Photosynthetica - Ahead of Print
Special issue in honour of Prof. Reto J. Strasser – Development and aging of photosynthetic apparatus of Vitis vinifera L. during growing season
K. SITKO, S. RUSINOWSKI, M. POGRZEBA, A. DASZKOWSKA-GOLEC, Ż. GIEROŃ, H.M. KALAJI, E. MAŁKOWSKI
Photosynthetica X:X | DOI: 10.32615/ps.2019.107
The aim of this study was to examine the development and aging of chosen grapevine leaves in situ during the growing season (130 d) using chlorophyll (Chl) a fluorescence measurements and determining the changes in pigment contents. During the course of photosystems development, the increase of Chl and decrease of anthocyanin contents in leaves was observed simultaneously. On 28th day, the maximum content of Chl and minimum content of anthocyanins was measured. However, the maximal photosynthetic performance was found one week later, when the content of Chl started to diminish. Our study proved that the achievement of maximal...
Special issue in honour of Prof. Reto J. Strasser – Plant biomass in salt-stressed young maize plants can be modelledwith photosynthetic performance
V. GALIĆ, M. MAZUR, D. ŠIMIĆ, Z. ZDUNIĆ, M. FRANIĆ
Photosynthetica X:X | DOI: 10.32615/ps.2019.131
Predicting responses to stressful conditions is very important. Chlorophyll a fluorescence (ChlF) can be used to assess effects of various stresses on photosynthetic performance. We tested the responses of five 10-d old maize hybrids to salinity stress by measuring ChlF parameters, fresh (FM) and dry mass (DM). ChlF data were incorporated into a penalized regression model to predict biomass traits. The values of FM and DM significantly decreased under salt stress by 42 and 25%, respectively. Strong responses in ChlF parameters assessing the absorption dissipation and trapping fluxes to NaCl treatment were detected. In penalized regression models,...
Special issue in honour of Prof. Reto J. Strasser – Structural and functional response of photosynthetic apparatus of radish plants to iron deficiency
I.A. SAMBORSKA-SKUTNIK, H.M. KALAJI, L. SIECZKO, W. BĄBA
Photosynthetica X:X | DOI: 10.32615/ps.2019.132
In this work, we tried to identify some specific chlorophyll a fluorescence (ChlF) parameters, that could enable detection of iron deficiency (Fedef) in radish plants (Raphanus sativus L.), before any visual symptoms appear. Changes in ChlF kinetics, JIP-test parameters, and chlorophyll content revealed that iron deficiency negatively affected PSII activity mainly via disruption of light absorption in light-harvesting complexes and by decreasing the activity of the primary quinone acceptor of PSII (QA). Iron deficiency was clearly reflected in the changes of some JIP-test parameters, such as time to reach...
Photosynthetic characteristics and chlorophyll a fluorescence induction parameters in elite clone (GS1) of Pinus sylvestris var. mongolica
P. MENG, G.C. YOU
Photosynthetica X:X | DOI: 10.32615/ps.2019.133
We selected the elite clone of Pinus sylvestris var. mongolica 'GS1' from 365 clones in a primary seed orchard in Liaoning Province, China. To understand the photosynthesis characteristics and mechanisms, 40-year-old 'GS1' trees were compared with a common clone 'H524' of the same age at the same seed orchard. Results showed that 'GS1' had higher net photosynthetic rate because of higher stomatal conductance (gs); the larger external and inner diameters of stomata were the structural factors enabling maintenance of higher gs. The higher light-saturated net photosynthetic rate of 'GS1' was mainly due...
Leaf photosynthetic light response of summer maize: comparison of models and analysis of parameters
T.T. ZHU, J. LI, Y.Y. LIU, X.J. TONG, Q. YU
Photosynthetica X:X | DOI: 10.32615/ps.2019.134
An experiment was performed in order to study the applicability of light-response models for summer maize (Zea mays L.) by using rectangular hyperbola model, nonrectangular hyperbola model, exponential model, binomial regression model, and modified rectangular hyperbola model (Ye model), respectively. Our results showed that the fitted effect of Ye model was best and photosynthetic parameters were closest to the observation. The photoinhibition coefficient was significantly negatively correlated with the light-saturation point (Is), the light-saturation coefficient (γ), and maximum net photosynthetic rate at light-saturation...
Inhibition of photosynthesis in cucumber leaves by oxybenzone
X. ZHONG, Y.T. LI, Z.S. ZHANG, S.H. LI, Y.M. LI, B.B. LIU, Q.M. LI, H.Y. GAO
Photosynthetica X:X | DOI: 10.32615/ps.2019.135
Oxybenzone (OBZ), a popular additive in sunscreen, can protect human skin by blocking ultraviolet (UV) rays from the sun (Baker et al. 2015). In addition to being used in sunscreens, OBZ is also widely used in perfumes, shampoos, lip balms, and other pharmaceuticals and personal care products (PPCPs) (Aguirre et al. 1992, Emonet et al. 2001, Kasichayanula et al. 2007). OBZ can enter rivers, lakes, groundwater, sewage, and so on through human activities and was identified as an 'emerging environmental pollutant' by the Environmental Protection Agency of the USA (Rodil et al. 2012, Agüera et al. 2013). With...
Ultraviolet (UV) B effects on growth and yield of three contrasting sweet potato cultivars
Z. CHEN, W. Gao, K.R. Reddy, M. Chen, S. Taduri, S.L. Meyers, M.W. Shankle
Photosynthetica X:X | DOI: 10.32615/ps.2019.137
Ground-level UV-B will stay at a high level in the next several decades and influence sweet potato growth and yield because of the remaining chlorofluorocarbons in the atmosphere. The study explored three UV-B (none, ambient, and elevated/projected) levels on three contrasting sweet potato cultivars (Beauregard, Hatteras, and Louisiana 1188) using sunlit plant growth chambers at Mississippi State University. The results showed that UV-B influenced three cultivars differently. Growth, photosynthetic rate, epidermal and leaf structure of Beauregard were negatively influenced under ambient and elevated UV-B. On the contrary, Hatteras was positively influenced,...
Special issue in honour of Prof. Reto J. Strasser – Photoinhibition of oxygen-evolving complex and photosystem II at chilling stress in the tropical tree species Dalbergia odorifera
Y.-J. YANG, T. LIU, S.-B. ZHANG, W. HUANG
Photosynthetica X:X | DOI: 10.32615/ps.2019.138
Photosystem II is sensitive to chilling stress in tropical tree species. However, the underlying mechanism is not clear. In this study, we examined the effects of chilling and light stress on activities of PSII and oxygen-evolving complex (OEC), in order to resolve the controversy of whether chilling-induced PSII photoinhibition is attributed to the excess-energy model or the two-step scheme. We determined changes in chlorophyll a fluorescence transient and energy distribution in PSII during treatment at chilling-light stress for the tropical tree species Dalbergia odorifera. At chilling temperature, the light-use efficiency was largely...
Photosynthesis, growth, and yield of Paeonia ostii in tree-based agroforestry systems
Y.ZH. YANG, ZH.H. ZHANG, G.X. WANG, Y.Y. YANG, H. GUO
Photosynthetica X:X | DOI: 10.32615/ps.2019.139
Paeonia ostii T. Hong & J.X. Zhang, an emerging oil crop, was intercropped with 3-year-old plantations of pawpaw [Chaenomeles sinensis (Thouin.) Koehne], Chinese toon (Toona sinensis Roem.), and walnut (Juglans regia L.). In order to achieve a better production system, we studied the effects of intercropping on the photosynthesis, growth, and yield of P. ostii. The results showed that different pattern of agroforestry systems changed microclimatic and growing site conditions in comparison to the control treatment. The correlation analysis demonstrated that both similarities and differences were found in relationship...
Photosynthetic activity of common buckwheat (Fagopyrum esculentum Moench) exposed to thermal stress
M. HORNYÁK, A. PŁAŻEK, P. KOPEĆ, M. DZIURKA, J. PASTUSZAK, A. SZCZERBA, T. HURA
Photosynthetica X:X | DOI: 10.32615/ps.2019.140
The aim of the work was to investigate thermal stress effect on photosynthetic activity of common buckwheat. Seedlings of common buckwheat were exposed to 20°C (control) and 30°C (thermal stress). The research involved the Polish cultivar 'Panda' and strain PA15 and determined kinetics of chlorophyll a fluorescence (Chlf), leaf gas exchange, soluble carbohydrate (SC) content in donor leaves, electrolyte leakage as a parameter of cell membrane permeability, and amount of abscisic acid and jasmonates. In 'Panda' and PA15 plants grown at 30°C, most of Chlf parameters improved. 'Panda' plants grown at 30°C demonstrated a higher increase in...
Triadimefon increases drought tolerance through the regulation of photosynthesis and carbohydrate metabolism in rapeseed at bolting stage
F. WANG, F. ZHONG, S. ZHANG, P. ZHANG, F. CHEN, W. LI, S. ZHANG, H. JIANG
Photosynthetica X:X | DOI: 10.32615/ps.2019.141
Drought is a major abiotic factor limiting agricultural crop production. The focus of this study was the effect of triadimefon (TDM) on rapeseed photosynthesis and carbohydrate metabolism in response to drought stress. Results showed that TDM increased plant dry mass per plant and reduced the damage to photosynthetic processes by regulating stomatal and nonstomatal factors, inducing photosynthetic pigment synthesis, and improving photosynthetic activity. Chloroplast degradation and senescence was reduced in rapeseed leaves with TDM under drought stress. TDM restored structural connections between chloroplasts and cell membranes and modified the chloroplasts...
Special issue in honour of Prof. Reto J. Strasser – Photosynthesis response of microalgae (Tetradesmus wisconsinensis) to different inorganic carbon sources probed with chlorophyll fluorescence analysis
E. JANKA, I. UMETANI, M. SPOSOB, R. BAKKE
Photosynthetica X:X | DOI: 10.32615/ps.2019.142
Bicarbonate is an alternative carbon source for production of photosynthetic microalgae, although sparging of CO2-enriched gas is commonly practised, its efficiency seems to be scarcely considered. This study evaluated the photosynthetic activities of microalgae (Tetradesmus wisconsinensis) using chlorophyll fluorescence under different concentrations of bicarbonate (HCO3-) and CO2. Chlorophyll fluorescence analysis showed noticeable difference in photosynthetic activity between the bicarbonate (HCO3-) and CO2 supplied cultures. The JIP-test parameters obtained...
Special issue in honour of Prof. Reto J. Strasser – Chlorophyll fluorescence, leaf gas exchange, and genomic analysis of chromosome segment substitution rice lines exposed to drought stress
K. HUNGSAPRUG, T. KOJONNA, M. SAMLEEPAN, C. PUNCHKHON, W. UT-KHAO, B. KOSITSUP, W. KASETTRANUN, J.L. SIANGLIW, T. TOOJINDA, L. COMAI, K. PLAIMAS, S. CHADCHAWAN
Photosynthetica X:X | DOI: 10.32615/ps.2019.144
This research aims to evaluate the photosynthesis-related parameters in rice chromosome segment substitution lines (CSSL), containing drought-tolerant region from DH212 in a Khao Dawk Mali105 genetic background. Screening at seedling stage indicated that CSSL4 was more tolerant to drought stress than KDML105 with the higher maximal quantum yield of PSII photochemistry. After withholding water, the decline in light-saturated net photosynthetic rate due to drought stress occurred simultaneously with the decrease in electron transport rate and effective quantum yield of PSII photochemistry values, suggesting that stomatal changes affect light-saturated...
Special issue in honour of Prof. Reto J. Strasser – The photosynthetic performance of two Citrus species under long-term aluminum treatment
H. ZHANG, X.Y. LI, L.-S. CHEN, Z.R. HUANG
Photosynthetica X:X | DOI: 10.32615/ps.2019.145
The effect of Al stress on leaf pigment contents, chlorophyll (Chl) a fluorescence transient, and leaf gas exchange of Citrus sinensis and Citrus grandis were investigated in sandy culture with nutrient solution (control) or supplemented by 0.5 mM AlCl3.6H2O (Al toxic) for 54 weeks. We found a significant decline of Chl a, carotenoids (Car), Ch a/b, and Car/Chl (a+b) in C. grandis caused by Al stress. Except the significant increase of Car/Chl (a+b), no remarkable difference was found in C. sinensis. The Al-induced downregulation of CO2...
Special issue in honour of Prof. Reto J. Strasser – Chlorophyll a fluorescence of Bertholletia excelsa Bonpl. plantations under thinning, liming, and phosphorus fertilization
K.C. PIRES DA COSTA, R. KIRMAYR JAQUETTI, J.F. DE CARVALHO GONÇALVES
Photosynthetica X:X | DOI: 10.32615/ps.2019.146
Phosphorus (P) fertilization and liming can reduce negative effects caused by a sudden increase in light availability after thinning of forest plantations. In this study, immediately after thinning, photochemical performance (quantum yield of PSII and performance index) decreased, however, liming and P fertilization reduced the negative effects caused by thinning and accelerated the recovery of Bertholletia excelsa trees. After thinning, the remaining trees increased J-I phase with the formation of a positive K-band which occurred exclusively in unfertilized (no liming or no P) individuals. Additionally, enhanced effective dissipation (DI0/RC)...
Special issue in honour of Prof. Reto J. Strasser – Chlorophyll a fluorescence parameters as indicators of a particular abiotic stress in rice
P. FASEELA, A.K. SINISHA, M. BRESTIČ, J.T. PUTHUR
Photosynthetica X:X | DOI: 10.32615/ps.2019.147
The aim of this study was to evaluate the validity of some chlorophyll (Chl) a fluorescence parameters as early indicators of a particular abiotic stress and also to characterize the effect of different abiotic stresses [high light, NaCl, polyethylene glycol (PEG)-induced osmotic stress, and heavy metals] on the electron transport chain of rice (Oryza sativa L.) seedlings. The results clearly revealed that Chl a fluorescence parameters differ between abiotic stress types and also allowed us to select some parameters which were specifically and intensively affected under different abiotic stresses. We observed that the performance...
Special issue in honour of Prof. Reto J. Strasser – A comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass)
B. JIMÉNEZ-FRANCISCO, A. STIRBET, H. CAMPOS, F.V. CONDE-MARTÍNEZ, D. PADILLA-CHACÓN, C. TREJO, C.J. BERNACCHI, G. GOVINDJEE
Photosynthetica X:X | DOI: 10.32615/ps.2019.148
Bouteloua gracilis (blue grama grass) is one of the most drought and grazing tolerant plants in the short-grass ecosystem. To obtain information on their photosystem activities, we measured the fast (< 1 s) chlorophyll a fluorescence transient (the OJIP curve) from their leaves, and isolated cells grown photoautotrophically in suspension in a culture medium, or with added sucrose. One of our goals was to study the effect of different sucrose concentrations (0, 0.15, 0.3, and 3%) on PSII activity in isolated cells. Our results on cells suspended in culture medium, using the JIP-test, showed a decrease in PSII activity at increasing sucrose...
Special issue in honour of Prof. Reto J. Strasser – Can be performance indexes used to select plant growth-promoting rhizobacteria?
A.T. PERBONI, E.G. MARTINAZZO, A.B. MOURA, M.A. BACARIN
Photosynthetica X:X | DOI: 10.32615/ps.2019.149
The plant growth-promoting rhizobacteria (PGPR) are bacteria that colonize the rhizosphere or roots, resulting in a beneficial effect on plants. The evaluation of the PGPR effects is mostly based on growth biometric measurements, but there is little information about using the chlorophyll (Chl) fluorescence. We used rhizobacteria to treat canola seeds and evaluate their effects on leaf area, dry mass, and Chl fluorescence. In order to select PGPR, we suggest the JIP-test as a method to evaluate their effects using the performance indexes. The biometric measurements and JIP-test parameters allowed to group bacterial isolates into different groups. We...
Special issue in honour of Prof. Reto J. Strasser – Revisiting JIP-test: An educative review on concepts, assumptions, approximations, definitions and terminology
M. TSIMILLI-MICHAEL
Photosynthetica X:X | DOI: 10.32615/ps.2019.150
The aim of this educative review is, by revisiting the JIP-test as introduced and further elaborated and extended by Professor Reto J. Strasser and his research group, to clarify concepts, assumptions, and approximations on which it is based, as well as definitions and terms it uses, reminding that it is meant to evaluate impacts of environmental stresses -factors and/or perturbations - on the photosynthetic structure and function. It analyses how the JIP-test, based on the Theory of Energy Fluxes and adopting Duysens' concept, translates the OJIP polyphasic chlorophyll a fluorescence rise kinetics, emitted by PSII, into biophysical parameters...
Special issue in honour of Prof. Reto J. StrasserProbing the photosynthetic efficiency of some European and Anatolian Scots pine populations under UV-B radiation using polyphasic chlorophyll a fluorescence transient
N. ÇİÇEK, H.M. KALAJI, Y. EKMEKÇİ
Photosynthetica X:X | DOI: 10.32615/ps.2019.151
The study was carried out to examine the photosynthetic efficiency of Scot pine populations, originated from various European countries and Anatolia in Turkey, exposed to UV-B radiation. Seeds were germinated and grown in the nursery; three-year-old seedlings were exposed to UV-B for two days, eight hours per day. The photosynthetic efficiency was probed by chlorophyll a fluorescence at 24 h after UV-B treatment and analysed by JIP-test. UV-B caused reduction in quantum yields and electron transport at both donor and acceptor sides of photosystems. The performance indexes of seedlings were also negatively affected by the treatment. Populations...
Photosynthetic and physiological responses of small seeded faba bean genotypes (Vicia faba L.) to salinity stress: identification of a contrasting pair towards salinity
I. RAJHI, S. BEN MOUSSA, I. NEJI, B. BACCOURI, M. BEN CHIKHA, C. CHAMMAKHI, M. AMRI, R. BROUQUISSE, H. MHADHBI
Photosynthetica X:X
In this study, we examined the behavior of six faba bean (Vicia faba L.) genotypes under salinity conditions at the seedling stage. The evaluation was assessed using 21 morphological, physiological, and photosynthetic traits under control, moderate, and severe salinity conditions. The principal component analysis (PCA) was used to identify genotypes of contrasting behavior in response to salt stress and to characterize the important traits for salt tolerance. The result of PCA analysis showed that the genotypes displayed different behavior under the saline conditions. The most discriminating quantitative characters were related to plant biomass...
N-glycosylation regulates photosynthetic efficiency of Arabidopsis thaliana
Q.-S. JIAO, G.-T. NIU, F.-F. WANG, J.-Y. DONG, T.-S. CHEN, C.-F. ZHOU, Z. HONG
Photosynthetica X:X | DOI: 10.32615/ps.2019.153
N-glycosylation is one of the most important protein modifications in eukaryotes. It has been well established that N-glycosylation plays multiple roles in regulating stress tolerance of plants. However, the effects and mechanism of N-glycosylation on photosynthesis have not been well understood. In the present study, an obvious decrease in photosynthetic capacity and dry mass were detected in alg3-3 and cgl1-1, two typical mutants in N-glycosylation process. The maximal photochemical efficiency of PSII decreased significantly in cgl1-1. The values of effective quantum yield of PSII photochemistry, rate of photosynthetic electron...
Co-author and co-cited reference network analysis for chlorophyll fluorescence research from 1991 to 2018
K. HU, G. GOVINDJEE, J. TAN, Q. XIA, Z. DAI, Y. GUO
Photosynthetica X:X | DOI: 10.32615/ps.2019.154
Chlorophyll a fluorescence has been extensively used in studying photosynthesis and overall physiology of plants, algae, and cyanobacteria. As a research hotspot, a large number of papers have been published. This creates a challenge for beginning researchers to gain a holistic view of co-author and co-cited reference network by reading individual publications. Further, these scientists need to understand the research trends in the field. Scientometric analysis, a method for mining publication datasets, was therefore conducted to analyze the co-author and co-cited reference network in the area of chlorophyll fluorescence research. The countries...
Toxic effects of potassium permanganate on photosystem II activity of cyanobacteria Microcystis aeruginosa
L. LI, X.L. PAN, G.J. MU
Photosynthetica X:X | DOI: 10.32615/ps.2019.155
Effects of potassium permanganate (KMnO4) on PSII of Mycrocystis aeruginosa were investigated by measuring the chlorophyll fluorescence in vivo. KMnO4 exposure reduced the rate of oxygen evolution and cell growth. High concentration of KMnO4 (10 mg L-1) decreased the fast phase and increased the slow phase of QA- reoxidation kinetics. Electron transport after QA was blocked, resulting in a considerable amount of QA- reoxidation being performed via S2(QAQB)- charge recombination. KMnO4...
Different responses of photosystem and antioxidant defense system to three environmental stresses in wheat seedlings
N. WU, H.T. MAO, M.Y. CHEN, J. DONG, M. YUAN, Z.W. ZHANG, S. YUAN, H.Y. ZHANG, Y.E. CHEN
Photosynthetica X:X | DOI: 10.32615/ps.2019.156
To investigate the adaptive mechanism of wheat under high light, osmotic stress, and salt stress, redox regulation, and photosynthesis were compared. Under high light, the activities of antioxidant enzymes, except for catalase, significantly increased and then decreased after 1 and 3 h, respectively. Under osmotic stress, antioxidant enzyme activities significantly increased and subsequently declined. Except for superoxide dismutase, salt stress induced a significant increase in all the antioxidant enzyme activities. A significant decrease of D1 protein by high light and osmotic stress, strong photophosphorylation of D1 and D2 under high light and...
Nitric oxide participates in the regulation of ascorbate-glutathione cycle and water physiological characteristics of Arabidopsis thaliana by NaHS
C.J. SHAN, Y.Y. JIN, Y. ZHOU, H. LI
Photosynthetica X:X | DOI: 10.32615/ps.2019.158
We investigated the role of nitric oxide (NO) in the regulation of ascorbate-glutathione (AsA-GSH) cycle and water physiological characteristics of Arabidopsis thaliana by sodium hydrosulfide (NaHS). NaHS markedly increased the contents of H2S, NO, chlorophyll (Chl), and carotenoids, the activity of AsA-GSH cycle, ascorbate/dehydroascorbate ratio, net photosynthetic rate, Chl fluorescence parameters, transpiration rate, stomatal conductance, and relative water content in leaves and the biomass of wild-type Arabidopsis. However, NaHS markedly decreased malondialdehyde content and electrolytic leakage. Except H2S,...
Special issue in honour of Prof. Reto J. Strasser – JIP-test parameters to study apple peel photosystem II behavior under high solar radiation stress during fruit development
V. BLACKHALL, G.A. ORIOLI, M.G. COLAVITA
Photosynthetica X:X | DOI: 10.32615/ps.2019.159
Changes in apple (Malus domestica Bork.) peel PSII photochemical process during fruit development under high solar radiation field conditions were analysed by the JIP-test to increase understanding on energy dissipation and susceptibility to photooxidative damage. Fruits growing exposed (E), non-exposed (NE) or suddenly exposed (SU) to high solar radiation were evaluated at three developmental stages. Chlorophyll content and chlorophyll a fluorescence transient (OJIP), were affected by high solar radiation and development. Minimum fluorescence, maximum fluorescence, maximum quantum yield of photochemistry, and specific fluxes per cross...
Exogenous spermidine enhances salt-stressed rice photosynthetic performance by stabilizing structure and function of chloroplastand thylakoid membranes
D.X. JIANG, X. CHU, M. LI, J.J. HOU, X. TONG, Z.P. GAO, G.X. CHEN
Photosynthetica X:X | DOI: 10.32615/ps.2019.160
Spermidine (Spd) is a ubiquitous low-molecular-mass aliphatic amine that acts in abiotic stress tolerance in plants. We investigated how the treatment with exogenous Spd contributed to the protection against salt stress in rice chloroplasts. Analysis of the chlorophyll (Chl) fluorescence showed that there were many negative effects of salinity on different sites of the photosynthetic machinery; however, these were alleviated by adding Spd. Spd prevented the damage of structure and function of chloroplasts under salt stress; thylakoid membrane protein components and photosynthetic pigments were not severely affected by salinity in Spd-treated plants....
Special issue in honour of Prof. Reto J. Strasser – Seasonal variation and trade-off between frost resistance and photosynthetic performance in woody species
A. GAST, C. RÖMERMANN, S.F. BUCHER
Photosynthetica X:X | DOI: 10.32615/ps.2019.161
We tested the seasonal variability of frost resistance and photosynthesis characteristics, namely net photosynthetic rate at saturating irradiance and ambient atmospheric CO2 concentrations, maximum carboxylation rate, and measurements based on the JIP-test, namely maximum quantum yield of PSII and the performance index. Additionally, we measured leaf functional traits, namely specific leaf area, leaf dry matter content, stomatal pore area index, and chlorophyll content. We assessed all traits weekly on two evergreen species Berberis vulgaris and Quercus × hispanica as well as the two summer green species Betula pendula...
Special issue in honour of Prof. Reto J. Strasser – Characterization of photosynthetic performance during natural leaf senescence in winter wheat: Multivariate analysis as a tool for phenotypic characterization
M. VILJEVAC VULETIĆ, V. ŠPANIĆ
Photosynthetica X:X | DOI: 10.32615/ps.2019.162
In the current research, we used chlorophyll fluorescence measurements and photosynthetic pigment content to evaluate onset and rate of the flag leaf senescence every 7 d beginning at the flowering stage (0 d after flowering, DAF) until late senescence stage (35 DAF) on three winter wheat field-grown varieties (Alka, Žitarka, and Olimpija) with the similar maturation time. OJIP curves implied that flag leaves of variety Alka detained the longest photosynthetic efficiency, while the earliest symptoms of senescence onset were indicated by positive L-band at 7 DAF and K-band at 14 DAF in variety Olimpija. A shift in I-P phase in the wheat flag leaves...
Special issue in honour of Prof. Reto J. Strasser – Seasonal monitoring of PSII functionality and relative chlorophyll content on a field site in two consecutive years: A case study of different oak species
S. KOLLER, V. HOLLAND, W. BRÜGGEMANN
Photosynthetica X:X | DOI: 10.32615/ps.2019.163
In search for practical silvicultural management tools to identify alternative tree species for predicted Central European climate conditions, a cross-species survey with five evergreen, semi-evergreen, and deciduous Quercus taxa with contrasting morphological leaf traits was performed. Fast chlorophyll fluorescence induction of PSII and relative leaf chlorophyll contents were performed to assess the overall plant vitality at any point in time during two complete vegetation periods in consecutive years (2012 and 2013). Maximum photochemical efficiency of PSII and the performance index on absorption base showed a very conservative relationship...
Special issue in honour of Prof. Reto J. Strasser – Efficacy of botanical pesticide for rotifer extermination during the cultivation of Nannochloropsis oculata probed by chlorophyll a fluorescence transient
L.T. ZHANG, R. XU, J.G. LIU
Photosynthetica X:X | DOI: 10.32615/ps.2019.164
Nannochloropsis is widely used in aquaculture as a feed source. However, large-scale cultivation of Nannochloropsis usually fails due to rotifer contamination. In order to identify an effective technique for reducing rotifer contamination, the effect of Brachionus plicatilis contamination on photosynthetic characteristics in Nannochloropsis oculata and the efficacy of the celangulin (CA):toosendanin (TSN) (1:9) combination for rotifer extermination were investigated using chlorophyll a fluorescence transient. B. plicatilis could directly devour microalgal cells and sharply reduced N. oculata density to very...
Special issue in honour of Prof. Reto J. Strasser – Thylakoid membrane reorganization, induced by growth light intensity, affects the plants susceptibility to drought stress
N. PETROVA, M. PAUNOV, S. STOICHEV, S. TODINOVA, S.G. TANEVA, V. GOLTSEV, S. KRUMOVA
Photosynthetica X:X | DOI: 10.32615/ps.2019.165
One of the major questions in photosynthesis research is related to the role of the ultrastructure of thylakoid membranes in higher plants for their functional performance at optimal and stress environmental conditions. In this work, we compared the drought stress response of pea plants grown at moderate and low light conditions, based on our recent findings that they differ in the extent of grana stacking and in the thermal stability of the major light-harvesting complex of PSII that plays important regulatory role for plant survival. Our data demonstrate that low light-adapted plants are structurally and functionally less affected by the water deficit,...
Special issue in honour of Prof. Reto J. Strasser – Analysis of OJIP transients during photoinactivation of photosystem II indicates the presence of multiple photosensitizers in vivo and in vitro
I. IERMAK, M. SZABÓ, A. ZAVAFER
Photosynthetica X:X
Generally, excessive excitation absorbed by the pigments is considered the cause of PSII photodamage. Previous studies of action spectra of PSII photodamage concluded that shorter wavelengths induce more damage, supporting the hypothesis of the existence of more than one photosensitizer. However, the relative influence of different photosensitizers is still inconclusive. In this work, we have revisited this question by inducing PSII photodamage in vivo and in vitro at two different wavelengths (460 and 660 nm) where the net absorption cross section was the same using equal irradiance. To correlate PSII photodamage with each wavelength...
Special issue in honour of Prof. Reto J. Strasser – Effect of AtLFNR1 deficiency on chlorophyll a fluorescence rise kinetics OJIP of Arabidopsis
Y. GUO, Y. ZHANG, Y. LU, J. SHI, S. CHEN, R.J. STRASSER, S. QIANG, Z. HU
Photosynthetica X:X | DOI: 10.32615/ps.2019.167
Leaf-type ferredoxin-NADP(H) oxidoreductase, encoded by AtLFNR1 gene in Arabidopsis, extensively exists in chloroplast stroma and thylakoids and is responsible for the reduction of NADP+ to NADPH in PSI. To investigate the relationship between the characteristics of chlorophyll a fluorescence transients OJIP and the function of AtLFNR1 protein, the fluorescence rise kinetics OJIP curves of AtLFNR1 mutant leaves were determined. It is observed that AtLFNR1 mutant has a lower level of variable fluorescence intensity relative to wild type, especially the J-step and the IP-phase. The JIP-test analysis suggests...
High humidity alleviates photosynthetic inhibition and oxidative damage of tomato seedlings under heat stress
C. XU, Z.Q. YANG, S.Q. YANG, L. WANG, M.T. WANG
Photosynthetica X:X | DOI: 10.32615/ps.2019.168
This study investigated the effects of high humidity on the growth and photosynthetic and physiology traits of tomato plants under high temperature stress (HT). The results showed that high humidity effectively alleviated the limitation of HT on plant growth and increased the root-to-shoot ratio. In addition, high humidity also increased the chlorophyll content, net photosynthetic rate, and maximum photochemical quantum yield of PSII in tomato seedlings under HT stress, but declined the stomatal limitation value. Moreover, JIP-test showed that increasing air humidity improved the quantum yields and efficiencies of HT-stressed tomato plants and increased...
Special issue in honour of Prof. Reto J. Strasser – JIP-test as a tool to identify salinity tolerance in sweet sorghum genotypes
A. RASTOGI, M. KOVAR, X. HE, M. ZIVCAK, S. KATARIA, H.M. KALAJI, M. SKALICKY, U.F. IBRAHIMOVA, S. HUSSAIN, S. MBARKI, M. BRESTIC
Photosynthetica X:X
The effect of salinity on primary photochemical reactions (using JIP-test) in six sweet sorghum genotypes was tested. An increase in salt concentrations induced significantly the accumulation of proline and caused a decline in leaf osmotic potential. Except for 100 mM NaCl concentration, salinity significantly decreased chlorophyll content and photosynthetic efficiency of plants. Increasing salinity led to a higher accumulation of QB-nonreducing PSII reaction centers. K-step in OJIP fluorescence transient was observed for the most sensitive genotypes under the high NaCl concentration. The studied sorghum genotypes responded differently to...
Effects of exogenous cytokinin on photosynthesis, senescence, and yield performance of inferior rice tillers grown under different nitrogen regimes
Y. WANG, J.W. LU, T. REN, P.F. LI, Q.X. LIU, X.K. LI
Photosynthetica X:X | DOI: 10.32615/ps.2019.170
Inferior tillers contribute negligibly to rice grain yields as their late occurrence or prematurity curtails growth. However, 6-benzylaminopurine (6-BA, a synthetic cytokinin) can delay tiller senescence. Our results indicate that foliar application of 6-BA enhanced the distribution and allocation of light energy for the photochemical reactions of PSII, under appropriate N supply. The percentage of thermal energy dissipation also decreased following the application of 6-BA as a consequence of the reduced risk of chloroplast oxidative damage through excess excitations. Moreover, leaf peroxidase, catalase, and ascorbate peroxidase activities were augmented...
Special issue in honour of Prof. Reto J. Strasser – Analysis of drought response of sunflower inbred lines by chlorophyll a fluorescence induction kinetics
Ö. ARSLAN, A.S. BALKAN NALÇAİYİ, Ş. ÇULHA ERDAL, V. PEKCAN, Y. KAYA, N. ÇİÇEK, Y. EKMEKÇİ
Photosynthetica X:X | DOI: 10.32615/ps.2019.171
The aim of the study was to screen nine inbred lines of sunflower by inducing drought for 10 d and subsequent rewatering for 5 d. Impact of drought was determined by chlorophyll fluorescence and some physiological parameters. Drought led to a decrease in the photosynthetic performance, the quantum yield, and efficiency of electron transport in sunflower lines, while it caused an increase in the absorption flux per reaction centre, dissipation of an active reaction centre, and K-band as well as L-band. Drought also decreased the total chlorophyll contents and water status of the lines, which contributed to photoinhibition. Our results suggested that...
Special issue in honour of Prof. Reto J. Strasser – Action of alamethicin in photosystem II probed by the fast chlorophyll fluorescence rise kinetics and the JIP-test
W. XIAO, H. WANG, W. LIU, X. WANG, Y. GUO, R.J. STRASSER, S. QIANG, S. CHEN, Z. HU
Photosynthetica X:X | DOI: 10.32615/ps.2019.172
Alamethicin (AMT) is a linear antimicrobial peptide isolated from fungi Trichoderma viride. To date, the mode of action of AMT in plant cells remains unknown. Our experimental results indicate that AMT causes leaf lesion attributed to its multiple effects on PSII. AMT decreases the O2 evolution rate of PSII. Based on chlorophyll fluorescence data, similar to the classical herbicide diuron, AMT interrupts PSII electron transfer beyond QA at the acceptor side, leading to the inactivation of the PSII reaction centers. Additionally, AMT decreases chlorophyll content and destroys the architecture of PSII pigment assemblies....
Special issue in honour of Prof. Reto J. Strasser – The JIP-test as a tool for forestry in times of climate change
F. BANTIS, E. FRÜCHTENICHT, J. GRAAP, S. STRÖLL, N. REININGER, L. SCHÄFER, M. POLLASTRINI, V. HOLLAND, F. BUSSOTTI, K. RADOGLOU, W. BRÜGGEMANN
Photosynthetica X:X | DOI: 10.32615/ps.2019.173
Replicate common gardens of Italian and Greek provenances of Quercus ilex, Q. pubescens, and Q. frainetto seedlings were established in 2017 in Italy, Greece, and Germany (here: also Q. robur) to assess their potential for plantations for future climate scenarios. The JIP-test was applied on saplings (2016) and in the summers of 2017-2019 to assess the efficiency of the photosynthetic apparatus. Seedlings from the nurseries differed in the tested JIP parameters from older plants in the field. Further, considerable inter-annual and between-sites variability of the parameters occurred. Q. pubescens revealed the most...
Special issue in honour of Prof. Reto J. Strasser – JIP-test as a tool for early diagnostics of plant growth and flowering upon selected light recipe
M. ZHIPONOVA, M. PAUNOV, S. ANEV, N. PETROVA, S. KRUMOVA, A. RAYCHEVA, V. GOLTSEV, N. TZVETKOVA, S. TANEVA, K. SAPUNOV, G. CHANEVA
Photosynthetica X:X | DOI: 10.32615/ps.2019.174
Light is a major factor controlling plant growth and development. To assess the impact of the applied light conditions, we aimed to sort out a tool for early diagnostics of the plant physiological state. We investigated the effect of a blue:red:far-red LED light formula recommended for improved plant performance (flowering). High (BR) and low (BRS) light intensity variants were compared to normal (W) and 'shadowed' (WS) white fluorescent controls. The efficiency of the JIP-test to determine changes during early growth of pea plants was compared to additional physiological characteristics (growth parameters, thermal stability of the thylakoid membranes,...
Changes in photosynthesis and carotenoid composition of pepper (Capsicum annuum L.) in response to low-light stress and low temperature combined with low-light stress
J.F. ZHANG, J. LI, J.M. XIE, J.H YU, M.M. DAWUDA, J. LYV, Z.Q. TANG, J. ZHANG, X.D. ZHANG, C.N. TANG
Photosynthetica X:X | DOI: 10.32615/ps.2019.175
Light and temperature are two major factors for plant growth. Carotenoids are important photoprotective and light-harvesting pigments in photosynthetic organs. Therefore, this study explored photosynthesis and carotenoid-related metabolism under control (CK), low-light (L), and low-temperature and low-light (LL) conditions by using the pepper (Capsicum annuum L.) varieties Longjiao 5 and Hangjiao 4. We found the increased inhibition of plant growth and photosynthetic capacity under both L and LL stress. Meanwhile, carotenoid-related metabolic compounds accumulated in pepper showed different responses to the two stresses. Significantly, zeaxanthin...
Special issue in honour of Prof. Reto J. Strasser – Sporulation modifies the photosynthetic activity of sporotrophophyll leaves of Platycerium bifurcatum
A. SKOCZOWSKI, G. RUT, J. OLIWA, A. KORNAS
Photosynthetica X:X | DOI: 10.32615/ps.2019.176
Platycerium bifurcatum is an epiphytic fern, occurring mainly in the forests of Australia and New Guinea. The location and spatial structure of its sporangia makes this fern a unique research model for physiological experiments. Our research aimed to determine changes in photosynthesis in the sporophilic and trophophilic parts of P. bifurcatum leaves during spore tying and maturation. For this purpose, the JIP-test and gas-exchange measurements of leaves were performed. In the study, we showed that changes typical of plant responses to stress factors also happened at critical periods in plant ontogenesis (e.g., in the generative phase)...
Special issue in honour of Prof. Reto J. Strasser – JIP-test as a tool for early detection of the macronutrients deficiency in Miscanthus plants
T. HORACZEK, P. DĄBROWSKI, H.M. KALAJI, A.H. BACZEWSKA-DĄBROWSKA, S. PIETKIEWICZ, W. STĘPIEŃ, D. GOZDOWSKI
Photosynthetica X:X
The optimization of cultivation process and improving the yield of perspective energy crops in Europe could be one of the ways to overcome the problem of limited amount of conventional fuel. The aim of this work was to check if the use of JIP-test, as a noninvasive method for early detection of the changes in photosynthetic apparatus, can be applied to detect nutrients deficiency in miscanthus (Miscanthus × gigantheus Anderss.) plants. The experiment was performed in fully randomized design with the following experimental variants: CaNPK (full fertilization, control), NPK (without calcium), CaKN (phosphorus poor), CaPK (nitrogen poor),...
Special issue in honour of Prof. Reto J. StrasserChlorophyll fluorescence of Nicotiana tabacum expressing the green fluorescent protein
N. Keutgen, M. Tomaszewska-Sowa, A.J. Keutgen
Photosynthetica X:X | DOI: 10.32615/ps.2019.178
Evidence that the green fluorescence protein (GFP) develops a significant toxicity in plants has not been found, but it may represent a source of free radicals as a consequence of its fluorescence. In addition, green light is known to trigger the acclimatisation of the photosynthetic system towards a shady environment. Moreover, the light-harvesting system may acclimate to an increased availability of green light. Each of these effects may be induced by the GFP. Therefore, the hypothesis was tested, whether transformation of Nicotiana tabacum cv. Bursan to express the GFP could affect chlorophyll fluorescence parameters. The analysis revealed...
Special issue in honour of Prof. Reto J. Strasser – Environmental pollution is reflected in the activity of the photosynthetic apparatus
S. Hajihashemi, M. Brestic, H.M. Kalaji, M. SKALICKY, F. Noedoost
Photosynthetica X:X
The efficiency of chlorophyll (Chl) fluorescence for forecasting plant response to environmental pollution was examined in four wheat cultivars. The plants were irrigated with wastewater from Razi petrochemical complex. A forecasting model was designed based on the data of maximal quantum yield of photosystem II (Fv/Fm), efficiency of both photosystems (PIABS), and Chl contents of 60-d analysis. A comparison of the forecasting model results with the real data of Fv/Fm, PIABS, and Chl in response to wastewater suggested Fv/Fm as an accurate tool for stress forecasting....
Special issue in honour of Prof. Reto J. Strasser – Can we predict winter survival in plants using chlorophyll a fluorescence?
T. SWOCZYNA, J. MOJSKI, A.H. BACZEWSKA-DĄBROWSKA, H.M. KALAJI, N.I. ELSHEERY
Photosynthetica X:X | DOI: 10.32615/ps.2019.181
In the last years, JIP-test became to be a tool widely applied to assess the performance of photosynthetic apparatus of plants growing under environmental stresses. The objective of our work was to check whether JIP-test can help to predict winter survival in plants. An experiment with outdoor vertical garden was conducted in June 2015 on a south-oriented wall in Lublin city, Poland. Plants were cultivated in pockets made of polyester felt and irrigated by automatic controlled system. After winter period (2015/2016) only 16 species and cultivars from 23 initially planted taxa substantiated successful survival. Chlorophyll fluorescence measurements...
Special issue in honour of Prof. Reto J. Strasser – Targets of nitric oxide (NO) during modulation of photosystems in pea mesophyll protoplasts: studies using chlorophyll a fluorescence
B. SUNIL, R.J. STRASSER, A.S. RAGHAVENDRA
Photosynthetica X:X | DOI: 10.32615/ps.2019.183
Plants adapt to the environmental stresses by using the signalling molecules, such as H2O2 and nitric oxide (NO). We have assessed the NO-induced changes by using sodium nitroprusside (SNP, a NO donor) in photosynthetic components in protoplasts of pea (Pisum sativum) by measuring chlorophyll (Chl) a fluorescence induction kinetics. On exposure to SNP during preillumination, their photochemical activities were severely affected. There was a decrease of 40% in O2 evolution by 5 min and complete inhibition by 20 min. The patterns of photosynthesis in absence of NO did not vary much over 20 min. Chl a...
Lanthanum chloride enhances the photosynthetic characteristics and increases konjac glucomannan contents in Amorphophallus sinensis Belval
X.X. LI, B. YU, Y.Y. DONG, L.S. WANG, S.L. ZHANG, H.Y. SHANGGUAN, Z.H. HE, X.M. LUO, P.F. LAI
Photosynthetica X:X | DOI: 10.32615/ps.2019.184
Lanthanum (La) has been used as agricultural inputs in order to enhance yield and improve crop quality. However, little is known about the effect of La on the photosynthesis and growth of Amorphophallus sinensis, a worldwide food source. The effects of La on the photosynthetic and chlorophyll fluorescence parameters, photosynthetic pigments, corm yield, and konjac glucomannan (KGM) of Amorphophallus sinensis were investigated via field experiments. The leaves were sprayed with different concentrations of LaCl3 (20, 80, 160, and 240 mg L-1). The results exhibited an increasing effect of LaCl3 on...
Low temperature and low irradiation induced irreversible damage of strawberry seedlings
C. XU, M.T. WANG, Z.Q. YANG, Q.T. ZHENG
Photosynthetica X:X | DOI: 10.32615/ps.2020.001
Low temperature (LT) and low irradiation (LI) are common factors posing a great risk to plants. The study aimed to elucidate the effects of LT and LI and recovery on the photosynthetic apparatus, photoinhibition of PSII, and reactive oxygen metabolism of strawberry seedlings. The results showed that strawberry growth slowed down or even stopped and total chlorophyll content, stomatal conductance, net photosynthetic rate, and maximal quantum yield of PSII photochemistry decreased, while intercellular CO2 concentration increased under LI, LT, and combined stress (LL). Additionally, JIP-test showed that compared to LI or LT stress, LL-stressed...
Special issue in honour of Prof. Reto J. Strasser – Gradual changes in the photosynthetic apparatus triggered by nitrogen depletion during microalgae cultivation in photobioreactor
T.YU. PLYUSNINA, S.S. KHRUSCHEV, N.S. DEGTEREVA, I.V. KONYUKHOV, A.E. SOLOVCHENKO, M. KOUZMANOVA, V.N. GOLTSEV, G.YU. RIZNICHENKO, A.B. RUBIN
Photosynthetica X:X | DOI: 10.32615/ps.2020.002
Changes of photosynthetic activity during natural depletion of nitrogen in a batch culture of Chlorella vulgaris were studied using chlorophyll a fluorescence-based methods. Complex analysis including JIP-test, multiexponential approximation, and the analysis of differential curves was carried out on the recorded fluorescence transients. Three pivot points were detected in dynamics of the JIP-test parameters during culture growing. We associated these time points with different stages of mineral stress progress. Immediately after nitrogen exhaustion in the cultivation medium, a transient increase in the efficiency of PSII was detected....
Special issue in honour of Prof. Reto J. Strasser – Influence of Cuscuta campestris Yunck. on the photosynthetic activity of Ipomoea tricolor Cav. - in vivo chlorophyll a fluorescence assessment
L. ZAGORCHEV, A. TRAIANOVA, D. TEOFANOVA, J. LI, M. KOUZMANOVA, V. GOLTSEV
Photosynthetica X:X | DOI: 10.32615/ps.2020.004
Cuscuta campestris Yunck. is a parasitic plant, acquiring nutrients from the hosts. Although a generalist, its hosts differ in their susceptibility. Ipomoea tricolor Cav. is a semi-compatible host - C. campestris growth is restricted in infected plants. We aimed to assess the effect of the parasite on this semi-compatible host by using the sensitive JIP-test to follow physiological changes in leaves at different vegetative stages - aged, mature, and newly emerging. The characteristics of the photosynthetic machinery were estimated by 17 parameters, calculated from the prompt chlorophyll a fluorescence. The most sensitive were performance...
Special issue in honour of Prof. Reto J. Strasser – Dorsoventral variation in photosynthesis during leaf senescence probed by chlorophyll a fluorescence induction kinetics in cucumber and maize plants
H.Y. WU, F.Q. DONG, L.A. LIU, L. SHI, W.F. ZHANG, C.D. JIANG
Photosynthetica X:X | DOI: 10.32615/ps.2020.005
Although photosynthesis has been intensively studied during leaf senescence, it is unclear whether the changes of photosynthetic function in various mesophyll tissues can be detected using chlorophyll a fluorescence induction kinetics (CFI) in vivo. To clarify this question, leaf structure and CFI were carefully examined in senescent leaves. The results showed declines in chlorophyll content and photosynthetic rate in both cucumber and maize plants as leaves aged. In cucumber leaves, the number of chloroplasts decreased more rapidly in the palisade tissue compared to the sponge tissue as senescence progressed. Under various pulse light...