13.1. 2020,
Laboratory of Biomathematics
Laboratory of Biomathematics
PhD project: Alteration of plasma membrane proteins and lipid composition by oxidative stress; studies of rat brain and model cell lines
Most cell functions occur in or around membranes. Membranes represent a meeting point for lipids and proteins. The reactive intermediates, produced by oxidative stress, can alter the membrane and cause the lipid peroxidation of polyunsaturated fatty acids. Highly reactive products of lipid peroxidation can modify the structure and function of membrane leading to changes in the permeability and fluidity of the lipid bilayer. Oxidation of membrane lipids may also influence the environment and functional efficiency of membrane proteins. Oxidative stress is believed to cause many diseases. Present discussion proposing that oxidative stress mechanisms mediate cognitive, emotional and physiological health.
The aim of the PhD project is to test the validity of two advanced and mutually related hypotheses of Li action in pharmacotherapy of bipolar disorder which are based on modulation of Na+/K+-ATPase activity and oxidative stress (first project) or test the effect of lipoperoxidative damage of plasma membrane on G protein-coupled receptors initiated signalling cascades in connection with opioid addiction (second project).
Candidate’s profile (requirements): MD or MSc or equivalent degree in physiology, cellular biology, biochemistry, molecular biology or pharmacology. Practice at the laboratory of biochemistry, cell biology, pharmacology or molecular biology.
Supervisors: Ing. Miroslava Vošahlíková Ph.D. (Miroslava.Vosahlikova@fgu.cas.cz); Doc. RNDr. Petr Svoboda DrSc. (svobodap@fgu.cas.cz),
Relevant publications:
M. Vosahlikova, H. Ujcikova, M. Hlouskova, S. Musil, L. Roubalova, M. Alda, P. Svoboda (2018) Induction of oxidative stress by long-term treatment of live HEK293 cells with therapeutic concentration of lithium is associated with down-regulation of δ-opioid receptor amount and function. Biochem Pharmacol, 154: 452-463.
M. Vosahlikova, H. Ujcikova, O. Chernyavskiy, J. Brejchova, L. Roubalova, P. Svoboda, Effect of therapeutic concentration of lithium on live HEK293 cells; increase of Na+/K+-ATPase, change of overall protein composition and alteration of surface layer of plasma membrane. Biochim Biophys Acta-Gen Subj. 1861(5 Pt A) (2017) 1099-1112.
M. Vosahlikova, P. Svoboda, Lithium - therapeutic tool endowed with multiple beneficiary effects caused by multiple mechanisms. Acta Neurobiol Exp, 76(1) (2016): 1-19.
Z. Qiusheng, M. Jun, Reactive Oxygen Species and Morphine Addiction. In S.I. Ahmad (editor) Reactive Oxygen Species in Biology and Human Health, CRC Press (2016) 501-512.
U. Banerjee, A. Dasgupta, J. K. Rout, O. P. Singh, Effects of lithium therapy on Na+–K+-ATPase activity and lipid peroxidation in bipolar disorder, Prog Neuro-Psychopharmacol Biol Psychiatry. 37(1) (2012) 56-61.
