-miR399 was capable of forming a typical stem-loop hairpin structure. A gene PHOSPHATE 2 (PHO2) was identified as a target of cln-miR399 using 5' rapid amplification of cDNA ends. Furthermore, the relationship between cln-miR399...
Differences in physiological traits at the initial stage of Fusarium head blight infection in wheatOriginal Papers
V. SPANIC, Z. ZDUNIC, G. DREZNER, M. VILJEVAC VULETIC
Biologia plantarum 64: 185-192, 2020 | DOI: 10.32615/bp.2020.014
Wheat (Triticum aestivum L.) is leading cereal crop worldwide, but its yield is highly affected due to various diseases, especially Fusarium head blight (FHB), which affects the metabolism of plants. The present study was conducted at the Agricultural Institute Osijek using three winter wheat cultivars (Apache, Bezostaya1, and U1) during 2016/2017. The objectives of our studies were to examine differences in physiological characteristics of FHB resistance among wheat cultivars in the early stage of infection. The FHB incidence and severity was the highest in 'Bezostaya1'. Results suggest that activation of some anti-oxidative enzymes in the...
Calcium distribution during anther development in Impatiens balsaminaOriginal Papers
S.J. YANG, W.Y. LIANG, J. SHI, L. PENG, R. ZHENG
Biologia plantarum 64: 178-184, 2020 | DOI: 10.32615/bp.2019.147
The subcellular localization of loosely bound calcium in developing anthers of Impatiens balsamina (rose balzam) was investigated using potassium antimonate to precipitate the ions. At the sporogenous stage, few calcium precipitates were found in the young anthers. During meiosis of the microspore mother cell, the amount of calcium precipitates increased both inside these cells and in their cell walls. After meiosis, the resulting microspores accumulated abundant calcium precipitates in their cytoplasm and vacuoles. The microspores underwent mitosis to produce a bicellular pollen grain, in which the large vacuoles disintegrated, and the calcium...
Altered fatty acid composition of Nicotiana benthamiana and Nicotiana excelsior leaves under transient overexpression of the cyanobacterial desC geneOriginal Papers
M. BERESTOVOY, O.S. PAVLENKO, A.A. TYURIN, E.N. GORSHKOVA, I.V. GOLDENKOVA-PAVLOVA
Biologia plantarum 64: 167-177, 2020 | DOI: 10.32615/bp.2019.144
Transient heterologous gene expression in two model plant species, Nicotiana benthamiana and N. excelsior, has been used to study the localization of the heterologous Δ9 acyl-lipid desaturase (Δ9 desaturase) of Synechococcus vulcanus in different cell compartments and its functional activity in the cases of the cytosol, chloroplast, and endoplasmic reticulum (ER) localization. The functional activity and substrate specificity of the heterologous desaturase under the conditions of transient expression have been confirmed by comparison of fatty acid (FA) profiles. The Δ9 desaturase, responsible for the synthesis of...
Light and temperature receptors and their convergence in plantsOriginal Papers
J. SONG, W. WU, B. HU
Biologia plantarum 64: 159-166, 2020 | DOI: 10.32615/bp.2019.104
Light and temperature are two essential environmental cues for plants, helping to optimize plant body architecture and physiology. To sense a broad spectrum of sun radiation spanning from UV-B to far-red wavelength, plants are equipped with a sophisticated array of photoreceptors, including phytochromes, cryptochromes, phototropins, Zeitlupes, and UV-B photoreceptor UVR8. On the contrary, since the thermodynamic effects extensively affect the molecular and supramolecular structures, it is difficult to identify the entry point or initial receptor of temperature. Even so, several putative temperature sensors have been proposed, such as calcium ion channels,...
The enhancement of salt stress tolerance by salicylic acid pretreatment in Arabidopsis thalianaOriginal Papers
L.-L. YU, Y. LIU, F. ZHU, X.-X. GENG, Y. YANG, Z.-Q. HE, F. XU
Biologia plantarum 64: 150-158, 2020 | DOI: 10.32615/bp.2019.151
Salicylic acid (SA) is an important plant hormone involved in the activation of defense responses against environmental stresses. However, there are still large of unsolved mysteries about how SA pretreatment affects the establishment of plant stress tolerance. In this study, application of SA at different concentrations and different times were conducted to investigate their effects on the response of Arabidopsis seedlings to salt stress. The pretreatment with 10 or 20 μM SA for more than 6 h promoted Arabidopsis seedlings resistance to salt stress. On the other hand, pretreatment with 200 μM SA reduced Arabidopsis resistance...
Drought tolerance of transgenic rice overexpressing maize C4-PEPC gene related to increased anthocyanin synthesis regulated by sucrose and calciumOriginal Papers
Y.F. HE, Y.F. XIE, X. LI, J. YANG
Biologia plantarum 64: 136-149, 2020 | DOI: 10.32615/bp.2020.031
In order to reveal the role of sucrose (Suc) in early drought response in plants, transgenic rice (Oryza sativa L.) plants overexpressing the maize (Zea mays L.) C4-phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) gene (C4-pepc) (PC) and their untransformed wild type (WT) were used under 12 % (m/v) polyethylene glycol 6000 to simulate drought conditions. The results showed that PC has higher relative water content, the increased Suc content, and anthocyanin accumulation than WT during PEG treatment. By spraying 1 % Suc and 1 % Suc non-metabolic analog, turanose, on these plants, Suc...
Genome-wide transcriptome profiling provides new insights into bud dormancy in pearOriginal Papers
L.L. SUN, R. ZHANG, S.T. HE, J.P. LIU, G.Q. LIU
Biologia plantarum 64: 129-135, 2020 | DOI: 10.32615/bp.2019.116
Dormancy is important for the pear (Pyrus pyrifolia) to survive a harsh environment. The molecular base of dormancy in pear, especially in some local cultivars, is still unclear. Genome-wide transcriptome analysis in flower buds of cv. Huangli (an excellent local cultivar native to Guizhou mountain area in China) was conducted to explore the mechanism regulating bud dormancy in pear. For the release of endo-dormancy 223 chilling hours (CHs) was needed in Huangli flower buds, which was less than in commercial cultivars. Comparisons of transcript amounts among seven dates during dormancy (30 Oct. vs. 15 Nov., 15 Nov. vs. 30...
Grey poplar explant acclimation to improve the dehydration tolerance and cryopreservationOriginal Papers
E. POKORNÁ, M. FALTUS, P. MÁCHOVÁ, J. ZÁMEČNÍK, M. FULÍN
Biologia plantarum 64: 119-128, 2020 | DOI: 10.32615/bp.2019.148
Cryostorage techniques have been developed to preserve the most valuable genotype from an endangered native population of Populus × canescens Aiton Sm. (grey poplar), which is located in the floodplain forest in the South Moravia region of the Czech Republic and which is difficult to propagate using cuttings. The prevailing genotype with valuable traits was selected by the simple sequence repeats method. This genotype was used to determine the most effective pre-cultivation conditions (cold hardening, and cold hardening combine with osmotic treatment) on dehydration tolerance and post-thaw recovery of the grey poplar shoot tips. The pre-cultivation...
Di-n-butyl phthalate-induced phytotoxicity in Hordeum vulgare seedlings and subsequent antioxidant defense responseOriginal Papers
A. KUMARI, R. KAUR
Biologia plantarum 64: 110-118, 2020 | DOI: 10.32615/bp.2019.095
Di-n-butyl phthalate (DBP) is one of the frequently detected phthalates in environmental samples. The effects of phthalates are extensively studied in the animals but the effects on plants are scarce. Therefore, the present study is aimed to envisage the effects of DBP on the antioxidative defense system in Hordeum vulgare L. seedlings grown under laboratory conditions for 7 d. The activities of different antioxidative enzymes were enhanced in the shoots. In the roots, the activity of guaiacol peroxidase increased and the catalase activity decreased initially but increased at higher DBP concentrations, whereas the activities of superoxide...
High-throughput sequencing reveals the change of microRNA expression caused by allopolyploidization in CucumisOriginal Papers
X. YU, Z. ZHU, T. ZHANG, J. LI, C. CHENG, Q. LOU, C.-O. OTTOSEN, J. CHEN
Biologia plantarum 64: 104-109, 2020 | DOI: 10.32615/bp.2019.121
Allopolyploidy is long recognized as an essential driving force in plant evolution. Recent studies have demonstrated that small RNAs, including microRNAs (miRNAs), play important roles in the process of allopolyploidy. However, the question that how the distinct parent-of-origin miRNAs are maintained in allopolyploids and how these small RNAs affect gene expression and phenotype remain to be answered. Therefore, we investigated the miRNA expression profiles of a synthesized allotetraploid, Cucumis ×hytivus and its parents. The different developmental stages of leaves of C.×hytivus showing contrasting leaf colour were compared...
Three tandemly aligned LEA genes from Medicago truncatula confer differential protection to Escherichia coli against abiotic stressesOriginal Papers
Y.-M. ZHANG, H.-Q. WANG, D.-M. LIU, R.-J. LIU
Biologia plantarum 64: 95-103, 2020 | DOI: 10.32615/bp.2019.112
Late embryogenesis abundant (LEA) proteins are important for abiotic stress tolerance in diverse organisms. Within the LEA protein superfamily, group 4 members are characterized by a conserved N-terminal region and a structurally disordered C-terminal region that varies regarding length and amino acid content. Previous in vitro assays have suggested that the conserved N-terminal region shared by group 4 LEA proteins is critical for forming an amphipathic α-helix and protecting enzymatic activities from the adverse effects of desiccation or freezing. However, the cellular roles of the varying C-terminal region remain largely to be characterized....
Reactive oxygen species derived from NADPH oxidase regulate autophagyOriginal Papers
H. JING, Z. WANG, L. ZHANG, G. ZHOU, Z. GU, Y. SHAN, Y. ZHOU, Z. YANG, Z. KONG
Biologia plantarum 64: 87-94, 2020 | DOI: 10.32615/bp.2019.127
Reactive oxygen species (ROS) originating from nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) play vital roles in regulating autophagy. However, the relationship between autophagy and NOX in common wheat (Triticum aestivum L.) is still unknown. In order to clarify the mechanism of autophagy in wheat, ROS content, NOX activity, and autophagy levels in root tip cells under condition of N or C deficiency were measured. The results showed that the N and C deficiency increased the production of superoxide anions (O2-.) and hydrogen peroxide (H2O2) via the NOX activation, leading...
Pyramiding insect and disease resistance in an elite indica rice cultivar ASD16Original Papers
T. RAJESH, S. MARUTHASALAM, K. KALPANA, K. POOVANNAN, K.K. KUMAR, E. KOKILADEVI, D. SUDHAKAR, R. VELAZHAHAN, P. BALASUBRAMANIAN
Biologia plantarum 64: 77-86, 2020 | DOI: 10.32615/bp.2019.106
Pyramiding transgenes of interest is one of the strategies to engineer multiple stress resistance in crop plants. Transgenic plants which stably express different genes can be hybridized to bring these genes together in one plant. Transgenic rice (Oryza sativa L. cv. ASD 16) plants harbouring genes Xa21 (conferring bacterial blight resistance), tlp (conferring resistance to sheath blight), or gna (conferring resistance to brown planthopper) were used in hybridization experiments. Sexual hybridization was carried out in two different gene combinations: Xa21 × gna and tlp × gna. Molecular analyses were...
Genes involved in strigolactone biosyntheses and their expression analyses in columnar apple and standard appleOriginal Papers
X. SUN, C. WEN, H. HOU, H. HUO, J. ZHU, H. DAI, Y. ZHANG
Biologia plantarum 64: 68-76, 2020 | DOI: 10.32615/bp.2019.047
Columnar apple is a valuable resource for genetic improvement of cultivated apples due to its special tree architecture. Strigolactones (SLs) are a novel class of plant hormones controlling shoot branching. The content of SLs is higher in columnar apple than in standard apples. In this study, the members of major gene families involved in SLs biosynthesis and signaling were identified from apple genomic sequences and their expression profiles were characterized in columnar and standard apples using reverse transcription quantitative polymerase chain reactions. In comparison with standard apple, the higher expressions of MORE AXILLARY GROWTH...
Overexpression of oil palm EgDREB1 in tomato decreased fruit size and produced parthenocarpic fruitsOriginal Papers
A.M. AZZEME, S.N.A. ABDULLAH, M.A. AZIZ, P.E. MEGAT WAHAB
Biologia plantarum 64: 58-67, 2020 | DOI: 10.32615/bp.2019.084
Drought-responsive element binding (DREB) is involved in the regulation of stress-responsive gene expressions in plants through abscisic acid (ABA)-independent pathway. In this study, constitutive expression of oil palm (Elaeis guineensis) EgDREB1 driven by double strength cauliflower mosaic virus 35S promoter in tomato (Solanum lycopersicum) reduced seed number, produced parthenocarpic fruits, changed morphology of leaves, and increased root biomass of transgenic plants. Early flowering and fruiting of the transgenic lines were observed in the culture vessels. EgDREB1 was specifically expressed in the fruits and its expression...
Overexpression of Iris lactea tonoplast Na+/H+ antiporter gene IlNHX confers improved salt tolerance in tobaccoOriginal Papers
Q. GUO, X.X. TIAN, P.C. MAO, L. MENG
Biologia plantarum 64: 50-57, 2020 | DOI: 10.32615/bp.2019.126
Sodium cation compartmentalization into vacuoles is one of the effective strategies for adaptation of halophytes to saline environments. Tonoplast Na+/H+ antiporter (NHX) is involved in Na+ sequestration into vacuoles under salt stress. However, the function of NHX in halophyte Iris lactea is still unclear. In this study, a significant positive correlation was observed between Na+ accumulations and IlNHX expression in tissues under 0 - 200 mM NaCl, indicating IlNHX might be responsible for Na+ accumulation of I. lactea under salt stress. More important, IlNHX was specifically...
Changes of lipid metabolism of Arabidopsis thaliana in response to oligochitosan treatmentOriginal Papers
X.J. WANG, X.L. SU, J.M. LI, P. ZHU
Biologia plantarum 64: 43-49, 2020 | DOI: 10.32615/bp.2019.133
Lipids are vital cellular constituents in plant, and lipid peroxidation metabolites are critical defence substances in plants. In this study, mass spectrometry along with projections to latent structures discriminant analysis (PLS-DA) was used to detect lipid metabolism changes in Arabidopsis thaliana in response to oligochitosan (an effective resistance elicitor for the control of plant diseases). The PLS-DA showed that lipid metabolites of Arabidopsis thaliana were influenced by oligochitosan treatment. The total content of oxylipin containing monogalactosyldiacylglycerols, oxylipin-containing digalactosyldiacylglycerols, and oxylipin-containing...
Treatment of Glycine max seeds with gibberellins alters root morphology, anatomy, and transcriptional networksOriginal Papers
Y.Q. HAN, Y. SHI, Y.M. GAO, J.D. DU, N.J. FENG, Y.X. ZHANG, D.F. ZHENG
Biologia plantarum 64: 32-42, 2020 | DOI: 10.32615/bp.2019.124
Gibberellins (GAs) regulate diverse aspects of growth and development, but their role in root development and lateral root (LR) formation is poorly understood. In this study, GA3 was applied to soybean [Glycine max (L.) Merr] by seed soaking. The results showed that root length and root surface area were significantly inhibited in early stages after GA3 treatment. Microscopic examination showed that GA3 treatment changed the cortex thickness, the pericycle diameter, and cell size in main root. Interestingly, exogenous GA3 increased the quantity of lateral root primordia (LRP), but LR number decreased...
Biochemical and morphophysiological strategies of MyracrodruonOriginal Papers
L.M. SOUZA, M.R. BARBOSA, M.B. MORAIS, L. PALHARES NETO, C. ULISSES, and T.R. CAMARA
Biologia plantarum 64: 20-31, 2020 | DOI: 10.32615/bp.2019.070
In view of the ecological, social, and economic importance of Myracrodruon urundeuva Allemão, the objective of this study was to investigate the strategies of this species under drought during its initial phase of development. Two-month-old plants were cultivated under continuous irrigation or no irrigation for 20 d. After this period, the water-stressed plants were rehydrated for 20 d. Physiological, biochemical, and anatomical variables were evaluated on 20th and 40th day. Water deficit (25 and 85 % leaf relative content) caused senescence followed by leaf abscission. Growth in height was negatively affected by water...
Revision of the barley WRKY gene family phylogeny and expression analysis of the candidate genes in response to droughtOriginal Papers
B. YAZDANI, S. SANJARI, R. ASGHARI-ZAKARIA, F. GHANEGOLMOHAMMADI, E. POURABED, M. SHAHBAZI, Z.-S. SHOBBAR
Biologia plantarum 64: 9-19, 2020 | DOI: 10.32615/bp.2019.109
The WRKY belongs to an important plant specific transcription factor families which are involved in response to various environmental stresses, as well as in growth and developmental processes. In the present report, a genome-wide identification and characterization of WRKY gene family in barley led to revision of HvWRKYs to 93 members. The phylogenetic tree was also reconstructed based on the full-length WRKY protein sequences in barley and Arabidopsis. HvWRKYs were classified into three major groups (I, II, and III) and group II was further divided to 5 subgroups (a to e). HvWRKYs were named after this classification. Interestingly,...
Silver nanoparticles with different concentrations and particle sizes affect the functional traits of wheatOriginal Papers
S. WANG, B. D. WU, M. WEI, J. W. ZHOU, K. JIANG, C.Y. WANG
Biologia plantarum 64: 1-8, 2020 | DOI: 10.32615/bp.2019.122
The response of functional traits of plants to external environment can influence their competitive ability because these functional traits are required for the acquisition of resources. The overuse of silver nanoparticles (AgNPs) has gained attention due to their environmental toxicity. This study aimed to examine the effects of AgNPs with different concentrations and particle sizes on functional traits of wheat. It was observed that AgNPs significantly reduced the plant height and so decrease its competitive ability. Ag ions decreased leaf chlorophyll and nitrogen content and specific leaf area more than AgNPs, but the opposite was true for leaf...
Transcriptome sequencing flower petals reveals insights into regulation of flavonoid biosynthesis in Osmanthus fragransOriginal Papers
Y.J. HAN*, M.F. DONG, H.Y. WANG, X.D. WANG, K. LI, F.D. SHANG*
Biologia Plantarum 63: 765-775, 2019 | DOI: 10.32615/bp.2019.146
Osmanthus fragrans Lour., one of the top 10 most popular flowers in China, is known for both its beauty and fragrance. It is rich in flavonoids, a class of secondary metabolites with significant neuroprotective, free-radical scavenging, and anti-oxidant activity. To understand the mechanisms regulating flavonoid biosynthesis, we conducted transcriptome sequencing O. fragrans flowers to analyze gene expressions during the full flowering stage. The RNA was isolated separately from petals of cvs. Yingui and Dangui, which were treated or not with jasmonic acid, salicylic acid, or abscisic acid. A total of 142 029 unigenes were denovo assembled,...
A methyl jasmonate induced defensin like protein from Panax notoginseng confers resistance against Fusarium solani in transgenic tobaccoOriginal Papers
Q. WANG, B.L. QIU, S. LI, Y.P. ZHANG, X.M. CUI, F. GE, D.Q. LIU
Biologia Plantarum 63: 797-807, 2019 | DOI: 10.32615/bp.2019.123
Plant defensins and defensin like protein (DEFL) form a large family of small cysteine-rich proteins. They are major components of plant immune systems, being involved in host defenses against biotic and abiotic stresses. In this study, a novel defensin like protein (DEFL) gene PnDEFL1 was isolated from Panax notoginseng, a traditional Chinese medicinal herb. The expression patterns of PnDEFL1 after treatment with methyl jasmonate, salicylic acid, ethephon, and H2O2, as well as during Fusarium solani infection, were analyzed using reverse transcription qPCR. The up-regulated expression of PnDEFL1...
Promoter activity of genes encoding the Specific Tissue protein family in the reproductive organs of Medicago truncatulaOriginal Papers
L. ALBORNOS, I. MARTÍN, E. LABRADOR*, B. DOPICO
Biologia Plantarum 63: 785-796, 2019 | DOI: 10.32615/bp.2019.111
The "Specific Tissue" (ST) are proteins of unknown function present only in some plant families, mainly Fabaceae and Asteraceae. They are included in the PF10950 protein family and characterized by the presence of at least one domain of unknown function (DUF)2775. In this work we studied the involvement of the six members of the Medicago truncatula ST family (ST1 to ST6) in the development of flowers, fruits, and seeds by analysing the activity of their promoters (pST) after the construction of M. truncatula transgenic plants expressing the b-glucuronidase (GUS) reporter gene under the control of the six pSTs....
An overexpression of the AP2/ERF transcription factor from Iris typhifolia in Arabidopsis thaliana confers tolerance to salt stressOriginal Papers
J. WU, J. ZHANG, X. LI, J. LIU, Z. NIU, L. WANG*
Biologia Plantarum 63: 776-784, 2019 | DOI: 10.32615/bp.2019.082
The roles of ethylene responsive factors (ERFs) and their positive and negative regulations of abiotic stress tolerance have been widely reported. This study reports the characterization of ItERF from Iris typhifolia Kitag with respect to molecular and functional properties. The 867 bp cDNA fragment of ItERF was cloned by reverse transcription PCR from I. typhifolia. Real-time quantitative PCR revealed that ItERF expression was induced in the roots, stems, and leaves of I. typhifolia after NaCl treatment, and that ItERF expressions were significantly higher in the leaves and roots than in the stems....
Proline metabolism-related gene expression in four potato genotypes in response to drought stressOriginal Papers
Y. LIU, L. WANG, Y. LI, X. LI, J. ZHANG
Biologia Plantarum 63: 757-764, 2019 | DOI: 10.32615/bp.2019.153
Drought severely limits potato yield. The aim of this work was to study a response of plantlets of four potato genotypes to polyethylene glycol (PEG 6000)-induced drought stress in both physiological and molecular levels. The drought-tolerant and drought-sensitive genotypes were identified based on plantlet growth, chlorophyll content, lipid peroxidation, free proline content, and proline metabolism-related gene expressions. We found that PEG-induced stress increased malondialdehyde (MDA) and proline content, and drought-tolerant plantlets exhibited lower MDA and proline content than sensitive genotypes. In addition, PEG up-regulated ∆-1-pyrroline-5-carboxylate...
Constitutive expression of the wheat TaSOD5 gene enhances salinity tolerance of Arabidopsis thalianaOriginal Papers
Y.-G. SONG, T.-X. GAO, X.-J. LIU, W. DONG*
Biologia Plantarum 63: 750-756, 2019 | DOI: 10.32615/bp.2019.108
Superoxide dismutase is a crucial reactive oxygen species (ROS) scavenger and converts the superoxide radical (O2-) to H2O2, so it is thought to enhance abiotic stress tolerance by reducing ROS accumulation and so avoiding oxidative damage. In this study, we isolated a salt- and oxidative stress-responsive Cu/Zn SOD gene TaSOD5 from wheat. The ectopic overexpression of TaSOD5 in Arabidopsis increased total and Cu/Zn SOD activities, and offered the plant tolerance to salt stress. Arabidopsis ectopically expressing TaSOD5 possessed a superior resistance to oxidative stress...
Proteome analysis of sesame leaves in response to waterlogging stress at vegetative and flowering stagesOriginal Papers
H.-J. JUNG, S.K. ROY, S.-W. CHO, S.-J. KWON, C. KUN, H.-C. CHUN, S.-H. WOO
Biologia Plantarum 63: 733-749, 2019 | DOI: 10.32615/bp.2019.062
Waterlogging, a major environmental stress, impairs plant growth and development and induces synthesis of different proteins. To understand the molecular mechanisms coupled with morpho-physiological alterations underlying waterlogging tolerance, the LTQ-FTICR MS/MS technique was employed to map the proteomes of leaves of sesame grown under control and waterlogged conditions. The waterlogging treatment caused dramatic alterations in morphological and biochemical properties of the leaves of sesame. For proteome analysis, more than 75 reproducible protein spots were identified on 2-DE gels wherein 51 protein spots (≥ 1.5-fold change) were used for...