Photosynthetica, 2005 (vol. 43), issue 4

Photosynthetica 2005, 43(4):529-534 | DOI: 10.1007/s11099-005-0085-z

Effects of heat treatment on the protein secondary structure and pigment microenvironment in photosystem 1 complex

Z. H. Hu1, Y. N. Xu1, Y. D. Gong2, T. Y. Kuang1
1 Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing, China
2 State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing, China

The protein secondary structure and pigments' microenvironment in photosystem 1 (PS1) complexes were studied in the temperature range of 25-80 °C using Fourier transform infrared (FT-IR) and circular dichroism (CD) spectroscopy, respectively. Quantitative analysis of the component bands of the amide I band (1 700-1 600 cm-1) showed no significant change below 50 °C. However, apparent conformational changes occurred at 60 °C and further continued at 70 and 80 °C accompanied with transitions of secondary structure mainly from α-helix to the β-sheet structures. CD analysis demonstrated that the regular arrangement, viz. protein microenvironment of pigments of PS1 complexes, was destroyed by heat treatment which might come from the changes of protein secondary structure of PS1. The CD signals at 645 nm contributed by chlorophyll (Chl) b of light-harvesting complex 1 (LHC1) were easily destroyed at the beginning of heat treatment (25-60 °C). When temperature reached 70 and 80 °C, the CD signals at 478 nm contributed mainly by Chl b of LHC1 and 498 nm contributed by carotenoids decreased most rapidly, indicating that LHC1 was more sensitive to high temperature than core complexes. In addition, the oxygen uptake rate decreased by 90.81 % at 70 °C and was lost completely at 80 °C showing that heat treatment damaged the regular function of PS1 complexes. This may be attributed to heat-induced changes of pigment microenvironment and protein secondary structure, especially transmembrane α-helix located in PsaA/B of PS1.

Keywords: carotenoids; chlorophyll; circular dichroism; Fourier transform infrared spectra; oxygen uptake; photosystem 2; proteins; spinach; Spinacia

Received: December 6, 2004; Accepted: April 4, 2005; Published: December 1, 2005Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Hu, Z.H., Xu, Y.N., Gong, Y.D., & Kuang, T.Y. (2005). Effects of heat treatment on the protein secondary structure and pigment microenvironment in photosystem 1 complex. Photosynthetica, 43(4), 529-534. doi: 10.1007/s11099-005-0085-z.
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)

    • Open full article

    References

    1. Armond, P.A., Bjorkman, O., Staehelin, L.A.: Dissociation of supramolecular complexes in chloroplast membranes. A manifestation of heat damage to the photosynthetic apparatus. - Biochim. biophys. Acta 601: 433-442, 1980. Go to original source...
    2. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoxidase in Beta vulgaris. - Plant Physiol. 24: 1-15, 1949. Go to original source...
    3. Barber, J., Morris, E., Buchel, C.: Revealing the structure of the photosystem II chlorophyll binding proteins, CP43 and CP47. - Biochim. biophys. Acta 1459: 239-247, 2000. Go to original source...
    4. Bassi, R., Silvestri, M., Dainese, P., Moya, I., Giacometti, G.M.: Effects of a non-ionic detergent on the spectral properties and aggregation state of the light-harvesting chlorophyll a/b protein complex (LHCII). - J. Photochem. Photobiol. B 9: 335-354, 1991. Go to original source...
    5. Bassi, R., Simpson, D.: Chlorophyll-protein complexes of barley photosystem I. - Eur. J. Biochem. 163: 221-230, 1987. Go to original source...
    6. Ben-Shem, A., Frolow, F., Nelson, N.: Crystal structure of plant photosystem I. - Nature 426: 630-635, 2003. Go to original source...
    7. Berry, J., Bjorkman, O.: Photosynthetic response and adaption to temperature in higher plants. - Annu. Rev. Plant Physiol. 31: 491-543, 1980. Go to original source...
    8. Carpentier, R., Larue, B., Leblanc, R.M.: Photoacoustic spectroscopy of Anacystis nidulans. III. Detection of photosynthetic activities. - Arch. Biochem. Biophys. 338: 534-543, 1984. Go to original source...
    9. Chitinis, P.R.: Photosystem I. - Plant Physiol. 111: 661-669, 1996. Go to original source...
    10. Chitinis, P.R.: Photosystem I: function and physiology. - Annu. Rev. Plant Physiol. Plant mol. Biol. 52: 593-626, 2001. Go to original source...
    11. Chitnis, P.R., Xu, Q., Chitnis, V.P., Nechushtai, R.: Function and organization of Photosystem I polypeptides. - Photosynth. Res. 44: 23-40, 1995. Go to original source...
    12. De Las Rivas, J., Barber, J.: Structure and thermal stability of photosystem II reaction centres studied by infrared spectroscopy. - Biochemistry 36: 8897-8903, 1997. Go to original source...
    13. Fromme, P., Jordan, P., Krauß, N.: Structure of photosystem I. - Biochim. biophys. Acta 1507: 5-31, 2001. Go to original source...
    14. Fromme, P., Schubert, W.D., Krauß, N.: Structure of Photosystem I: Suggestions on the docking sites for plastocyanin, ferredoxin and the coordination of P700. - Biochim. biophys. Acta 1187: 99-105, 1994. Go to original source...
    15. Golbeck, J.H.: Structure and function of photosystem I. - Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 293-324, 1992. Go to original source...
    16. Haworth, P., Watson, J.L., Arntzen, C.J.: The detection, isolation and characterization of a light-harvesting complex which is specifically associated with photosystem I. - Biochim. biophys. Acta 724: 151, 1983. Go to original source...
    17. He, W.-Z., Newell, W.R., Haris, P.I., Chapman, D., Barber, J.: Protein secondary structure of the isolated photosystem II reaction centre and conformational changes studied by Fourier transformation infrared spectroscopy. - Biochemistry 30: 4552-4559, 1991. Go to original source...
    18. Hu, Z.H., Xu, Y.N., Jiang, G.Z, Kuang, T.Y.: Degradation and inactivation of photosystem I complexes during linear heating. - Plant Sci. 166: 1177-1183, 2004. Go to original source...
    19. Ilik, P., Kouril, R., Fiala, J., Naus, J., Vacha, F.: Spectral characterization of chlorophyll fluorescence in barley leaves during linear heating analysis of high-temperature fluorescence rise around 60 °C. - J. Photochem. Photobiol. B 59: 103-114, 2000. Go to original source...
    20. Jordan, P., Fromme, P., Klukas, O., Witt, H.T., Saenger, W., Krauß, N.: Three-dimensional structure of cyanobacterial photosystem I at 2.5 Å resolution. - Nature 411: 909-917, 2001. Go to original source...
    21. Klukas, O., Schubert, W.D., Jordan, P., Krauß, N., Fromme, P., Witt, H.T., Saenger, W.: Localization of two phylloquinones, QK and QK', in an improved electron density map of photosystem I at 4-Å resolution. - J. biol. Chem. 274: 7361-7367, 1999a. Go to original source...
    22. Klukas, O., Schubert, W.D., Jordan, P., Krauß, N., Fromme, P., Witt, H.T., Saenger, W.: Photosystem I, an improved model of the stromal subunits PsaC, PsaD, and PsaE. - J. biol. Chem. 274: 7351-7360, 1999b. Go to original source...
    23. Krimm, S., Bandekar, J.: Vibrational spectroscopy and conformation of peptides, polypeptides and proteins. - Adv. Protein Chem. 38: 181-364, 1986. Go to original source...
    24. Lam, E., Ortiz, W., Malkin, R.: Chlorophyll a/b proteins of photosystem I. - FEBS Lett. 168: 10-14, 1984. Go to original source...
    25. Morgan, R., Ivanov, A.G., Williams, J., Khan, M., Huner, N.P.A.: Differential thermal effects on the energy distribution between photosystem II and photosystem I in thylakoid membranes of a psychrophilic and a mesophilic alga. - Biochim. biophys. Acta 1561: 251-265, 2002. Go to original source...
    26. Mullet, J.E., Burke, J.J., Arntzen, C.J.: Chlorophyll proteins of photosystem I. - Plant Physiol. 65: 814-822, 1980. Go to original source...
    27. Nechushtai, R., Eden, A., Cohen, Y., Klein, J.: Introduction to photosystem I: reaction centre function, composition and structure. - In: Ort, D.R., Yocum, C.F. (ed.): Oxygenic Photosynthesis: The Light Reactions. Pp. 289-311. Kluwer Academic Publ., Dordrecht - Boston - London 1996.
    28. Ruan, X., Wei, J., Xu, Q., Wang, J.S., Gong, Y.D., Zhang, X.F., Kuang, T.Y., Zhao, N.M.: Comparison of the effects of Triton X-100 treatment on the protein secondary structure of photosystem I and photosystem II studied by FT-IR spectroscopy. - J. mol. Struct. 525: 97-106, 20000. Go to original source...
    29. Ruban, A.V., Calkoen, F., Kwa, S.L.S., Grondelle, R.V., Horton, P., Dekker, J.P.: Characterization of LHCII in the aggregated state by linear and circular dichroism spectroscopy. - Biochim. biophys. Acta 1321: 61-70, 1997. Go to original source...
    30. Scheller, H.V., Jensen, P.E., Haldrup, A., Lunde, C., Knoetzel, J.: Role of subunits in eukaryotic photosystem I. - Biochim. biophys. Acta 1507: 41-60, 2001. Go to original source...
    31. Schubert, W.D., Klukas, O., Saenger, W., Witt, H.T., Fromme, P., Krauß, N.: A common ancestor for oxygenic and anoxygenic photosynthetic systems: a comparison based on the structural model of photosystem I. - J. mol. Biol. 280: 297-314, 1988.
    32. Shi, H., Xiong, L., Yang, K.Y., Tang, C.Q., Kuang, T.Y., Zhao, N.M.: Protein secondary structure and conformational changes of photosystem II during heat denaturation studied by Fourier transform infrared spectroscopy. - J. mol. Struct. 446: 137-147, 1998. Go to original source...
    33. Somsen, O.J., Grondelle, R.V., Amerongen, H.V.: Spectral broadening of interacting pigments: polarized absorption by photosynthetic proteins. - Biophys. J. 71: 1934-1951, 1996.
    34. Surewicz, W.K., Mantsch, H.H.: New insight into protein secondary structure from resolution-enhanced infrared spectra. - Biochim. biophys. Acta 952: 115-130, 1988. Go to original source...
    35. Takeuchi, T.S., Thornber, J.P.: Heat-induced alterations in thylakoid membrane protein composition in barley. - Aust. J. Plant Physiol. 21: 759-770, 1994. Go to original source...
    36. Wang, M., Zhong, Z.P., Wang, K.B., Li, L.B., Kuang, T.Y.: Isolation of chlorophyll protein complexes from the thylakoid membrane in Nostoc flagelliforme and their spectroscopic characterization. - Acta bot. sin. 42: 684-688, 2000.
    37. Wei, J., Yu, H., Li, L.B., Kuang, T.Y., Wang, J.S., Gong, Y.D., Zhao, N.M.: The photodamage process of pigments and proteins of PS I complexes from Spinacia oleracea L. - Chin. Sci. Bull. 46: 1812-1816, 2001.
    38. Zhang, H., Yamamoto, Y., Ishikawa, Y., Carpentier, R.: Characterization of the secondary structure and thermostability of the extrinsic 16 kilodalton protein of spinach photosystem II by Fourier transform infrared spectroscopy. - J. mol. Struct. 513: 127-132, 1999. Go to original source...
    39. Zhong, Z.P., Wu, Y.H., Xu, J., Feng, L.J., Shi, D.J.: Differentiation of hormogonia and photosynthetic characterization of Nostoc flagelliforme. - Acta bot. sin. 42: 570-575, 2000.

    Return to the content