Metabolism of bioactive lipids
The Laboratory focuses on the analysis of lipid mediators (eicosanoids, docosanoids, endocannabinoids) using the UPLC-MS/MS method, in particular to identify the source of production of various mediators and their effect on metabolism and immune cells (i.e. adipocytes vs. macrophages). Currently, the lab is exploring new anti-diabetic lipids, fatty acid esters of hydroxy fatty acids and lipid-related metabolic pathways using stable isotopes.
We are always interested to hear from prospective candidates. Please submit a short personal statement detailing your research interests and qualifications, and a CV by email.
OPEN PhD positions:
Targeted analysis of metabolic pathways - fluxomics by LC-MS/MS
New metabolic pathways involved in synthesis of anti-diabetic lipids
Projects
White adipose tissue (WAT) is a complex endocrine organ and its low-grade inflammation in obesity contributes to the development of metabolic disorders. In 2014, a class of WAT-born lipid mediators - fatty acid esters of hydroxy fatty acids (FAHFA) was discovered. FAHFAs are endogenous lipids with anti-inflammatory and anti-diabetic properties, including the enhancement of glucose tolerance, and insulin and glucagon-like peptide 1 (GLP-1) secretion while reducing inflammatory responses [1-5].
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Our lab participates in OPEN SCIENCE initiative and hosts high school students with their project in metabolomics.
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MetaboAnalyst is a great tool for analysis of metabolomics data. Here are some tips how to install MetaboAnalyst locally on a linux workstation. Such installation could be tailored to meet individual requirements (default setting, fonts, etc.).
Starting point is a runnig installation of Ubuntu 18.04.3 LTS.
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CRediT (Contributor Roles Taxonomy) is high-level taxonomy, including 14 roles, that can be used to represent the roles typically played by contributors to scientific scholarly output. The roles describe each contributor’s specific contribution to the scholarly output. This web tool helps to summarize contributions of individual authors and prepare the Author contributions paragraph for a scientific journal.
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The main objective of this project is to explore structure, mechanisms of action, anti-inflammatory and anti-diabetic effects of novel lipokines – branched fatty acid esters of hydroxy fatty acids derived from polyunsaturated fatty acids of n-3 series (Omega-3) using obesity and inflammation models.
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Achievements
Ondrej Kuda has been awarded Lumina Quaeruntur praemium.
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Publications
Vik; A. - Hansen; T. V. - Kuda; Ondřej
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Synthesis of both enantiomers of the docosahexaenoic acid ester of 13-hydroxyoctadecadienoic acid (13-DHAHLA)
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Tetrahedron Letters. 2019; 60(52)); 151331
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IF = 2.259
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Ribot; J. - Arreguín; A. - Kuda; Ondřej - Kopecký; Jan - Palou; A. - Bonet; M. L.
Novel Markers of the Metabolic Impact of Exogenous Retinoic Acid with A Focus on Acylcarnitines and Amino Acids
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International Journal of Molecular Sciences. 2019; 20(15)); 3640
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IF = 4.183
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Pomar, C. A. - Kuda, Ondřej - Kopecký, Jan - Rombaldová, Martina - Castro, H. - Picó, C. - Sánchez, J. - Palou, A.
Alterations in plasma acylcarnitine and amino acid profiles may indicate poor nutrition during the suckling period due to maternal intake of an unbalanced diet and may predict later metabolic dysfunction
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FASEB Journal 2019, 33(1), 796-807
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IF = 5.391
[ASEP]
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Oseeva, Marina - Palůchová, Veronika - Žáček, P. - Janovská, Petra - Mráček, Tomáš - Rossmeisl, Martin - Hamplová, Dana - Čadová, Naděžda - Štohanzlová, Iva - Flachs, Pavel - Kopecký, Jan - Kuda, Ondřej
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Omega-3 index in the Czech Republic: No difference between urban and rural populations
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Chemistry and Physics of Lipids 2019, 220(May), 23-27
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IF = 2.536
[ASEP]
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doi
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Kristyna Magno Ondrej Veronika Marina
Speedvac
Growing cells
QTRAP 5500 / SelexION
Growing cells
Eolid Phase Extraction
